Mitochondrial activity and forward scatter vary in necrotic, apoptotic and membrane-intact spermatozoan subpopulations

Reproduction, Fertility and Development

Volumn 20, Issue 5, 2008, Pages 547-556

  Martinez Pastor F ^a   Fernandez Santos M R ^a   Del Olmo, E ^a   Dominguez Rebolledo A E ^a   Esteso, M C ^a   Montoro, V ^a   Garde, J J ^a  

^a UNIVERSITY OF CASTILLA LA MANCHA   (Spain)

Author keywords

Cell volume; Flow cytometry; Membrane changes; Mitochondrial membrane potential; Sperm death; YO PRO 1

Indexed keywords

PROPIDIUM IODIDE; TROLOX C;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL SUBPOPULATION; CONTROLLED STUDY; FLOW CYTOMETRY; MALE; MITOCHONDRIAL MEMBRANE POTENTIAL; NECROSIS; NONHUMAN; OVIDUCT; SPERMATOZOON; SPERMATOZOON MOTILITY;

ANIMALS; ANTIOXIDANTS; APOPTOSIS; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; DEER; FLOW CYTOMETRY; LUMINESCENT PROTEINS; MALE; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; NECROSIS; SPERM COUNT; SPERM MOTILITY; SPERMATOZOA;

CERVUS ELAPHUS;

EID: 44649175717     PISSN: 10313613     EISSN: None     Source Type: Journal    
DOI: 10.1071/RD08002     Document Type: Article

References (40)

