In vitro exposures in diesel exhaust atmospheres: Resuspension of PM from filters versus direct deposition of PM from air

Environmental Science and Technology

Volumn 46, Issue 16, 2012, Pages 9062-9070

  Lichtveld, Kim M ^a   Ebersviller, Seth M ^a   Sexton, Kenneth G ^a   Vizuete, William ^a   Jaspers, Ilona ^b   Jeffries, Harvey E ^a  

^a University of North Carolina   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEX MIXTURE; CONTROLLED TESTS; DIESEL EXHAUSTS; DIRECT DEPOSITION; EXPOSURE CONCENTRATION; FILTER SAMPLE; HEALTH EFFECTS; IN-VITRO; INFLAMMATORY RESPONSE; LIQUID MEDIUM; PARTICULATE MATTER; POST TREATMENT; RE-SUSPENSION; TEST ATMOSPHERES;

CELL CULTURE; LIQUIDS;

TOXICITY;

CARBONYL DERIVATIVE; INTERLEUKIN 8;

ATMOSPHERIC POLLUTION; CHEMICAL ANALYSIS; CONCENTRATION (COMPOSITION); DIESEL ENGINE; EXHAUST EMISSION; FILTER; HEALTH IMPACT; PARTICULATE MATTER; POLLUTION EXPOSURE; RESUSPENSION; SEPARATION; TOXICITY;

AIR; AIR ANALYSIS; AIR POLLUTION; ARTICLE; ATMOSPHERE; CHEMICAL ANALYSIS; CONCENTRATION (PARAMETERS); ENVIRONMENTAL EXPOSURE; EXHAUST GAS; FILTER; HUMAN; HUMAN CELL; IN VITRO STUDY; ISOLATION PROCEDURE; PARTICULATE MATTER; PROTEIN EXPRESSION;

AIR POLLUTANTS; ATMOSPHERE; CELL LINE, TUMOR; CYCLOOXYGENASE 2; HUMANS; INTERLEUKIN-8; RNA, MESSENGER; VEHICLE EMISSIONS;

EID: 84865491756     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es301431s     Document Type: Article

References (54)

