Monitoring structural transitions in IDPs by site-directed spin labeling EPR spectroscopy

Methods in Molecular Biology

Volumn 895, Issue , 2012, Pages 361-386

  Habchi, Johnny ^a   Martinho, Marlène ^b   Gruet, Antoine ^a   Guigliarelli, Bruno ^b   Longhi, Sonia ^a   Belle, Valérie ^b  

^a AIX MARSEILLE UNIVERSITY   (France)

^b AIX MARSEILLE UNIVERSITÉ   (France)

Author keywords

Electron paramagnetic resonance spectroscopy; Induced folding; Intrinsically disordered proteins; MTSL; Nitroxide radical; SDSL EPR; Site directed spin labeling; Spin labels; Spin probes; Structural transitions

Indexed keywords

CYSTEINE; HYBRID PROTEIN; NITROGEN OXIDE; NITROXYL; SUCROSE; TRIFLUOROETHANOL;

AFFINITY CHROMATOGRAPHY; ALGORITHM; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; STAINING;

ALGORITHMS; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; CYSTEINE; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; MUTAGENESIS, SITE-DIRECTED; NITROGEN OXIDES; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING; SUCROSE; TRIFLUOROETHANOL;

EID: 84865332732     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-927-3_21     Document Type: Article

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