Semen Evaluation

Equine Breeding Management and Artificial Insemination

Volumn , Issue , 2009, Pages 57-74

  Sieme, Harald ^a  

^a University Veterinary Medicine Foundation   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84865182579     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-1-4160-5234-0.00006-4     Document Type: Chapter

References (235)

1. Colenbrander B., Puyk H., Zandee A.R., et al. Evaluation of the stallion for breeding. Acta Vet Scand Suppl 1992, 88:29.
2. Jasko DJ: Evaluation of stallion semen. In Blanchard TL, Varner DD, eds: Vet Clin North Am Eq Pract 8:129, 1992.
3. Amann R.P., Hammerstedt R.H. In vitro evaluation of sperm quality: an opinion. J Androl 1993, 14:397.
4. Malmgren L. Assessing the quality of raw semen: a review. Theriogenology 1997, 48:523.
5. Magistrini M., Guitton E., Le Vern Y., et al. New staining methods for sperm evaluation estimated by microscopy and flow cytometry. Theriogenology 1997, 48:1229.
6. Juhasz J., Nagy P., Kulcsar M. Method for semen and endocrinological evaluation of the stallion: a review. Acta Vet Brno 2000, 69:247.
7. Katila T. In vitro evaluation of frozen-thawed stallion semen: a review. Acta Vet Scand 2001, 42:199.
8. Card C. Cellular associations and the differential spermiogram: making sense of stallion spermatozoal morphology. Theriogenology 2005, 64:558.
9. Graham J.K., Mocé E. Fertility evaluation of frozen/thawed semen. Theriogenology 2005, 64:492.
10. Petrunkina A.M., Waberski D., Günzel-Apel A.R., et al. Determinants of sperm quality and fertility in domestic species. Reproduction 2007, 134:3.
11. Klug E: Untersuchungen zur klinischen Andrologie des Pferdes. Die Bedeutung andrologischer Befunde am Hengst für den Zuchteinsatz, Hannover, Tierärztl Hochsch, Habil-Schr, 1982.
12. Parlevliet J.M., Kemp B., Colenbrander B. Reproductive characteristics and semen quality in maiden Dutch Warmblood stallion. J Reprod Fertil 1994, 101:183.
13. Dowsett K.F., Pattie W.A. Variation in characteristics of stallion semen caused by breed, age and season of year and service frequency. J Reprod Fertil 1987, 35:45.
14. Pickett B.W., Voss J.L., Bowen R.A., et al. Seminal characteristics and total scrotal width (T.S.W.) of normal and abnormal stallions. Proc AAEP 1988, 485.
15. Paccamonti D.L., Buiten A.V., Parlevliet J.M., et al. Reproductive parameters of miniature stallions. Theriogenology 1999, 51:1343.
16. Bielanski W., Kaczmarski F. Morphology of spermatozoa in semen from stallions of normal fertility. J Reprod Fertil Suppl 1979, 27:39.
17. Bielanski W., Dudek E., Bittmar A., et al. Some characteristics of common abnormal forms of spermatozoa in highly fertile stallions. J Reprod Fertil Suppl 1982, 32:21.
18. Voss J.L., Pickett B.W., Squires E.L. Stallion spermatozoal morphology and motility and their relationship to fertility. J Am Vet Med Assoc 1981, 178:287.
19. Voss J.L., Pickett B.W., Loomis P.R. The relationship between seminal characteristics and fertility in Thoroughbred stallions. J Reprod Fertil Suppl 1982, 32:635.
20. Dowsett K.F., Pattie W.A. Characteristics and fertility of stallion semen. J Reprod Fertil Suppl 1982, 32:1.
21. Amann R.P. Can the fertility potential of a seminal sample be predicted accurately?. J Andrology 1989, 10:89.
22. Jasko D.J., Lein D.H., Foote R.H. Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988). J Am Vet Med Assoc 1990, 197:389.
23. Jasko D.J., Little T.V., Lein D.H., et al. Comparison of spermatozoal movement and semen characteristics with fertility in stallions: 64 cases (1987-1988). J Am Vet Med Assoc 1992, 200:979.
