Green fluorescent protein extraction and LPS removal from escherichia coli fermentation medium using aqueous two-phase micellar system

Separation and Purification Technology

Volumn 81, Issue 3, 2011, Pages 339-346

  Lopes, Andreand́ M ^a   Magalhaandes, Peand́rola O ^b   Mazzola, Priscila G ^c   Rangel Yagui, Carlota O ^a   De Carvalho, Joaando C M ^a   Penna, Thereza C V ^a   Pessoa Jr , Adalberto ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITY OF BRASILIA   (Brazil)

^c UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Green fluorescent protein; Liquid liquid extraction; LPS removal; Protein purification; Triton X 114

Indexed keywords

BIOTECHNOLOGY; ESCHERICHIA COLI; EXTRACTION; FLUORESCENCE; MICELLES; MOLECULAR BIOLOGY; PURIFICATION; RECOMBINANT PROTEINS; FERMENTATION;

AQUEOUS TWO-PHASE MICELLAR SYSTEMS; BACTERIAL EXPRESSION SYSTEMS; EXCLUDED-VOLUME INTERACTIONS; GREEN FLUORESCENT PROTEIN; LIQUID-LIQUID EXTRACTION; PROTEIN PURIFICATION; SEPARATION AND PURIFICATION TECHNIQUES; TRITON X 114;

PROTEINS; MICELLES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84865710488     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2011.07.043     Document Type: Article

References (61)

