Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37 °C in presence of several antioxidants

Theriogenology

Volumn 78, Issue 5, 2012, Pages 1005-1019

  Mata Campuzano, M ^a   Alvarez Rodriguez M ^a   del Olmo, E ^b   Fernandez Santos M R ^b   Garde, J J ^b   Martinez Pastor F ^a  

^a UNIVERSITY OF LEÓN   (Spain)

^b INSTITUTO DE INVESTIGACIÓN EN RECURSOS CINEGÉTICOS IREC CSIC UCLM JCCM   (Spain)

Author keywords

Antioxidant; DNA damage; Oxidative stress; Red deer; Spermatozoa

Indexed keywords

ANTIOXIDANT; BIOLOGICAL MARKER; MALONALDEHYDE; REACTIVE OXYGEN METABOLITE;

ANIMAL; ARTICLE; DEER; DNA FRAGMENTATION; FREEZING; LIPID PEROXIDATION; MALE; METHODOLOGY; OXIDATIVE STRESS; PHYSIOLOGY; SPERM PRESERVATION; SPERMATOZOON; TEMPERATURE;

ANIMALS; ANTIOXIDANTS; BIOLOGICAL MARKERS; DEER; DNA FRAGMENTATION; FREEZING; LIPID PEROXIDATION; MALE; MALONDIALDEHYDE; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; SEMEN PRESERVATION; SPERMATOZOA; TEMPERATURE;

CERVUS ELAPHUS;

EID: 84865410899     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2011.12.018     Document Type: Article

References (61)