1. Aitken, R. J., Nixon, B., Lin, M., Koppers, A. J., Lee, Y. H., and Baker, M. A. (2007). Proteomic changes in mammalian spermatozoa during epididymal maturation. Asian J. Androl. 9, 554-564. doi:10.1111/J.1745-7262. 2007.00280.X
2. Aziz, N., Said, T., Paasch, U., and Agarwal, A. (2007). The relationship between human sperm apoptosis, morphology and the sperm deformity index. Hum. Reprod. 22, 1413-1419. doi:10.1093/HUMREP/DEM016
3. Ball, B. A., and Vo, A. (2002). Detection of lipid peroxidation in equine spermatozoa based upon the lipophilic fluorescent dye C11-BODIPY581/591. J. Androl. 23, 259-269.
4. Comizzoli, P., Mauget, R., and Mermillod, P. (2001). Assessment of in vitro fertility of deer spermatozoa by heterologous IVF with zona-free bovine oocytes. Theriogenology 56, 261-274. doi:10.1016/S0093- 691X(01)00561-1
5. de Vries, K. J., Wiedmer, T., Sims, P. J., and Gadella, B. M. (2003). Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells. Biol. Reprod. 68, 2122-2134. doi:10.1095/BIOLREPROD.102.012500
6. Fernandez-Santos, M. R., Martinez-Pastor, F., Garcia-Macias, V., Esteso, M. C., Soler, A. J., Paz, P., Anel, L., and Garde, J. J. (2007). Sperm characteristics and DNA integrity of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa frozen in the presence of enzymatic and nonenzymatic antioxidants. J. Androl. 28, 294-305. doi:10.2164/JANDROL.106.000935
7. Grizard, G., Ouchchane, L., Roddier, H., Artonne, C., Sion, B., Vasson, M. P., and Janny, L. (2007). In vitro alachlor effects on reactive oxygen species generation, motility patterns and apoptosis markers in human spermatozoa. Reprod.Toxicol. 23, 55-62. doi:10.1016/J.REPROTOX. 2006.08.007
8. Grunewald, S., Said, T. M., Paasch, U., Glander, H. J., and Agarwal, A. (2008). Relationship between sperm apoptosis signalling and oocyte penetration capacity. Int. J. Androl., in press. doi:10.1111/J.1365-2605.2007.00768.X
9. Hallap, T., Nagy, S., Jaakma, U., Johannisson, A., and Rodriguez-Martinez, H. (2005). Mitochondrial activity of frozen-thawed spermatozoa assessed by Mitotracker Deep Red 633. Theriogenology 63, 2311-2322. doi:10.1016/J.THERIOGENOLOGY.2004.10.010
10. Hallap, T., Nagy, S., Jaakma, U., Johannisson, A., and Rodriguez-Martinez, H. (2006). Usefulness of a triple fluorochrome combination merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls. Theriogenology 65, 1122-1136. doi:10.1016/J.THERIOGENOLOGY.2005. 07.009
11. 2, an effector of capacitation, induces a rapid and reversible change in the lipid architecture of boar sperm plasma membranes. Mol. Reprod. Dev. 45, 378-391. doi:10.1002/(SICI)1098-2795(199611) 45:3〈378::AIDMRD16〉3.0.CO;2-V
12. Idziorek, T., Estaquier, J., De Bels, F., and Ameisen, J. C. (1995). YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J. Immunol. Methods 185, 249-258. doi:10.1016/0022-1759(95)00172-7
13. Lemasters, J. J., Qian, T., Bradham, C. A., Brenner, D. A., Cascio, W. E., Trost, L. C., Nishimura, Y., Nieminen, A. L., and Herman, B. (1999). Mitochondrial dysfunction in the pathogenesis of necrotic and apoptotic cell death. J. Bioenerg. Biomembr. 31, 305-319. doi:10.1023/A: 1005419617371
14. Marin, S., Chiang, K., Bassilian, S., Lee, W. N. P., Boros, L. G., Fernandez-Novell, J. M., Centelles, J. J., Medrano, A., Rodriguez-Gil, J. E., and Cascante, M. (2003). Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization. FEBS Lett. 554, 342-346. doi:10.1016/S0014-5793(03)01185-2
15. Marti, E., Perez-Pe, R., Colas, C., Muino-Blanco, T., and Cebrian-Perez, J. A. (2008). Study of apoptosis-related markers in ram spermatozoa. Anim. Reprod. Sci., in press. doi:10.1016/J.ANIREPROSCI.2007.04.009.
16. Martin, G., Sabido, O., Durand, P., and Levy, R. (2005). Phosphatidylserine externalization in human sperm induced by calcium ionophore A23187: relationship with apoptosis, membrane scrambling and the acrosome reaction. Hum. Reprod. 20, 3459-3468. doi:10.1093/HUMREP/DEI245
17. Martin, G., Cagnon, N., Sabido, O., Sion, B., Grizard, G., Durand, P., and Levy, R. (2007). Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa. Hum. Reprod. 22, 380-388. doi:10.1093/HUMREP/DEL399
18. McGann, L. E., Walterson, M. L., and Hogg, L. M. (1988). Light scattering and cell volumes in osmotically stressed and frozen-thawed cells. Cytometry 9, 33-38. doi:10.1002/CYTO.990090106
19. Paasch, U., Grunewald, S., Fitzl, G., and Glander, H. J. (2003). Deterioration of plasma membrane is associated with activated caspases in human spermatozoa. J. Androl. 24, 246-252.