1. Abe, S.; Takizawa, H.; Sugawara, I.; Kudoh, S. Diesel Exhaust (DE)-Induced Cytokine Expression in Human Bronchial Epithelial Cells: A Study with a New Cell Exposure System to Freshly Generated DE in Vitro Am. J. Respir. Cell Mol. Biol. 2000, 22, 296-303 (Pubitemid 30343356)
2. Bauling, A.; Sourdeval, M.; Meyer, M.; Marano, F.; Baeza-Squiban, A. Biological Effects of Atmospheric Particles on Human Bronchial Epithelial Cells. Comparison with Diesel Exhaust Particles Toxicol. in Vitro 2003, 17, 567-573 (Pubitemid 37352664)
3. Bayram, H.; Devalia, J. L.; Sapsford, R. J.; Ohtoshi, T.; Miyabara, Y.; Sagai, M.; Davies, R. J. The Effect of Diesel Exhaust Particles on Cell Function and Release of Inflammatory Mediators from Human Bronchial Epithelial Cells In Vitro Am. J. Respir. Cell Mol. Biol. 1998, 18 (3) 441-448 (Pubitemid 128354103)
4. Boland, S.; Baeza-Squiban, A.; Fournier, T.; Houcine, O.; Gendron, M. C.; Chévrier, M.; Jouvenot, G.; Coste, A.; Aubier, M.; Marano, F. Diesel Exhaust Particles Are Taken up by Human Airway Epithelial Cells in Vitro and Alter Cytokine Production Am. J. Physiol.: Lung Cell. Mol. Physiol. 1999, 276, L604-L613
5. Dybdahla, M.; Risom, L.; Bornholdt, J.; Autrup, H.; Loft, S.; Wallin, H. Inflammatory and Genotoxic Effects of Diesel Particles in Vitro and in Vivo Mutat. Res. 2004, 562, 119-131 (Pubitemid 38981786)
6. Knebel, J. W.; Ritter, D.; Aufderheide, M. Exposure of Human Lung Cells to Native Diesel Motor Exhaust-Development of an Optimized in Vitro Test Strategy Toxicol. in Vitro 2002, 16, 185-192 (Pubitemid 34178427)
7. Mazzarella, G.; Ferraraccio, F.; Prati, M. V.; Annunziata, S.; Bianco, A.; Mezzogiorno, A.; Liguori, G.; Angelillo, I. F.; Cazzola, M. Effects of Diesel Exhaust Particles on Human Lung Epithelial Cells: An in Vitro Study Respir. Med. 2007, 101, 1155-1162 (Pubitemid 46667679)
8. Donahue, N.; Robinson, A.; Stanier, C.; Pandis, S. Coupled Partitioning, Dilution, and Chemical Aging of Semivolatile Organics Environ. Sci. Technol. 2006, 40 (8) 2635-2643
9. Pankow, J. Further Discussion of the Octanol/Air Partition Coefficient Koa Correlating Parameter for Gas/Particle Partitioning Coefficients Atmos. Environ. 1998, 32 (9) 1493-1497 (Pubitemid 28241457)
10. Pankow, J.; Liang, C.; Odum, J.; Seinfeld, J. Gas/Particle Partitioning of Semivolatile Organic Compounds to Model Inorganic, Organic, and Ambient Smog Aerosols Environ. Sci. Technol. 1997, 31 (11) 3086-3092 (Pubitemid 27500265)
11. Schwarzenbach, R.; Gschwend, P.; Imboden, D. Environmental Organic Chemistry, 2 nd ed.; Wiley-Interscience: Hoboken, NJ, 2003.
12. Kamens, R.; Jang, M.; Chien, C.-J.; Leach, K. Aerosol Formation from the Reaction of r-Pinene and Ozone Using a Gas-Phase Kinetics-Aerosol Partitioning Model Environ. Sci. Technol. 1999, 33, 1430-1438 (Pubitemid 29217828)
13. Zhou, J.; Ito, K.; Lall, R.; Lippmann, M.; Thurston, G. Time-Series Analysis of Mortality Effects of Fine Particulate Matter Components in Detroit and Seattle Environ. Health Perspect. 2011, 119 (4) 461-466
14. Godleski, J. J.; Rohr, A. C.; Coull, B. A.; Kang, C. M.; Diaz, E. A.; Koutrakis, P. Toxicological Evaluation of Realistic Emission Source Aerosols (TERESA): Summary and Conclusions Inhalation Toxicol. 2011, 23 (Suppl 2) 95-103
