The biological activity of a recombinantly expressed (His)6-tagged peanut allergen (rAra h 1) is unaffected by endotoxin removal

Journal of Immunological Methods

Volumn 335, Issue 1-2, 2008, Pages 116-120

  Jensen, Louise Bjerremann ^a,b   Torp, Anna Maria ^a   Andersen, Sven Bode ^a   Skov, Per Stahl ^b   Poulsen, Lars K ^b   Knol, Edward F ^c   van Hoffen, Els ^c  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b Rigshospitalet   (Denmark)

^c UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Cell culture assays; LPS; Recombinant protein

Indexed keywords

ALLERGEN; ENDOTOXIN; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 3; LIPOPOLYSACCHARIDE; T LYMPHOCYTE ANTIGEN; TRITON X 114; TUMOR NECROSIS FACTOR ALPHA;

ANTIGEN SPECIFICITY; ARTICLE; BIOLOGICAL ACTIVITY; CELL PROLIFERATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNE SYSTEM; PEANUT ALLERGY; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN DEPLETION; T LYMPHOCYTE; TOXIN ANALYSIS;

ALLERGENS; BASOPHILS; CELLS, CULTURED; CLONING, MOLECULAR; DOSE-RESPONSE RELATIONSHIP, DRUG; GLYCOPROTEINS; HISTAMINE RELEASE; HISTIDINE; HUMANS; INTERLEUKIN-10; LEUKOCYTES, MONONUCLEAR; LIPOPOLYSACCHARIDES; PLANT PROTEINS; POLYETHYLENE GLYCOLS; PROTEIN BINDING; RECOMBINANT PROTEINS; T-LYMPHOCYTES; TIME FACTORS; TUMOR NECROSIS FACTOR-ALPHA;

ARACHIS HYPOGAEA;

EID: 43049156554     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.02.012     Document Type: Article

References (9)

1. Chen T., Toivanen P., and Vainio O. Suppression of antigen-specific lymphocyte proliferation by Gram-positive bacterial cell walls. APMIS 110 (2002) 490
2. Cirkovic V.T., Thunberg S., Polovic N., Neimert-Andersson T., Gronlund H., van H.M., and Gafvelin G. Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells. Int. Arch. Allergy Immunol. 146 (2007) 1
3. Daftarian P.M., Kumar A., Kryworuchko M., and Az-Mitoma F. IL-10 production is enhanced in human T cells by IL-12 and IL-6 and in monocytes by tumor necrosis factor-alpha. J. Immunol. 157 (1996) 12
4. Liu S., Tobias R., McClure S., Styba G., Shi Q., and Jackowski G. Removal of endotoxin from recombinant protein preparations. Clin. Biochem. 30 (1997) 455
5. Matsumae H., Minobe S., Kindan K., Watanabe T., Sato T., and Tosa T. Specific removal of endotoxin from protein solutions by immobilized histidine. Biotechnol. Appl. Biochem. 12 (1990) 129
6. Mielcarek M., Graf L., Johnson G., and Torok-Storb B. Production of interleukin-10 by granulocyte colony-stimulating factor-mobilized blood products: a mechanism for monocyte-mediated suppression of T-cell proliferation. Blood 92 (1998) 215
7. Rodo J., Goncalves L.A., Demengeot J., Coutinho A., and Penha-Goncalves C. MHC class II molecules control murine B cell responsiveness to lipopolysaccharide stimulation. J. Immunol. 177 (2006) 4620
8. Ruiter B., Tregoat V., M'rabet L., Garssen J., Bruijnzeel-Koomen C.A., Knol E.F., and Hoffen E. Characterization of T cell epitopes in alphas1-casein in cow's milk allergic, atopic and non-atopic children. Clin. Exp. Allergy 36 (2006) 303
9. Simon D., Braathen L.R., and Simon H.U. Increased lipopolysaccharide-induced tumour necrosis factor-alpha, interferon-gamma and interleukin-10 production in atopic dermatitis. Br. J. Dermatol. 157 (2007) 583