20. Paasch, U., Grunewald, S., Dathe, S., and Glander, H. J. (2004a). Mitochondria of human spermatozoa are preferentially susceptible to apoptosis. Ann. N. Y. Acad. Sci. 1030, 403-409. doi:10.1196/ANNALS.1329.050
21. Paasch, U., Sharma, R. K., Gupta, A. K., Grunewald, S., Mascha, E. J., Thomas, A. J. J., Glander, H. J., and Agarwal, A. (2004b). Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol. Reprod. 71, 1828-1837. doi:10.1095/BIOLREPROD.103.025627
22. Peña, F. J., Saravia, F., Johannisson, A., Walgren, M., and Rodriguez-Martinez, H. (2005). A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa. Int. J. Androl. 28, 107-114. doi:10.1111/J.1365-2605.2005.00512.X
23. Peña, F. J., Saravia, F., Johannisson, A., Wallgren, M., and Rodriguez-Martinez, H. (2007). Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa. Anim. Reprod. Sci. 97, 74-83. doi:10.1016/J.ANIREPROSCI.2005.12. 014
24. Petrunkina, A. M., and Topfer-Petersen, E. (2000). Heterogeneous osmotic behaviour in boar sperm populations and its relevance for detection of changes in plasma membrane. Reprod. Fertil. Dev. 12, 297-305. doi:10.1071/RD00087
25. Petrunkina, A. M., Harrison, R. A. P., Ekhlasi-Hundrieser, M., and Topfer-Petersen, E. (2004). Role of volume-stimulated osmolyte and anion channels in volume regulation by mammalian sperm. Mol. Hum. Reprod. 10, 815-823. doi:10.1093/MOLEHR/GAH106
26. Petrunkina, A. M., Gropper, B., Topfer-Petersen, E., and Gunzel-Apel, A. R. (2005a). Volume regulatory function and sperm membrane dynamics as parameters for evaluating cryoprotective efficiency of a freezing extender. Theriogenology 63, 1390-1406. doi:10.1016/J.THERIOGENOLOGY.2004. 07.006
27. Petrunkina, A. M., Jebe, E., and Topfer-Petersen, E. (2005b). Regulatory and necrotic volume increase in boar spermatozoa. J. Cell. Physiol. 204, 508-521. doi:10.1002/JCP.20317
28. Petrunkina, A. M., Waberski, D., Gunzel-Apel, A. R., and Topfer-Petersen, E. (2007). Determinants of sperm quality and fertility in domestic species. Reproduction 134, 3-17. doi:10.1530/REP-07-0046
29. R Development Core Team (2007). 'R: A Language and Environment for Statistical Computing.' (R Foundation for Statistical Computing: Vienna.)
30. Scherer, J. M., Stillwell, W., and Jenski, L. J. (1999). Anomalous changes in forward scatter of lymphocytes with loosely packed membranes. Cytometry 37, 184-190. doi:10.1002/(SICI)1097-0320(19991101)37: 3〉184::AID-CYTO4&;3.0.CO;2-X
31. Shapiro, H. M. (2003). 'Practical Flow Cytometry.' 4th Edn. (Wiley: Hoboken.)
32. - channel. Proc. Natl Acad. Sci. USA 101, 6770-6773. doi:10.1073/PNAS. 0401604101
33. Silva, P., and Gadella, B. (2006). Detection of damage in mammalian sperm cells. Theriogenology 65, 958-978. doi:10.1016/J.THERIOGENOLOGY. 2005.09.010
34. Soler, A. J., Garcia, A. J., Fernandez-Santos, M. R., Esteso, M. C., and Garde, J. J. (2003a). Effects of thawing procedure on postthawed in vitro viability and in vivo fertility of red deer epididymal spermatozoa cryopreserved at -196 degrees C. J. Androl. 24, 746-756.
35. Soler, A. J., Perez-Guzman, M. D., and Garde, J. J. (2003b). Storage of red deer epididymides for four days at 5 degrees C: effects on spermmotility, viability, and morphological integrity. J. Exp. Zoolog. A Comp. Exp. Biol. 295A, 188-199. doi:10.1002/JEZ.A.10194
36. Takahashi, Y., and First, N. L. (1992). In vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 37, 963-978. doi:10.1016/0093- 691X(92)90096-A
37. Taylor, S. L., Weng, S. L., Fox, P., Duran, E. H., Morshedi, M. S., Oehninger, S., and Beebe, S. J. (2004). Somatic cell apoptosis markers and pathways in human ejaculated sperm: potential utility as indicators of sperm quality. Mol. Hum. Reprod. 10, 825-834. doi:10.1093/MOLEHR/GAH099
38. Waterhouse, K. E., De Angelis, P. M., Haugan, T., Paulenz, H., Hofmo, P. O., and Farstad, W. (2004). Effects of in vitro storage time and semen-extender on membrane quality of boar sperm assessed by flow cytometry. Theriogenology 62, 1638-1651. doi:10.1016/J.THERIOGENOLOGY.2004. 03.001
39. Weil, M., Jacobson, M. D., and Raff, M. C. (1998). Are caspases involved in the death of cells with a transcriptionally inactive nucleus? Sperm and chicken erythrocytes. J. Cell Sci. 111, 2707-2715.
40. Yeung, C. H., Anapolski, M., and Cooper, T. G. (2002). Measurement of volume changes in mouse spermatozoa using an electronic sizing analyzer and a flow cytometer: Validation and application to an infertile mouse model. J. Androl. 23, 522-528.