15. Chen, X.; Hopke, P. K.; Carter, W. P. Secondary Organic Aerosol from Ozonolysis of Biogenic Volatile Organic Compounds: Chamber Studies of Particle and Reactive Oxygen Species Formation Environ. Sci. Technol. 2011, 45 (1) 276-282
16. McDonald, J. D.; Doyle-Eisele, M.; Campen, M. J.; Seagrave, J.; Holmes, T.; Lund, A.; Surratt, J. D.; Seinfeld, J. H.; Rohr, A. C.; Knipping, E. M. Cardiopulmonary Response to Inhalation of Biogenic Secondary Organic aerosol Inhalation Toxicol. 2010, 22 (3) 253-265
17. Gaschen, A.; Lang, D.; Kalberer, M.; Savi, M.; Geiser, T.; Gazdhar, A.; Lehr, C. M.; Bur, M.; Dommen, J.; Baltensperger, U.; Geiser, M. Cellular Responses after Exposure of Lung Cell Cultures to Secondary Organic Aerosol Particles Environ. Sci. Technol. 2010, 44 (4) 1424-1430
18. Franklin, M.; Schwartz, J. The Impact of Secondary Particles on the Association between Ambient Ozone and Mortality Environ. Health Perspect. 2008, 116 (4) 453-458 (Pubitemid 351864302)
19. Baltensperger, U.; Dommen, J.; Alfarra, M. R.; Duplissy, J.; Gaeggeler, K.; Metzger, A.; Facchini, M. C.; Decesari, S.; Finessi, E.; Reinnig, C.; Schott, M.; Warnke, J.; Hoffmann, T.; Klatzer, B.; Puxbaum, H.; Geiser, M.; Savi, M.; Lang, D.; Kalberer, M.; Geiser, T. Combined Determination of the Chemical Composition and of Health Effects of Secondary Organic Aerosols: The POLYSOA Project J. Aerosol Med. Pulm. Drug Delivery 2008, 21 (1) 145-154
20. Jang, M.; Ghio, A. J.; Cao, G. Exposure of BEAS-2B Cells to Secondary Organic Aerosol Coated on Magnetic Nanoparticles Chem. Res. Toxicol. 2006, 19 (8) 1044-1050 (Pubitemid 44384834)
21. Mar, T. F.; Norris, G. A.; Koenig, J. Q.; Larson, T. V. Associations between Air Pollution and Mortality in Phoenix, 1995-1997 Environ. Health Perspect. 2000, 108 (4) 347-353 (Pubitemid 30251839)
22. Steenhof, M.; Gosens, I.; Strak, M.; Godri, K. J.; Hoek, G.; Cassee, F. R.; Mudway, I. S.; Kelly, F. J.; Harrison, R. M.; Lebret, E.; Brunekreef, B.; Janssen, N. A.; Pieters, R. H. In Vitro Toxicity of Particulate Matter (PM) Collected at Different Sites in the Netherlands Is Associated with PM Composition, Size Fraction and Oxidative Potential - the RAPTES Project Part. Fibre Toxicol. 2011, 8, 26
23. Steerenberg, P. A.; van Amelsvoort, L.; Lovik, M.; Hetland, R. B.; Alberg, T.; Halatek, T.; Bloemen, H. J.; Rydzynski, K.; Swaen, G.; Schwarze, P.; Dybing, E.; Cassee, F. R. Relation between Sources of Particulate Air Pollution and Biological Effect Parameters in Samples from Four European Cities: An Exploratory Study Inhalation Toxicol. 2006, 18 (5) 333-346 (Pubitemid 43336296)
24. Kamens, R.; Coe, D. L.; Large Gas-Phase, A Stripping Device To Investigate Rates of PAH Evaporation from Airborne Diesel Soot Particles Environ. Sci. Technol. 1997, 31 (6) 1830-1833 (Pubitemid 27242001)
25. de Bruijne, K.; Ebersviller, S.; Sexton, K. G.; Lake, S.; Leith, D.; Goodman, R.; Jetters, J.; Walters, G.; Doyle, M.; Woodside, R.; Jeffries, H. E.; Jaspers, I. Design and Testing of Electrostatic Aerosol in vitro Exposure System (EAVES): An Alternative Exposure System for Particles Inhalation Toxicol. 2009, 21 (2) 91-101
26. Doyle, M.; Sexton, K. G.; Jeffries, H. E.; Bridge, K.; Jaspers, I. Effects of 1-3-Butadiene, Isoprene, and Their Photochemical Degradation Products on Human Lung Cells Environ. Health Perspect. 2004, 112, 1488-1495 (Pubitemid 39517442)