24. Graham J.K. Analysis of stallion semen and its relation to fertility. Vet Clin Eq Pract 1996, 12:119.
25. Rodriguez-Martinez H. Laboratory semen assessment and prediction of fertility: still Utopia?. Reprod Domest Anim 2003, 38:312.
26. Rodriguez-Martinez H. Can we increase the estimative value of semen assessment?. Reprod Domest Anim Suppl 2006, 41:2.
27. Colenbrander B., Gadella B.M., Stout T.A.E. The predictive value of semen analysis in the evaluation of stallion fertility. Reprod Domest Anim 2003, 38:305.
28. Kirk E.S., Squires E.L., Graham J.K. Comparison of in vitro laboratory analyses with the fertility of cryopreserved stallion spermatozoa. Theriogenology 2005, 64:1422.
29. Kuisma P., Andersson M., Koskinen E., et al. Fertility of frozen-thawed stallion semen cannot be predicted by the currently used laboratory methods. Acta Vet Scand 2006, 48:14.
30. Magistrini M., Vidament M., Clement F., et al. Fertility prediction in stallions. Anim Reprod Sci 1996, 42:181.
31. Amann R.P. Weaknesses in reports of "fertility" for horses and other species. Theriogenology 2005, 63:698.
32. Pickett B.W., Squires E.L., McKinnon A.O. Procedures for collection, evaluation and utilization of stallion semen for artificial insemination 1987, Anim Reprod Lab Colorado State University, Fort Collins, CO.
33. Parlevliet J.M., Colenbrander B.C. Prediction of first season stallion fertility of 3-year-old Dutch Warmbloods with prebreeding assessment of percentage of morphologically normal live sperm. Eq Vet J 1999, 31:248.
34. Varner D.D., Blanchard T.L., Brinsko S.P., et al. Techniques for evaluating selected reproductive disorders of stallions. Anim Reprod Sci 2000, 60-61:493.
35. Silva P.F., Gadella B.M. Detection of damage in mammalian sperm cells. Theriogenology 2006, 65:958.
36. Love C.C., Varner D.D., Thompson J.A. Intra- and inter-stallion variation in sperm morphology and their relationship with fertility. J Reprod Fertil Suppl 2000, 56:93.
37. Clement F., Ladonnet Y., Magistrini M. Sperm morphology and fertility. Anim Reprod Sci 2001, 68:362.
38. Veeramachaneni D.N., Moeller C.L., Sawyer H.R. Sperm morphology in stallions: ultrastructure as a functional and diagnostic tool. Vet Clin Eq 2006, 22:683.
39. Brito F. Evaluation of stallion sperm morphology. Clin Tech Eq Pract 2007, 6:249.
40. Vidament M., Magistrini M., Palmer E., et al. Equine artificial insemination in French National Studs. Reprod Dom Anim Suppl 1999, 6:61.
41. Amann R.P., Cristanelli M.J., Squires E.L. Proteins in stallion seminal plasma. J Reprod Fertil Suppl 1987, 35:113.
42. Calvete J.J., Nessau S., Mann K., et al. Isolation and biochemical characterization of stallion seminal-plasma proteins. Reprod Dom Anim 1994, 29:411.
43. Töpfer-Petersen E., Ekhlasi-Hundtrieser M., Kirchhoff C., et al. The role of stallion seminal proteins in fertilisation. Anim Reprod Sci 2005, 89:159.
44. Sieme H., Katila T., Klug E. Effect of semen collection practices on sperm characteristics before and after storage and on fertility of stallions. Theriogenology 2004, 61:769.
45. Janett F., Thun R., Niederer K., et al. Seasonal changes of semen quality and freezability in the Warmblood stallion. Theriogenology 2003, 60:453.
46. Turner R.M. Current techniques for evaluation of stallion fertility. Clin Tech Equine Pract 2005, 4:257.
47. Tomlinson M., Turner J., Powell G., et al. One-step disposable chambers for sperm cell concentration and motility assessment: how do they compare with the World Health Organization's recommended methods. Human Reprod 2001, 16:121.
48. Bailey E., Fenning N., Chamberlain S., et al. Validation of sperm counting methods using limits of agreement. J Andrology 2007, 28:364.
49. Christensen P., Stryhn H., Hansen C. Discrepancies in the determination of sperm concentration using Bürker-Türk. Thoma and Makler counting chambers. Theriogenology 2005, 63:992.