1. Hughes C.M., Lewis S.E., McKelvey-Martin V.J., Thompson W. The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. Hum Reprod 1998, 13:1240-1247.
2. Domínguez-Rebolledo A.E., Fernández-Santos M.R., García-Alvarez O., Maroto-Morales A., Garde J.J., Martínez-Pastor F. Washing increases the susceptibility to exogenous oxidative stress in red deer spermatozoa. Theriogenology 2009, 72:1073-1084.
3. Roca J., Rodríguez M.J., Gil M.A., Carvajal G., Garcia E.M., Cuello C., et al. Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. J Androl 2005, 26:15-24.
4. Gadea J., García-Vazquez F., Matás C., Gardón J.C., Cánovas S., Gumbao D. Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function. J Androl 2005, 26:396-404.
5. Fernández-Santos M.R., Martínez-Pastor F., García-Macías V., Esteso M.C., Soler A.J., Paz P., et al. Sperm characteristics and DNA integrity of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa frozen in the presence of enzymatic and nonenzymatic antioxidants. J Androl 2007, 28:294-305.
6. Berlinguer F., Ledda S., Rosati I., Bogliolo L., Leoni G., Naitana S. Superoxide dismutase affects the viability of thawed European mouflon (Ovis g. Musimon) semen and the heterologous fertilization using both ivf and intracytoplasmatic sperm injection. Reprod Fertil Dev 2003, 15:19-25.
7. Gadea J., Gumbao D., Matás C., Romar R. Supplementation of the thawing media with reduced glutathione improves function and the in vitro fertilizing ability of boar spermatozoa after cryopreservation. J Androl 2005, 26:749-756.
8. Fernández-Santos M.R., Domínguez-Rebolledo A.E., Esteso M.C., Garde J.J., Martínez-Pastor F. Catalase supplementation on thawed bull spermatozoa abolishes the detrimental effect of oxidative stress on motility and DNA integrity. Int J Androl 2009, 32:353-359.
9. Domínguez-Rebolledo A.E., Fernández-Santos M.R., Bisbal A., Ros-Santaella J.L., Ramón M., Carmona M., et al. Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reprod Fertil Dev 2010, 22:856-870.
10. Vera J.C., Rivas C.I., Fischbarg J., Golde D.W. Mammalian facilitative hexose transporters mediate the transport of dehydroascorbic acid. Nature 1993, 364:79-82.
11. Cárcamo J.M., Golde D.W. Vitamin C enters mitochondria via facilitative glucose transporter 1 (Glut1) and confers mitochondrial protection against oxidative injury. FASEB J 2005, 19:1657-1667.
12. Angulo C., Rauch M.C., Droppelmann A., Reyes A.M., Slebe J.C., Delgado-López F., et al. Hexose transporter expression and function in mammalian spermatozoa: cellular localization and transport of hexoses and vitamin C. J Cell Biochem 1998, 71:189-203.
13. Donnelly E.T., McClure N., Lewis S.E. The effect of ascorbate and alpha-tocopherol supplementation in vitro on dna integrity and hydrogen peroxide-induced dna damage in human spermatozoa. Mutagenesis 1999, 14:505-512.
14. Ball B.A., Medina V., Gravance C.G., Baumbe J. Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5 degrees C. Theriogenology 2001, 56:577-589.
15. Fernández-Santos M.R., Domínguez-Rebolledo A.E., Esteso M.C., Garde J.J., Martínez-Pastor F. Refrigerated storage of red deer epididymal spermatozoa in the epididymis, diluted and with vitamin C supplementation. Reprod Domest Anim 2009, 44:212-220.
16. Monteiro J.C., Gonçalves J.S.A., Rodrigues J.A., Lúcio C.F., Silva L.C.G., Assumpção M.E.O.A., et al. Influence of ascorbic acid and glutathione antioxidants on frozen-thawed canine semen. Reprod Domest Anim 2009, 44(Suppl 2):359-362.
17. Cabrita E., Diogo P., Martínez-Páramo S., Sarasquete C., Dinis M.T., Dinis M.T. The influence of certain aminoacids and vitamins on post-thaw fish sperm motility, viability and DNA fragmentation. Anim Reprod Sci 2011, 125:189-195.
18. Mitchell J.B., Samuni A., Krishna M.C., DeGraff W.G., Ahn M.S., Samuni U., et al. Biologically active metal-independent superoxide dismutase mimics. Biochemistry 1990, 29:2802-2807.
19. Mara L., Accardo C., Pilichi S., Dattena M., Chessa F., Chessa B., et al. Benefits of TEMPOL on ram semen motility and in vitro fertility: a preliminary study. Theriogenology 2005, 63:2243-2253.
20. Mara L., Dattena M., Pilichi S., Sanna D., Branca A., Cappai P. Effect of different diluents on goat semen fertility. Anim Reprod Sci 2007, 102:152-157.
21. Foote R.H., Brockett C.C., Kaproth M.T. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Anim Reprod Sci 2002, 71:13-23.
22. Cemeli E., Baumgartner A., Anderson D. Antioxidants and the comet assay. Mutat Res 2009, 681:51-67.
23. Liu P.Y., Li K., Zhang J., Zhang D.W., Lin H.H., Yu X.Q. Who is the king? the alpha-hydroxy-beta-oxo-alpha,beta-enone moiety or the catechol B ring: relationship between the structure of quercetin derivatives and their pro-oxidative abilities. Chem Biodivers 2010, 7:236-244.
24. Cemeli E., Schmid T.E., Anderson D. Modulation by flavonoids of dna damage induced by estrogen-like compounds. Environ Mol Mutagen 2004, 44:420-426.
25. Córdoba M., Pintos L., Beconi M.T. Variations in creatine kinase activity and reactive oxygen species levels are involved in capacitation of bovine spermatozoa. Andrologia 2008, 40:370-376.
26. Bilodeau J.F., Blanchette S., Gagnon C., Sirard M.A. Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology 2001, 56:275-286.
27. Oeda T., Henkel R., Ohmori H., Schill W.B. Scavenging effect of N-acetyl-l-cysteine against reactive oxygen species in human semen: a possible therapeutic modality for male factor infertility?. Andrologia 1997, 29:125-131.
28. Michael A.J., Alexopoulos C., Pontiki E.A., Hadjipavlou-Litina D.J., Saratsis P., Ververidis H.N., et al. Effect of n-acetyl-l-cysteine supplementation in semen extenders on semen quality and reactive oxygen species of chilled canine spermatozoa. Reprod Domest Anim 2010, 45:201-207.
