Molecular characterization reveals three distinct clonal groups among clinical shiga toxin-producing Escherichia coli strains of serogroup O103

Journal of Clinical Microbiology

Volumn 50, Issue 9, 2012, Pages 2894-2900

  Iguchi, Atsushi ^a   Iyoda, Sunao ^b   Ohnishi, Makoto ^b  

^a UNIVERSITY OF MIYAZAKI   (Japan)

^b NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

INTIMIN; SHIGA TOXIN;

ADK GENE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATION; EAE GENE; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTION; FLIC GENE; FUMC GENE; GENE ACTIVITY; GENE FUNCTION; GENE SEQUENCE; GENETIC CODE; GYRB GENE; ICD GENE; JAPAN; MDH GENE; MOLECULAR CLONING; MOLECULAR TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; PURA GENE; RECA GENE; SEQUENCE ANALYSIS; SEROTYPE;

ADHESINS, BACTERIAL; CLUSTER ANALYSIS; DNA, BACTERIAL; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; GENETIC VARIATION; HUMANS; JAPAN; MOLECULAR EPIDEMIOLOGY; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEQUENCE ANALYSIS, DNA; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 84865198604     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00789-12     Document Type: Article

References (44)

1. Beutin L, Kaulfuss S, Herold S, Oswald E, Schmidt H. 2005. Genetic analysis of enteropathogenic and enterohemorrhagic Escherichia coli sero-group O103 strains by molecular typing of virulence and housekeeping genes and pulsed-field gel electrophoresis. J. Clin. Microbiol. 43:1552-1563.
2. Beutin L, Krause G, Zimmermann S, Kaulfuss S, Gleier K. 2004. Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J. Clin. Microbiol. 42:1099-1108. (Pubitemid 38332832)
3. 2/M arrest, inhibition of proliferation, and death of human endothelial cells. Infect. Immun. 73:552-562.
4. Cebula TA, Payne WL, Feng P. 1995. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J. Clin. Microbiol. 33:248-250. (Pubitemid 24382441)
5. Cergole-Novella MC, et al. 2007. Distribution of virulence profiles related to new toxins and putative adhesins in Shiga toxin-producing Escherichia coli isolated from diverse sources in Brazil. FEMS Microbiol. Lett. 274:329-334. (Pubitemid 47256277)
6. Czeczulin JR, Whittam TS, Henderson IR, Navarro-Garcia F, Nataro JP. 1999. Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli. Infect. Immun. 67:2692-2699. (Pubitemid 29244680)
7. Eklund M, Scheutz F, Siitonen A. 2001. Clinical isolates of non-O157 Shiga toxin-producing Escherichia coli: serotypes, virulence characteristics, and molecular profiles of strains of the same serotype. J. Clin. Microbiol. 39:2829-2834. (Pubitemid 32743997)
8. Fields PI, et al. 1997. Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM. J. Clin. Microbiol. 35:1066-1070. (Pubitemid 27175226)
9. Gunzburg ST, Tornieporth NG, Riley LW. 1995. Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene. J. Clin. Microbiol. 33:1375-1377.
10. Hayashi T, et al. 2001. Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res. 8:11-22. (Pubitemid 33617542)
11. Hyma KE, et al. 2005. Evolutionary genetics of a new pathogenic Escherichia species: Escherichia albertii and related Shigella boydii strains. J. Bacteriol. 187:619-628. (Pubitemid 40096249)
12. Iguchi A, Ooka T, Ogura Y, Asadulghani Nakayama K, Frankel G, Hayashi T. 2008. Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli O55 strains belonging to three distinct lineages. Microbiology 154:559-570. (Pubitemid 351292310)
13. Iguchi A, et al. 2011. Wide distribution of O157-antigen biosynthesis gene clusters in Escherichia coli. PLoS One 6:e23250. doi:10.1371/journal.pone. 0023250.
14. Kappeli U, Hachler H, Giezendanner N, Beutin L, Stephan R. 2011. Human infections with non-O157 Shiga toxin-producing Escherichia coli, Switzerland, 2000-2009. Emerg. Infect. Dis. 17:180-185.
15. L'Abee-Lund TM, et al. 2012. The highly virulent 2006 Norwegian EHEC O103:H25 outbreak strain is related to the 2011 German O104:H4 outbreak strain. PLoS One 7:e31413. doi:10.1371/journal.pone.0031413.
16. Lathrop S, Edge K, Bareta J. 2009. Shiga toxin-producing Escherichia coli, New Mexico, USA, 2004-2007. Emerg. Infect. Dis. 15:1289-1291.
17. Leopold SR, et al. 2009. A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis. Proc. Natl. Acad. Sci. U. S. A. 106:8713-8718.
18. Madic J, et al. 2010. Simplex and multiplex real-time PCR assays for the detection of flagellar (H-antigen) fliC alleles and intimin (eae) variants associated with enterohaemorrhagic Escherichia coli (EHEC) serotypes O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7. J. Appl. Microbiol. 109:1696-1705.