50. Vanderwall D.K. Counting spermatozoa with a hemacytometer. J Equine Vet Sci 2008, 28:244.
51. Evenson D.P., Parks J.P., Kaproth M.T., et al. Rapid determination on sperm cell concentration in bovine semen by flow cytometry. J Dairy Sci 1993, 76:86.
52. Hansen C., Vermeiden T., Vermeiden J.P.W., et al. Comparison of FACSCount AF system, improved Neubauer hemacytometer, Corning 254 photometer, SpermVision, UltiMate and NucleoCounter SP-100 for determination of sperm concentration of boar semen. Theriogenology 2006, 66:2188.
53. Amann R.P., Katz D.F. Reflections on CASA after 25 years. J Androl 2004, 25:317.
54. Varner D.D., Vaughan S.D., Johnson L. Use of a computerized system for evaluation of equine spermatozoal motility. Am J Vet Res 1991, 52:224.
55. Jasko D.J., Lein D.H., Foote R.H. A comparison of two computer-automated semen analysis instruments for the evaluation of sperm motion characteristics in the stallion. J Andr 1990, 11:453.
56. Palmer E., Magistrini M. Automated analysis of stallion semen post-thaw motility. Acta Vet Scand Suppl 1992, 88:137.
57. Davis R.O., Katz D.F. Operational standards for CASA instruments. J Androl 1993, 14:385.
58. Wessel M.T., Althouse G.C. Validation of an objective approach for simultaneous assessment of viability and motility of fresh and cooled equine spermatozoa. Anim Reprod Sci 2006, 94:21.
59. Bataille B, Magistrini M, Palmer E: Objective determination of sperm motility in frozen-thawed stallion semen. Correlation with fertility. 16th Journée d́étude du CEREOPA, Paris, France. Anim Breed Abstr, 1990, pp 96-106.
60. Burns P.J., Reasner D.S. Computerized analysis of sperm motion: effects of glycerol concentration on the cryopreservation of equine spermatozoa. J Equine Vet Sci 1995, 15:377.
61. Samper J.C., Hellander J.C., Crabo B.G. Relationship between the fertility of fresh and frozen stallion semen and semen quality. J Reprod Fertil Suppl 1991, 44:107.
62. Wilhelm K.M., Graham J.K., Squires E.L. Comparison of the fertility of cryopreserved stallion spermatozoa with sperm motion analyses, flow cytometric evaluation, and zona-free hamster oocyte penetration. Theriogenology 1996, 46:559.
63. Evenson D.P., Darzynkiewicz Z., Melamed M.R. Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J Histochem Cytochem 1982, 30:279.
64. Kavak A., Johannisson A., Lundeheim N., et al. Evaluation of cryopreserved stallion semen from Tori and Estonian breeds using CASA and flow cytometry. Anim Reprod Sci 2003, 76:205.
65. Love C.C., Thompson J.A., Brinsko S.P., et al. Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry. Theriogenology 2003, 60:1127.
66. Quintero-Moreno A., Miró J., Teresa Rigau A., et al. Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates. Theriogenology 1973, 59:2003.
67. Tischner M. Evaluation of deep-frozen semen in stallions. J Reprod Fertil Suppl 1979, 27:53.
68. Müller Z. Practicalities of insemination of mares with deep-frozen semen. J Reprod Fertil Suppl 1987, 35:121.
69. Bittmar A., Kosiniak K. The role of selected biochemical components of equine seminal plasma in determining suitability for deep-freezing. Arch Vet Pol 1992, 32:17.
70. Samper J.C., Morris C.A. Current methods for stallion semen cryopreservation: a survey. Theriogenology 1998, 49:895.
71. Vidament M. French field results (1985-2005) on factors affecting fertility of frozen stallion semen. Anim Reprod Sci 2005, 89:115.
72. Loomis P.R. Artificial insemination of horses: where is it going? 1999, Proc Ann Conf Soc Theriogenol, Nashville, TN, pp 325-336.
73. Loomis P.R., Graham J.K. Commercial semen freezing: Individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Anim Reprod Sci 2008, 105:119.