29. Pagl R., Aurich J.E., Müller-Schlösser F., Kankofer M., Aurich C. Comparison of an extender containing defined milk protein fractions with a skim milk-based extender for storage of equine semen at 5 degrees C. Theriogenology 2006, 66:1115-1122.
30. Martínez-Pastor F., Fernández-Santos M.R., del Olmo E., Domínguez-Rebolledo A.E., Esteso M.C., Montoro V., et al. Mitochondrial activity and forward scatter vary in necrotic, apoptotic and membrane-intact spermatozoan subpopulations. Reprod Fertil Dev 2008, 20:547-556.
31. Aitken R.J., Harkiss D., Buckingham D.W. Analysis of lipid peroxidation mechanisms in human spermatozoa. Mol Reprod Dev 1993, 35:302-315.
32. R: A language and environment for statistical computing 2011, R Development Core Team, R Foundation for Statistical Computing, Vienna, Austria.
33. Martinez-Pastor F., Garcia-Macias V., Alvarez M., Chamorro C., Herraez P., de Paz P., et al. Comparison of two methods for obtaining spermatozoa from the cauda epididymis of Iberian red deer. Theriogenology 2006, 65:471-485.
34. Aitken J., Fisher H. Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays 1994, 16:259-267.
35. Aitken R.J., Sawyer D. The human spermatozoon-not waving but drowning. Adv Exp Med Biol 2003, 518:85-98.
36. Bansal A.K., Bilaspuri G.S. Impacts of oxidative stress and antioxidants on semen functions. Vet Med Int 2010, Sep 7:1-7.
37. Donnelly E.T., McClure N., Lewis S.E. Glutathione and hypotaurine in vitro: effects on human sperm motility, DNA integrity and production of reactive oxygen species. Mutagenesis 2000, 15:61-68.
38. Ramos L., Wetzels A.M. Low rates of dna fragmentation in selected motile human spermatozoa assessed by the tunel assay. Hum Reprod 2001, 16:1703-1707.
39. Martínez-Pastor F., Aisen E., Fernández-Santos M.R., Esteso M.C., Maroto-Morales A., García-Alvarez O., et al. Reactive oxygen species generators affect quality parameters and apoptosis markers differently in red deer spermatozoa. Reproduction 2009, 137:225-235.
40. Martínez A.F., Martínez-Pastor F., Alvarez M., Fernández-Santos M.R., Esteso M.C., de Paz P., et al. Sperm parameters on Iberian red deer: electroejaculation and post-mortem collection. Theriogenology 2008, 70:216-226.
41. Varisli O., Uguz C., Agca C., Agca Y. Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of anisosmotic solutions, cryoprotective agents and low temperatures. Anim Reprod Sci 2009, 110:256-268.
42. Matás C., Sansegundo M., Ruiz S., García-Vázquez F.A., Gadea J., Romar R., et al. Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa. Theriogenology 2010, 74:1327-1340.
43. Krishna M.C., Russo A., Mitchell J.B., Goldstein S., Dafni H., Samuni A. Do nitroxide antioxidants act as scavengers of O2- or as SOD mimics?. J Biol Chem 1996, 271:26026-26031.
44. Chatterjee P.K., Cuzzocrea S., Brown P.A., Zacharowski K., Stewart K.N., Mota-Filipe H., et al. Tempol, a membrane-permeable radical scavenger, reduces oxidant stress-mediated renal dysfunction and injury in the rat. Kidney Int 2000, 58:658-673.
45. Baker M.A., Aitken R.J. The importance of redox regulated pathways in sperm cell biology. Mol Cell Endocrinol 2004, 216:47-54.
46. O'Flaherty C., de Lamirande E., Gagnon C. Reactive oxygen species modulate independent protein phosphorylation pathways during human sperm capacitation. Free Radic Biol Med 2006, 40:1045-1055.
47. de Lamirande E., O'Flaherty C. Sperm activation: role of reactive oxygen species and kinases. Biochim Biophys Acta 2008, 1784:106-115.
48. Liu G.A., Zheng R.L. Protection against damaged DNA in the single cell by polyphenols. Pharmazie 2002, 57:852-854.
49. Anderson D., Basaran N., Dobrzyńska M.M., Basaran A.A., Yu T.W. Modulating effects of flavonoids on food mutagens in human blood and sperm samples in the comet assay. Teratog Carcinog Mutagen 1997, 17:45-58.
50. Anderson D., Dobrzyńska M.M., Başaran N., Başaran A., Yu T.W. Flavonoids modulate comet assay responses to food mutagens in human lymphocytes and sperm. Mutat Res 1998, 402:269-277.
51. Utesch D., Feige K., Dasenbrock J., Broschard T.H., Harwood M., Danielewska-Nikiel B., et al. Evaluation of the potential in vivo genotoxicity of quercetin. Mutat Res 2008, 654:38-44.
52. Janjua N.K., Siddiqa A., Yaqub A., Sabahat S., Qureshi R., Ul Haque S. Spectrophotometric analysis of flavonoid-DNA binding interactions at physiological conditions. Spectrochim Acta A Molecular Biomol Spectrosc 2009, 74:1135-1137.
53. Hosseinimehr S.J. Flavonoids and genomic instability induced by ionizing radiation. Drug Discov Today 2010, 15:907-918.
54. Szeto Y.T., Collins A.R., Benzie I.F. Effects of dietary antioxidants on DNA damage in lysed cells using a modified comet assay procedure. Mutat Res 2002, 500:31-38.
55. 2+-stores in sperm: their identities and functions. Reprod 2009, 138:425-437.
56. Córdoba M., Pintos L., Beconi M.T. Differential activities of malate and isocitrate NAD(P)-dependent dehydrogenases are involved in the induction of capacitation and acrosome reaction in cryopreserved bovine spermatozoa. Andrologia 2005, 37:40-46.
57. 2+-transport ATPase from synaptosomal vesicles by flavonoids. Biochim Biophys Acta 1983, 730:245-254.
58. Deneke S.M. Thiol-based antioxidants. Curr Top Cell Regul 2000, 36:151-180.
59. Michael A., Alexopoulos C., Pontiki E., Hadjipavlou-Litina D., Saratsis P., Boscos C. Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology 2007, 68:204-212.
60. Grasa P., Colas C., Gallego M., Monteagudo L., Muiño-Blanco T., Cebrián-Pérez J.A. Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction. Reproduction 2009, 137:655-667.
61. Martínez-Pastor F., Mata-Campuzano M., Alvarez-Rodríguez M., Alvarez M., Anel L., de Paz P. Probes and techniques for sperm evaluation by flow cytometry. Reprod Domest Anim 2010, 45(Suppl 2):67-78.