Comparison of manual and automated DNA purification for measuring TREC in dried blood spot (DBS) samples with qPCR

Journal of Immunological Methods

Volumn 384, Issue 1-2, 2012, Pages 118-127

  Lang, Pierre Olivier ^a,b   Govind, Sheila ^a   Dramé, Moustapha ^c   Aspinall, Richard ^a  

^a CRANFIELD UNIVERSITY   (United Kingdom)

^b Nescens Centre of Preventive Medicine   (Switzerland)

^c SERVICE DE DERMATOLOGIE   (France)

Author keywords

DNA extraction; Dried blood spots; Quantitative PCR; Real time PCR; Thymic TREC; TREC ratio

Indexed keywords

ALBUMIN; NUCLEIC ACID;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COPY NUMBER VARIATION; DNA EXTRACTION; DNA PURIFICATION; DRIED BLOOD SPOT TESTING; HUMAN; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; OPTICAL DENSITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QIAMP INVESTIGATOR; STORAGE; T CELL RECEPTOR EXCISION CIRCLE;

BIOTECHNOLOGY; CRYOPRESERVATION; DNA; DRIED BLOOD SPOT TESTING; GENE REARRANGEMENT, T-LYMPHOCYTE; HUMANS; LEUKOCYTES, MONONUCLEAR; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; RECEPTORS, ANTIGEN, T-CELL; REPRODUCIBILITY OF RESULTS; SERUM ALBUMIN; TIME FACTORS;

EID: 84865605311     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2012.07.016     Document Type: Article

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