Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores

Optics Express

Volumn 20, Issue 17, 2012, Pages 18525-18536

  Chen, Zhixing ^a   Wei, Lu ^a   Zhu, Xinxin ^a   Min, Wei ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE MICROSCOPY; TISSUE;

ANALYTICAL THEORY; EXCITATION INTENSITY; IMAGING CONTRAST; IMAGING DEPTH; IMAGING-DEPTH LIMIT; INCIDENT LASER; MULTI-PHOTON MICROSCOPY; MULTIPHOTONS; OUT-OF-FOCUS; PHOTOACTIVATABLE; QUANTUM STATE; TISSUE PHANTOM;

FLUOROPHORES;

FLUORESCENT DYE;

ALGORITHM; ARTICLE; COMPUTER ASSISTED DIAGNOSIS; IMAGE ENHANCEMENT; LIGHT; METHODOLOGY; MULTIPHOTON MICROSCOPY; RADIATION EXPOSURE; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

ALGORITHMS; FLUORESCENT DYES; IMAGE ENHANCEMENT; IMAGE INTERPRETATION, COMPUTER-ASSISTED; LIGHT; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84865581641     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.20.018525     Document Type: Article

References (31)

1. S. W. Hell and J. Wichmann, "Breaking the diffraction resolution limit by stimulated emission: stimulatedemission- depletion fluorescence microscopy," Opt. Lett. 19(11), 780-782 (1994).
2. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, "Imaging intracellular fluorescent proteins at nanometer resolution," Science 313(5793), 1642-1645 (2006).
3. S. T. Hess, T. P. Girirajan, and M. D. Mason, "Ultra-high resolution imaging by fluorescence photoactivation localization microscopy," Biophys. J. 91(11), 4258-4272 (2006).
4. M. J. Rust, M. Bates, and X. Zhuang, "Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)," Nat. Methods 3(10), 793-796 (2006).
5. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248(4951), 73-76 (1990).
6. W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21(11), 1369-1377 (2003).
7. F. Helmchen and W. Denk, "Deep tissue two-photon microscopy," Nat. Methods 2(12), 932-940 (2005).
8. R. Yuste, ed., Imaging: A Laboratory Manual (Cold Spring Harbor Laboratory Press, 2010)
9. P. Theer, M. T. Hasan, and W. Denk, "Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifier," Opt. Lett. 28(12), 1022-1024 (2003).
10. P. Theer and W. Denk, "On the fundamental imaging-depth limit in two-photon microscopy," J. Opt. Soc. Am. A 23(12), 3139-3149 (2006).
11. D. Kobat, N. G. Horton, and C. Xu, "In vivo two-photon microscopy to 1.6-mm depth in mouse cortex," J. Biomed. Opt. 16(10), 106014 (2011).
12. N. J. Durr, C. T. Weisspfennig, B. A. Holfeld, and A. Ben-Yakar, "Maximum imaging depth of two-photon autofluorescence microscopy in epithelial tissues," J. Biomed. Opt. 16(2), 026008 (2011).
13. J. Ying, F. Liu, and R. R. Alfano, "Spatial distribution of two-photon-excited fluorescence in scattering media," Appl. Opt. 38(1), 224-229 (1999).
14. N. Ji, D. E. Milkie, and E. Betzig, "Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues," Nat. Methods 7(2), 141-147 (2010).
15. M. Rueckel, J. A. Mack-Bucher, and W. Denk, "Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing," Proc. Natl. Acad. Sci. U.S.A. 103(46), 17137-17142 (2006).
16. D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, "Deep tissue multiphoton microscopy using longer wavelength excitation," Opt. Express 17(16), 13354-13364 (2009).
17. H. Hama, H. Kurokawa, H. Kawano, R. Ando, T. Shimogori, H. Noda, K. Fukami, A. Sakaue-Sawano, and A. Miyawaki, "Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain," Nat. Neurosci. 14(11), 1481-1488 (2011).
18. A. Leray, K. Lillis, and J. Mertz, "Enhanced background rejection in thick tissue with differential-aberration two-photon microscopy," Biophys. J. 94(4), 1449-1458 (2008).
19. M. Fernández-Suárez and A. Y. Ting, "Fluorescent probes for super-resolution imaging in living cells," Nat. Rev. Mol. Cell Biol. 9(12), 929-943 (2008).
20. J. Lippincott-Schwartz and G. H. Patterson, "Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging," Trends Cell Biol. 19(11), 555-565 (2009).
21. M. Heilemann, P. Dedecker, J. Hofkens, and M. Sauer, "Photoswitches: key molecules for subdiffractionresolution fluorescence imaging and molecular quantification," Laser Photon. Rev. 3(1-2), 180-202 (2009).
22. H. L. Lee, S. J. Lord, S. Iwanaga, K. Zhan, H. Xie, J. C. Williams, H. Wang, G. R. Bowman, E. D. Goley, L. Shapiro, R. J. Twieg, J. Rao, and W. E. Moerner, "Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores," J. Am. Chem. Soc. 132(43), 15099-15101 (2010).
23. J. Fölling, V. Belov, R. Kunetsky, R. Medda, A. Schönle, A. Egner, C. Eggeling, M. Bossi, and S. W. Hell, "Photochromic rhodamines provide nanoscopy with optical sectioning," Angew. Chem. Int. Ed. Engl. 46(33), 6266-6270 (2007).
24. D. Kleinfeld, P. P. Mitra, F. Helmchen, and W. Denk, "Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex," Proc. Natl. Acad. Sci. U.S.A. 95(26), 15741-15746 (1998).
25. T. J. Mitchison, K. E. Sawin, J. A. Theriot, K. Gee, and A. Mallavarapu, "Caged fluorescent probes," Methods Enzymol. 291, 63-78 (1998).
26. G. H. Patterson and J. Lippincott-Schwartz, "A photoactivatable GFP for selective photolabeling of proteins and cells," Science 297(5588), 1873-1877 (2002).
27. M. Schneider, S. Barozzi, I. Testa, M. Faretta, and A. Diaspro, "Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region," Biophys. J. 89(2), 1346-1352 (2005).
28. Y. Zhao, Q. Zheng, K. Dakin, K. Xu, M. L. Martinez, and W.-H. Li, "New caged coumarin fluorophores with extraordinary uncaging cross sections suitable for biological imaging applications," J. Am. Chem. Soc. 126(14), 4653-4663 (2004).
29. R. Ando, H. Mizuno, and A. Miyawaki, "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting," Science 306(5700), 1370-1373 (2004).
30. M.-Q. Zhu, G.-F. Zhang, C. Li, M. P. Aldred, E. Chang, R. A. Drezek, and A. D. Q. Li, "Reversible two-photon photoswitching and two-photon imaging of immunofunctionalized nanoparticles Targeted to Cancer Cells," J. Am. Chem. Soc. 133(2), 365-372 (2011).
31. L. Wei, Z. Chen, and W. Min, "Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging," Biomed. Opt. Express 3(6), 1465-1475 (2012).