1. E.M.G. Rodrigues, A. Pessoa-Júnior, A.M.F. Milagres, Screening of variables in xylanase recovery using BDBAC reversed micelles, Appl. Biochem. Biotechnol. 77 (1999) 779-788.
2. E.M.G. Rodrigues, A.M.F. Milagres, A. Pessoa-Júnior, Xylanase recovery: effect of extraction conditions on the AOT reversed micellar systems using experimental design, P. Biochem. 3 (1999) 121-125.
3. L.C. Góes, E.A. Miranda, S.M.A. Bueno, Interaction of histidine-tagged human proinsulin with immobilized nickel ion: effect of chelating ligand and thermodynamics analysis, Colloids Surf. A 369 (2010) 176-185.
4. C.R. Raetz, R.J. Ulevitch, S.D. Wright, C.H. Sibley, A. Ding, C.F. Nathan, Gramnegative endotoxin: an extraordinary lipid with profound effects on eukaryotic signal transduction, FASEB J. 12 (1991) 2652-2660.
5. C. Hirayama, M. Sakata, Chromatographic removal of endotoxin from protein solutions by polymer particles, J. Chromatogr. B 781 (2002) 419-432.
6. Y. Ogikubo, M. Norimatsu, K. Noda, J. Takahashi, M. Inotsume, M. Tsuchiya, Y. Tamura, Evaluation of the bacterial endotoxin test for quantification of endotoxin contamination of porcine vaccines, Biologicals 32 (2004) 88-93.
7. H.H. Yu, I. Nakase, S. Pujals, H. Hirose, G. Tanaka, S. Katayama, M. Imanishi, S. Futaki, Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery, Biochim. Biophys. Acta 1798 (2010) 2249-2257.
8. G. Sakata, K. Yoshimura, I. Sakamoto, M. Todokoro, M. Kunitake, Selective removal of endotoxin from a DNA solution by cross-linked cyclodextrin beads, Anal. Sci. 27 (2011) 213-216.
9. D. Petsch, F.B. Anspach, Endotoxin removal from protein solutions, J. Biotechnol. 76 (2000) 97-119.
10. M. Vaara, M. Nurminen, Outer membrane permeability barrier in Escherichia coli mutants that are defective in the late acyltransferases of lipid A biosynthesis, Antimicrob. Agents Chemother. 43 (1999) 1459-1462.
11. K. Buttenschoen, P. Radermacher, H. Bracht, Endotoxin elimination in sepsis: physiology and therapeutic application, Langenbecks Arch. Surg. 395 (2010) 597-605.
12. F.B. Anspach, Endotoxin removal by affinity sorbents, J. Biochem. Biophys. Methods 49 (2001) 661-681.
13. M.J. Fiske, R.A. Fredenburg, K.R. VanDerMeid, J.C. McMichael, R. Arumugham, Method for reducing endotoxin in Moraxella catarrhalis UspA2 protein preparations, J. Chromatogr. B 753 (2001) 269-278.
14. P. Reichelt, C. Schwarz, M. Donzeau, Single step protocol to purify recombinant proteins with low endotoxin contents, Protein Expr. Purif. 46 (2006) 483-488.
15. S.K. Sharma, Endotoxin detection and elimination in biotechnology, Biotechnol. Appl. Biochem. 1 (1986) 5-22.
16. J.R. Fletcher, P.W. Ramwell, The effects of prostacyclin (PG12) on endotoxin shock and endotoxin-induced platelet aggregation in dogs, Circ. Shock. 7 (1980) 299-308.
17. D.L. Fraker, M.C. Stovroff, M.J. Merino, J.A. Norton, Tolerance to tumor necrosis factor in rats and the relationship to endotoxin tolerance and toxicity, J. Exp. Med. 168 (1988) 95-105.
18. Y.C. Kim, S. Kwon, S.Y. Lee, H.N. Chang, Effect of pLysS on the production of bioadhesive precursor protein by fed-batch cultivation of recombinant Escherichia coli, Biotechnol. Lett. 20 (1998) 799-803.
19. J.H. Lee, High level expression of antimicrobial peptide mediated by a fusion partner reinforcing formation of inclusion bodies, Biochem. Biophys. Res. Comm. 277 (2000) 575-680.
20. S.H. Pyo, J.H. Lee, H.B. Park, S.S. Hong, J.H. Kim, A large-scale purification of recombinant histone H1.5 from Escherichia coli, Protein Expr. Purif. 23 (2001) 38-44.
21. J. Li, G. Shang, M. You, S. Peng, Z. Wang, H. Wu, G.Q. Chen, Endotoxin Removing Method Based on Lipopolysaccharide Binding Protein and Polyhydroxyalkanoate Binding Protein PhaP, Biomacromolecules 12 (2011) 602-608.
22. Y. Kang, R.G. Luo, Chromatographic removal of endotoxin from hemoglobin preparations effects of solution conditions on endotoxin removal efficiency and protein recovery, J. Chromatogr. A 809 (1998) 13-20.
23. P.O. Magalhães, A.M. Lopes, P.G. Mazzola, C.O. Rangel-Yagui, T.C.V. Penna, A. Pessoa-Júnior, Methods of Endotoxin Removal from Biological Preparations: a Review, J. Pharm. Pharm. Sci. 10 (2007) 271-287.
24. N. Arifin, M. Basuni, C.A. Lan, A.R.M. Yahya, R. Noordin, Purification of BmR1 recombinant protein, Protein J. 29 (2010) 509-615.
25. J.H. Choi, K.C. Keum, S.Y. Lee, Production of recombinant proteins by high cell density culture of Escherichia coli, Chem. Eng. Sci. 61 (2006) 876-885.
26. R. Ma, D.D. Fan, W.J. Xue, J.Y. Xing, C.H. Zhu, X.X. Ma, Endotoxin removal during the purification process of human-like collagen, Sep. Sci. Technol. 45 (2010) 2400-2405.
27. S. Liu, R. Tobias, S. McClure, G. Styba, Q. Shi, G. Jackowski, Removal of endotoxin from recombinant protein preparations, Clin. Biochem. 30 (1997) 455-463.
28. F.B. Anspach, O. Hilbeck, Removal of endotoxins by affinity sorbents, J. Chrom. A 711 (1995) 81-92.
29. Z. Wei, W. Huang, J. Li, G. Hou, J. Fang, Z. Yuan, Studies on endotoxin removal mechanism of adsorbents with amino acid ligands, J. Chromatogr. B 852 (2007) 288-292.
30. S.H. Lee, J.S. Kim, C.W. Kim, Optimization of buffer conditions for the removal of endotoxins using Q-sepharose, Process Biochem. 38 (2003) 1091-1098.
31. Y. Aida, M.J. Pabst, Removal of endotoxin from protein solutions by phase separation using Triton X-114, J. Immunol. Methods 14 (1990) 191-195.