19. Mariani-Kurkdjian P, et al. 1993. Identification of a clone of Escherichia coli O103:H2 as a potential agent of hemolytic-uremic syndrome in France. J. Clin. Microbiol. 31:296-301. (Pubitemid 23028628)
20. Muraoka R, et al. 2007. An enterohemorrhagic Escherichia coli O103 outbreak at a nursery school in Miyazaki Prefecture, Japan. Jpn. J. Infect. Dis. 60:410-411. (Pubitemid 350177156)
21. Nataro JP, Kaper JB. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142-201. (Pubitemid 28046019)
22. Newton HJ, et al. 2009. Shiga toxin-producing Escherichia coli strains negative for locus of enterocyte effacement. Emerg. Infect. Dis. 15:372-380.
23. Ogura Y, et al. 2007. Extensive genomic diversity and selective conservation of virulence-determinants in enterohemorrhagic Escherichia coli strains of O157 and non-O157 serotypes. Genome Biol. 8:R138.
24. Ogura Y, et al. 2009. Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 106:17939-17944.
25. Ooka T, et al. 2012. Clinical Significance of Escherichia albertii. Emerg. Infect. Dis. 18:488-492.
26. Oswald E, et al. 2000. Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect. Immun. 68:64-71. (Pubitemid 30016678)
27. 2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J. Clin. Microbiol. 36:598-602.
28. Paton AW, Paton JC. 2002. Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J. Clin. Microbiol. 40:271-274. (Pubitemid 34027638)
29. Ramos-Moreno AC, Cabilio-Guth BE, Baquerizo-Martinez M. 2006. Can. the fliC PCR-restriction fragment length polymorphism technique replace classic serotyping methods for characterizing the H antigen of enterotoxigenic Escherichia coli strains? J. Clin. Microbiol. 44:1453-1458.
30. Ramotar K, Henderson E, Szumski R, Louie TJ. 1995. Impact of free verotoxin testing on epidemiology of diarrhea caused by verotoxin-producing Escherichia coli. J. Clin. Microbiol. 33:1114-1120.
31. Rivas M, et al. 2006. Characterization and epidemiologic subtyping of Shiga toxin-producing Escherichia coli strains isolated from hemolyticuremic syndrome and diarrhea cases in Argentina. Foodborne Pathog. Dis. 3:88-96.
32. Rivas M, et al. 2008. Risk factors for sporadic Shiga toxin-producing Escherichia coli infections in children, Argentina. Emerg. Infect. Dis. 14:763-771. (Pubitemid 351838640)
33. Rohde H, et al. 2011. Open-source genomic analysis of Shiga-toxin-producing Escherichia coli O104:H4. N. Engl. J. Med. 365:718-724.
34. Saito S, et al. 1998. A familial outbreak of verotoxin-producing Escherichia coli O103:H2 infection in which a calf was the suspected infectious source. Kansenshogaku Zasshi. 72:707-713. (In Japanese.)
35. Schimmer B, et al. 2008. Outbreak of haemolytic uraemic syndrome in Norway caused by stx2-positive Escherichia coli O103:H25 traced to cured mutton sausages. BMC Infect. Dis. 8:41. doi:10.1186/1471-2334-8-41.
36. Sethabutr O, et al. 2000. Detection of PCR products of the ipaH gene from Shigella and enteroinvasive Escherichia coli by enzyme-linked immunosorbent assay. Diagn. Microbiol. Infect. Dis. 37:11-16. (Pubitemid 30224517)
37. Seto K, Taguchi M, Kobayashi K, Kozaki S. 2007. Biochemical and molecular characterization of minor serogroups of Shiga toxin-producing Escherichia coli isolated from humans in Osaka prefecture. J. Vet. Med. Sci. 69:1215-1222.
38. Spika JS, Michel P, Milley D, Wilson J, Waters J. 1998. Shiga toxin-producing Escherichia coli infections in Canada, p 23-29. In Kaper JB, O'Brien AD (ed), Escherichia coli O157:H7 and other Shiga-producing E. coli strains. ASM Press, Washington, DC.
39. Stacy-Phipps S, Mecca JJ, Weiss JB. 1995. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. J. Clin. Microbiol. 33:1054-1059.
40. Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599. (Pubitemid 47236692)
41. Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680. (Pubitemid 24354800)
42. Thompson LH, Giercke S, Beaudoin C, Woodward D, Wylie JL. 2005. Enhanced surveillance of non-O157 verotoxin-producing Escherichia coli in human stool samples from Manitoba. Can. J. Infect. Dis. Med. Microbiol. 16:329-334. (Pubitemid 43089176)
43. Wick LM, Qi W, Lacher DW, Whittam TS. 2005. Evolution of genomic content in the stepwise emergence of Escherichia coli O157:H7. J. Bacteriol. 187:1783-1791. (Pubitemid 40289380)
44. Yamamoto T, Echeverria P. 1996. Detection of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene sequences in enterotoxigenic E. coli strains pathogenic for humans. Infect. Immun. 64:1441-1445. (Pubitemid 26102415)