27. Doyle, M.; Sexton, K. G.; Jeffries, H. E.; Jaspers, I. Atmospheric Photochemical Transformations Enhance 1,3-Butadiene-Induced Inflammatory Responses in Human Epithelial Cells: The Role of Ozone and Other Photochemical Degradation Products Chem. Biol. Interact. 2007, 166 (1-3) 163-169 (Pubitemid 46413212)
28. Sexton, K. G.; Jeffries, H. E.; Jang, M.; Kamens, R. M.; Doyle, M.; Voicu, I.; Jaspers, I. Photochemical Products in Urban Mixtures Enhance Inflammatory Responses in Lung Cells Inhalation Toxicol. 2004, 16 Suppl 1, 107-114 (Pubitemid 38923907)
29. de Bruijne, K.; Ebersviller, S.; Sexton, K. G.; Olenick, C.; Woodside, R.; Casper, S.; Jaspers, I.; Jeffries, H. E. Comparing the Toxicity of Fresh and Aged Diesel Exhaust using Sparate Particle and Gaseous Exposure Systems The Toxicologist: Supplement to Toxicological Sciences 2008, 102 (1) 224
30. de Bruijne, K.; Sexton, K. G.; Ebersviller, S.; Doyle, M.; Jeffries, H. E. Comparing the Toxicity of Fresh and Aged Diesel Exhaust using a Newly Developed in Vitro Exposure System The Toxicologist: Supplement to Toxicological Sciences 2007, 96 (1) 107
31. de Bruijne, K.; Sexton, K. G.; Ebersviller, S.; Lin, Y.-H.; Jaspers, I.; Jeffries, H. E. Comparing the Toxicity of Fresh and Aged Biodiesel and Diesel Exhaust using Separate Particle and Gaseous Exposure Systems The Toxicologist: Supplement to Toxicological Sciences 2009, 108, 42
32. Ebersviller, S.; Sexton, K. G.; de Bruijne, K.; Woodside, R.; Olenick, C.; Jaspers, I.; Jeffries, H. E. Gas-Particle Partitioning in Urban Air Mixtures and its Effect on Human Epithelial Lung Cells The Toxicologist: Supplement to Toxicological Sciences 2008, 102 (1) 60
33. Ebersviller, S.; Lichtveld, K.; Sexton, K. G.; Zavala, J.; Lin, Y.-H.; Jaspers, I.; Jeffries, H. E. Gaseous VOCs Rapidly Modify Particulate Matter and its Biological Effects: Part 2, Complex Urban VOCs and Model PM Atmos. Chem. Phys. Discussions 2012b, 12, 7365-7418
34. Ebersviller, S.; Lichtveld, K.; Sexton, K. G.; Zavala, J.; Lin, Y.-H.; Jaspers, I.; Jeffries, H. E. Gaseous VOCs Rapidly Modify Particulate Matter and Its Biological Effects-Part 1: Simple VOCs and Model PM Atmos. Chem. Phys. Discussions 2012a, 12, 1-40
35. Seagrave, J.; Dunaway, S.; McDonald, J. D.; Mauderly, J. L.; Hayden, P.; Stidley, C. Responses of Differentiated Primary Human Lung Epithelial Cells to Exposure to Diesel Exhaust at an Air-Liquid Interface Exp. Lung Res. 2007, 33 (1) 27-51 (Pubitemid 46423238)
36. Morin, J.-P.; Fouquet, F.; Monteil, C.; Le Prieur, E.; Vaz, E.; Dionnet, F. Development of a New in Vitro System for Continuous in Vitro Exposure of Lung Tissue to Complex Atmospheres: Application to diesel Exhaust Toxicology Cell Biol. Toxicol. 1999, 15, 143-152 (Pubitemid 29518615)
37. Aufderheide, M.; Mohr, U. CULTEX - An Alternative Technique for Cultivation and Exposure of Cells of the Respiratory Tract to Airborne Pollutants at the Air/Liquid Interface Exp. Toxicol. Pathol. 2000, 52 (3) 265-270 (Pubitemid 30457294)
38. Savi, M.; Kalberer, M.; Lang, D.; Ryser, M.; Fierz, M.; Gaschen, A.; Ricka, J.; Geiser, M. A Novel Exposure System for the Efficient and Controlled Deposition of Aerosol Particles onto Cell Cultures Environ. Sci. Technol. 2008, 42, 5667-5674