32. M. Cotten, A. Baker, M. Saltik, E. Wagner, M. Buschle, Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus, Gene Ther. 1 (1994) 239- 246.
33. O. Adam, A. Vercellone, F. Paul, P.F. Monsan, G. Puzo, A non-degradative route for the removal of endotoxin from exopolysaccharides, Anal. Biochem. 225 (1995) 321-327.
34. L.B. Jensen, A.M. Torp, S.B. Andersen, P.S. Skov, L.K. Poulsen, E.F. Knol, E. van Hoffen, The biological activity of a recombinantly expressed (His)6-tagged peanut allergen (rAra h 1) is unaffected by endotoxin removal, J. Immunol. Methods 335 (2008) 116-120.
35. M.P. Mercaldi, H. Dams-Kozlowska, B. Panilaitis, A.P. Joyce, D.L. Kaplan, Discovery of the Dual Polysaccharide Composition of Emulsan and the Isolation of the Emulsion Stabilizing Component, Biomacromolecules 9 (2008) 1988-1996.
36. A. Rozkov, B. Larsson, S. Gillström, R. Björnestedt, S.R. Schmidt, Large-scale production of endotoxin-free plasmids for transient expression in mammalian cell culture, Biotechnol Bioeng. 99 (2008) 557-666.
37. H.T. Cheng, K.C. Huang, H.Y. Yu, K.J. Gao, X. Zhao, F. Li, J. Town, J.R. Gordon, J.W. Cheng, A new protocol for high-yield purification of recombinant human CXCL8(3-72)K11R/G31P expressed in Escherichia coli, Protein Expression Purif. 61 (2008) 65-72.
38. L. Schädlich, T. Senger, C.J. Kirschning, M. Müller, L. Gissmann, Refining HPV 16 L1 purification from E. coli: reducing endotoxin contaminations and their impact on immunogenicity, Vaccine 27 (2009) 1511-1522.
39. A.M. Lopes, P.O. Magalhães, P.G. Mazzola, C.O. Rangel-Yagui, J.C.M. Carvalho, T.C.V. Penna, A. Pessoa-Júnior, LPS removal from an E. coli fermentation broth using aqueous two-phase micellar system, Biotechnol. Prog. 26 (2010) 1644- 1653.
40. M.F. Lin, C. Williams, M.V. Murray, P.A. Ropp, Removal of lipopolysaccharides from protein-lipopolysaccharide complex by nonflammable solvents, J. Chromatogr. B 816 (2005) 167-174.
41. Y.J. Nikas, C.L. Liu, T. Srivastava, N.L. Abbott, D. Blankschtein, Protein partitioning in two-phase aqueous nonionic micellar solutions, Macromolecules 25 (1992) 4794-4806.
42. C.L. Liu, Y.J. Nikas, D. Blankschtein, Novel bioseparations using two-phase aqueous micellar systems, Biotechnol. Bioeng. 52 (1996) 185-192.
43. A.A. Memon, N. Memon, M.I. Bhangera, Micelle-mediated extraction of chlorogenic acid from Morus laevigata W. leaves, Sep. Purif. Technol. 76 (2010) 179-183.
44. T. Cunha, R. Aires-Barros, Large-scale extraction of proteins, Mol. Biotechnol. 20 (2002) 29-40.
45. P.G. Mazzola, H. Lam, M. Kavoosi, C.A. Haynes, A. Pessoa-Júnior, T.C.V. Penna, D.I.C. Wang, D. Blankschtein, Affinity-tagged green fluorescent protein (GFP) extraction from a clarified E. coli cell lysate using a two-phase aqueous micellar system, Biotechnol. Bioeng. 93 (2006) 998-1004.
46. M. Ishii, J.S. Kunimura, T.C.V. Penna, O. Cholewa, Study on the thermal stability of green fluorescent protein (GFP) in glucose parenteral formulations, Int. J. Pharm. 337 (2007) 109-117.
47. B. Barros-Neto, I.S. Scarminio, R.E. Bruns, Como Fazer Experimentos, third ed., Unicamp, 2007.
48. M.I. Rodrigues, A.F. Iemma, Noções sobre experimentos fatoriais e Estratégia experimental para fatoriais fracionados e delineamento composto central rotacional (DCCR). Planejamento de experimentos e otimização de processos: uma estratégia sequencial de planejamentos, 1rd ed., Casa do Pão Editora, 2005.
49. T.C.V. Penna, E. Chiarini, A. Pessoa Jr., A, Permeation associated with three phase partitioning method on the release of green fluorescent protein (GFPuv) from Escherichia coli, Appl. Biochem. Biotechnol. 105 (2003) 481- 491.
50. G.J. Hoover, A. Mowafi, E. Simko, T.E. Kocal, H.W. Ferguson, M.A. Hayes, Plasma proteins of rainbow trout (Oncorhynchus mykiss) isolated by binding to lipopolysaccharide from Aeromonas salmonicida, Comp. Biochem. Physiol. B Biochem. Mol. Biol. 120 (1998) 559-669.
51. United States Pharmacopeia 24 and National Formulary 19 Bacterial Endotoxins, Test. Suppl. 85 (2000) 2875-2879.
52. P.Å. Albertsson, Partition of Cell Particles and Macromolecules, third ed., Willey-Interscience, New York, 1986.
53. D. Blankschtein, G.M. Thurston, G.B. Benedek, Phenomenological theory of equilibrium thermodynamic properties and phase separation of micellar solutions, J. Chem. Phys. 85 (1986) 7268-7288.
54. G.E.P. Box, R. Holohan, E. Scott, Statistics for Experimenters; an Introduction to Design, Data Analysis, and Model Building, John Wiley and Sons, 1978.
55. G. Savelli, N. Spreti, P. Di Profio, Enzyme activity and stability control by amphiphilic self-organizing systems in aqueous solutions, Curr. Opin. Colloid Interface Sci. 5 (2000) 111-117.
56. P.J. Halling, What can we learn by studying enzymes in non-aqueous media?, Philos Trans. R. Soc. Lond. B Biol Sci. 359 (2004) 1287-1297.
57. L. Lue, D. Blankschtein, A liquid-state theory approach to modeling solute partitioning in phase-separated solutions, Ind. Eng. Chem. Res. 35 (1996) 3032-3043.
58. United States Pharmacopoeia 30, National Formulary 25 (2007).
59. Agência Nacional de Vigilância Sanitária - ANVISA, Guia para qualidade em química analítica: uma assistência a acreditação, Brasília, 2004, p. 80.
60. M. Daneshian, A. Guenther, A. Wendel, H. Thomas, S. Von-Aulock, In vitro pyrogen test for toxic or immunomodulatory drugs, J. Immunol. Methods 131 (2006) 169-175.
61. United States Pharmacopoeia 26, Rockville: United Stated Pharmacopeial Convention, 2002, p. 2569.