Single step protocol to purify recombinant proteins with low endotoxin contents

Protein Expression and Purification

Volumn 46, Issue 2, 2006, Pages 483-488

  Reichelt, Peter ^a   Schwarz, Christoph ^a   Donzeau, Mariel ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Affinity chromatography; Endotoxin removal; Recombinant proteins

Indexed keywords

DETERGENT; ENDOTOXIN; HYBRID PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY;

ANIMALS; CHROMATOGRAPHY, AFFINITY; DETERGENTS; ENDOTOXINS; ESCHERICHIA COLI; HUMANS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 33645409827     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.09.027     Document Type: Article

References (27)

1. D.B. Smith, and K.S. Johnson Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 1988 31 40
2. C. di Guan, P. Li, P.D. Riggs, and H. Inouye Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein Gene 67 1988 21 30
3. J. Porath, J. Carlsson, I. Olsson, and G. Belfrage Metal chelate affinity chromatography, a new approach to protein fractionation Nature 258 1975 598 599
4. E. Hochuli, H. Dobeli, and A. Schacher New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues J. Chromatogr. 411 1987 177 184
5. J. Schmitt, H. Hess, and H.G. Stunnenberg Affinity purification of histidine-tagged proteins Mol. Biol. Rep. 18 1993 223 230
6. L.Z. Bito Inflammatory effects of endotoxin-like contaminants in commonly used protein preparations Science 196 1977 83 85
7. D.C. Morrison, and R.J. Ulevitch The effects of bacterial endotoxins on host mediation systems. A review Am. J. Pathol. 93 1978 526 617
8. D. Petsch, and F.B. Anspach Endotoxin removal from protein solutions J. Biotechnol. 76 2000 97 119
9. S.H. Pyo, J.H. Lee, H.B. Park, S.S. Hong, and J.H. Kim A large-scale purification of recombinant histone H1.5 from Escherichia coli Protein Expr. Purif. 23 2001 38 44
10. F.B. Anspach, and O. Hilbeck Removal of endotoxins by affinity sorbents J. Chromatogr. A 711 1995 81 92
11. F.B. Anspach Endotoxin removal by affinity sorbents J. Biochem. Biophys. Methods 49 2001 665 681
12. D. Petsch, T.C. Beeskow, F.B. Anspach, and W.D. Deckwer Membrane adsorbers for selective removal of bacterial endotoxin J. Chromatogr. B Biomed. Sci. Appl. 693 1997 79 91
13. S. Liu, R. Tobias, S. McClure, G. Styba, Q. Shi, and G. Jackowski Removal of endotoxin from recombinant protein preparations Clin. Biochem. 30 1997 455 463
14. A.C. Issekutz Removal of gram-negative endotoxin from solutions by affinity chromatography J. Immunol. Methods 61 1983 275 281
15. Y. Aida, and M.J. Pabst Removal of endotoxin from protein solutions by phase separation using Triton X-114 J. Immunol. Methods 132 1990 191 195
16. K.L. Franken, H.S. Hiemstra, K.E. van Meijgaarden, Y. Subronto, J. den Hartigh, T.H. Ottenhoff, and J.W. Drijfhout Purification of his-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent Protein Expr. Purif. 18 2000 95 99
17. A. Gatignol, A. Buckler-White, B. Berkhout, and K.T. Jeang Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR Science 251 1991 1597 1600
18. J. Brady, K.T. Jeang, J. Duvall, and G. Khoury Identification of p40x-responsive regulatory sequences within the human T-cell leukemia virus type I long terminal repeat J. Virol. 61 1987 2175 2181
19. K.T. Jeang, I. Boros, J. Brady, M. Radonovich, and G. Khoury Characterization of cellular factors that interact with the human T-cell leukemia virus type I p40x-responsive 21-base-pair sequence J. Virol. 62 1988 4499 4509
20. K. Omagari, H. Yoshimura, M. Takano, D. Hao, M. Ohmori, A. Sarai, and A. Suyama Systematic single base-pair substitution analysis of DNA binding by the cAMP receptor protein in cyanobacterium Synechocystis sp. PCC 6803 FEBS Lett. 563 2004 55 58
21. H.L. Wang, B.L. Postier, and R.L. Burnap Alterations in global patterns of gene expression in Synechocystis sp. PCC 6803 in response to inorganic carbon limitation and the inactivation of ndhR, a LysR family regulator J. Biol. Chem. 279 2004 5739 5751
22. G. Szyrach, M. Ott, N. Bonnefoy, W. Neupert, and J.M. Herrmann Ribosome binding to the Oxa1 complex facilitates co-translational protein insertion in mitochondria EMBO J. 22 2003 6448 6457
23. F.E. Nargang, M. Preuss, W. Neupert, and J.M. Herrmann The Oxa1 protein forms a homooligomeric complex and is an essential part of the mitochondrial export translocase in Neurospora crassa J. Biol. Chem. 277 2002 12846 12853
24. M. Donzeau, E.L. Winnacker, and M. Meisterernst Specific repression of Tax trans-activation by TAR RNA-binding protein TRBP J. Virol. 71 1997 2628 2635
25. D. Jing, J. Agnew, W.F. Patton, J. Hendrickson, and J.M. Beechem A sensitive two-color electrophoretic mobility shift assay for detecting both nucleic acids and protein in gels Proteomics 3 2003 1172 1180
26. M.J. Wilson, C.L. Haggart, S.P. Gallagher, and D. Walsh Removal of tightly bound endotoxin from biological products J. Biotechnol. 88 2001 67 75
27. D. Petsch, W.D. Deckwer, and F.B. Anspach Proteinase K digestion of proteins improves detection of bacterial endotoxins by the Limulus amebocyte lysate assay: application for endotoxin removal from cationic proteins Anal. Biochem. 259 1998 42 47