39. Sillanpää, M.; Geller, M. D.; Phuleria, H. C.; Sioutas, C. High Collection Efficiency Electrostatic Precipitator for in Vitro Cell Exposure to Concentrated Ambient Particulate Matter (PM) J. Aerosol Sci. 2008, 39, 335-347
40. Volckens, J.; Dailey, L.; Walters, G.; Devlin, R. Direct Particle-to-Cell Deposition of Coarse Ambient Particulate Matter Increases the Production of Inflammatory Mediators from Cultured Human Airway Epithelial Cells Environ. Sci. Technol. 2009, 43, 4595-4599
41. Gaschen, A.; Lang, D.; Kalberer, M.; Savi, M.; Geiser, T.; Gazdhar, A.; Lehr, C.-M.; Bur, M.; Dommen, J.; Baltensperger, U.; Geiser, M. Cellular Reponse after Exposure of Lung Cell Cultures to Secondary Organic Aerosol Particles Environ. Sci. Technol. 2010, 44, 1424-1430
42. McDonald, J.; Barr, E.; White, R.; Chow, J.; Schauer, J.; Zielinska, B.; Grosjean, E. Generation and Characterization of Four Dilutions of Diesel Engine Exhaust for a Subchronic Inhalation Study Environ. Sci. Technol. 2004, 38 (9) 2513-2522 (Pubitemid 38579851)
43. Seaman, V. Y.; Charles, M. J.; Cahill, T. M. A Sensitive Method for the Quantification of Acrolein and Other Volatile Carbonyls in Ambient Air Anal. Chem. 2006, 78 (7) 2405-2412
44. Yu, J.; Jeffries, H.; Sexton, K. G. Atmospheric Photo Oxidation of Alkylbenzenes-I. Carbonyl Product Analyses Atmos. Environ. 1997, 31 (15) 2261-2280
45. Yu, J.; Jeffries, H. E.; Lacheur, R. L. Identifying Airborne Carbonyl Compounds in Isoprene Atmospheric Photo oxidation Products by their PFBHA Oximes Using Gas Chromatography/Ion Trap Mass Spectrometry Environ. Sci. Technol. 1995, 29, 1923-1932
46. Liu, H.; Jeffries, H. E.; Sexton, K. G. Atmospheric Photochmical Degradation of 1,4-Unsaturated Dicarbonyls Environ. Sci. Technol. 1999, 33, 4212-4220
47. ATCC Cell Biology, Designation: A549. Website: http://www.atcc.org (accessed December 10, 2011).
48. Lieber, M.; Smith, B.; Szakal, A.; Nelson-Rees, W.; Todaro, G. A Continuous Tumor-Cell Line from a Human Lung Carcinoma with Properties of Type II Alveolar Epithelial Cells Int. J. Cancer 1976, 17 (1) 62-70
49. Rager, J. E.; Lichtveld, K.; Ebersviller, S.; Smeester, L.; Jaspers, I.; Sexton, K. G.; Fry, R. C.; Toxicogenomic, A. Comparison of Primary and Photochemically Altered Air Pollutant Mixtures Environ. Health Perspect. 2011, 119 (11) 1583-1589
50. Jaspers, I.; Zhang, W.; Fraser, A.; Samet, J. M.; Reed, W. Hydrogen Peroxide Has Opposing Effects on IKK activity and IKappaBalpha Breakdown in Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol. 2001, 24 (6) 769-777 (Pubitemid 32554616)
51. Finlayson-Pitts, B. J.; Pitts, J. N., Jr. Chemistry of The Upper and Lower Atmosphere; Academic Press: New York, 1999; p 969.
52. Atkinson, R. Atmospheric Chemistry of VOCs and NOx Atmos. Environ. 2000, 34, 2063-2101
53. Cooney, D. J.; Hickey, A. J. Cellular Response to the Deposition of Diesel Exhaust Particle Aerosols onto Human Lung Cells Grown at the Air-Liquid Interface by Intertial Impaction Toxicol. in Vitro 2011, 25, 1953-1965
54. Robinson, A.; Donahue, N.; Shrivastava, M.; Weitkamp, E.; Sage, A.; Grieshop, A.; Lane, T.; Pierce, J.; Pandis, S. Rethinking Organic Aerosols: Semivolatile Emissions and Photochemical Aging Science 2007, 315, 1259-1262 (Pubitemid 46364260)