Large-scale production of endotoxin-free plasmids for transient expression in mammalian cell culture

Biotechnology and Bioengineering

Volumn 99, Issue 3, 2008, Pages 557-566

  Rozkov, Aleksei ^a   Larsson, Bert ^a   Gillström, Stefan ^a   Björnestedt, Robert ^a   Schmidt, Stefan R ^a  

^a ASTRAZENECA R AND D   (Sweden)

Author keywords

Endotoxin removal; Escherichia coli; Fed batch culture; Fermentation; Plasmid; Plasmid purification

Indexed keywords

BIOMASS; ESCHERICHIA COLI; FERMENTATION; PROTEINS; PURIFICATION;

MAMMALIAN CELL CULTURE; MULTI-FERMENTER CONTROL SYSTEM; PLASMID PURIFICATION;

BATCH CELL CULTURE;

ENDOTOXIN; BACTERIAL DNA;

ALKALINITY; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOMASS; CELL CULTURE; CELL LYSATE; CULTURE MEDIUM; EXTRACTION; FED BATCH CULTURE; GROWTH RATE; NONHUMAN; PLASMID; PROTEIN EXPRESSION; PURIFICATION; ULTRAFILTRATION; VOLUMETRY; ANIMAL; BIOSYNTHESIS; CULTURE TECHNIQUE; ESCHERICHIA COLI; GENETIC TRANSFECTION; HUMAN; ISOLATION AND PURIFICATION; MAMMAL; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY; PILOT STUDY;

ESCHERICHIA COLI; MAMMALIA;

ANIMALS; CELL CULTURE TECHNIQUES; DNA, BACTERIAL; ENDOTOXINS; ESCHERICHIA COLI; HUMANS; INDUSTRIAL MICROBIOLOGY; MAMMALS; PILOT PROJECTS; PLASMIDS; TRANSFECTION;

EID: 38449108659     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21603     Document Type: Article

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