Absolute quantification of prion protein (90-231) using stable isotope-labeled chymotryptic peptide standards in a lc-mrm aqua workflow

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 9, 2012, Pages 1522-1533

  Sturm, Robert ^a   Sheynkman, Gloria ^a   Booth, Clarissa ^a   Smith, Lloyd M ^a   Pedersen, Joel A ^a   Li, Lingjun ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

AQUA; Chymotrypsin; Multiple reaction monitoring (MRM); Prion; Protein quantification; Stable isotope labeled reference for absolute quantitation

Indexed keywords

AQUA; CHYMOTRYPSIN; MULTIPLE REACTION MONITORING; PRION; PROTEIN QUANTIFICATION; STABLE ISOTOPES;

AMINO ACIDS; ANIMAL CELL CULTURE; ASSAYS; CONSERVATION; ISOTOPES; LIQUID CHROMATOGRAPHY; MAMMALS; MASS SPECTROMETRY;

PEPTIDES;

CHYMOTRYPSIN; PRION PROTEIN; PRION PROTEIN [90-231]; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANALYTICAL PARAMETERS; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIOASSAY; CELL CULTURE; CHEMICAL COMPOSITION; HUMAN; IMMUNOLOGICAL TECHNIQUE; IN VITRO STUDY; INFECTION SENSITIVITY; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY MULTIPLE REACTION MONITORING ABSOLUTE QUANTIFICATION; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN CONFORMATION; QUANTITATIVE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHROMATOGRAPHY, LIQUID; CHYMOTRYPSIN; CRICETINAE; HUMANS; ISOTOPE LABELING; LINEAR MODELS; MASS SPECTROMETRY; MICE; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PRIONS; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT;

ANIMALIA; MAMMALIA;

EID: 84865725559     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-012-0411-1     Document Type: Article

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