74. Davis R.O., Gravance C.G. Standardization of specimen preparation, staining, and sampling methods improves automated sperm-head morphometry analysis. Fertil Steril 1993, 59:412.
75. Ball B.A., Mohammed H.O. Morphometry of stallion spermatozoa by computer assisted image analysis. Theriogenology 1995, 44:367.
76. Gravance C.G., Liu I.K., Davis R.O., et al. Quantification of normal head morphometry of stallion spermatozoa. J Reprod Fertil 1996, 108:41.
77. Card C. Detection of abnormal stallion sperm cells by using the Feulgen stain. Proc AAEP Denver, 1998, 44:9.
78. Casey P.I., Gravance C.G., Davis R.O., et al. Morphometric differences in sperm head dimensions of fertile and subfertile stallions. Theriogenology 1997, 47:575.
79. Gravance C.G., Champion Z., Liu I.K., et al. Sperm head morphometry analysis of ejaculate and dismount stallion semen samples. Anim Reprod Sci 1997, 47:149.
80. Dott H.M. Morphology of stallion spermatozoa. J Reprod Fertil Suppl 1975, 23:41.
81. Veeramachaneni D.N., Sawyer H.R. Use of semen as biopsy material for assessment of health status of the stallion reproductive tract. Vet Clin North Am Equine Pract 1996, 12:101.
82. Williams W.W. Technique of collecting semen for laboratory examination with a review of several diseased bulls. Cornell Vet 1920, 10:87.
83. Karras W. Spermastudien 1. Mitteillung: Eine Methode zur färeberischen Darstellung der Kopfkappen und des Kolloidüberzuges der Spermien. Monatshefte Prakt Tierhlkd 1950, 2:162.
84. Schaaf A. Vitalkleuring van stieren sperma met een oplossing van anilineblauw en eosine. Tijdschr Diergeneesk 1952, 77:815.
85. Dott H.M., Foster G.C. A technique for studying the morphology of mammalian spermatozoa which is eosinophilic in a differential "live-dead" stain. J Reprod Fertil 1972, 29:443.
86. Oettle E.E. Using a new acrosome stain to evaluate sperm morphology. Vet Med 1986, 81:263.
87. Hancock J.L., Hovell G.J.R. The collection of boar semen. Vet Rec 1959, 71:664.
88. Lagerlöf N. Morphological studies on the change in sperm structure and in the testes of bulls with decreased or abolished fertility. Acta Pathol Microbiol Scand Suppl 1934, 19:254.
89. Casserett G.W. One solution stain for spermatozoa. Stain Technol 1953, 22:125.
90. Hancock J.L. The morphology of boar sperm. J R Micro Soc 1957, 76:84.
91. Pesch S., Bostedt H., Failing K., et al. Advanced fertility diagnosis in stallion semen using transmission electron microscopy. Anim Reprod Sci 2006, 91:285.
92. Kenney R.M., Hurtgen J., Pierson R., et al. Theriogenology and the equine, part II: The Stallion (Society for Theriogenology Manual for Clinical Fertility Evaluation of the Stallion). J Soc Theriog 1983, 9:1100.
93. Bielanski W. Characteristics of the semen of stallions. Macro- and microscopic investigations with estimation of fertility. Mém Acad Polon Sci Lettr. Cl Sci Math Nat 1951, 16:59.
94. Barth A., Oko A. Evaluation of the spermiogram, abnormal morphology of bull spermatozoa 1989, Iowa State University Press, Ames, IA, pp 271-279.
95. Varner D.D. Introduction of the stallion breeding soundness examination form of the Society for Theriogenology 2002, Proc Soc Theriogenology, San Antonio, TX, pp 113-116.
96. Blom E. Interpretation of spermatic cytology in bulls. Fertil Steril 1950, 1:223.
97. Blom E. The ultrastructure of some characteristic sperm defects and a proposal for a new classification of the bull spermiogram. Nord Vet Med 1973, 25:383.
98. Saacke R.G., Dalton J.C., Nadir S., et al. Relationship of seminal traits and insemination time to fertilization rate and embryo quality. Anim Reprod Sci 2000, 60-61:663.
99. Love CC: Stallion semen evaluation and interpretation. Proc Soc Theriogenology. Colorado Springs, CO: 2002, pp 93-102.
100. Morrell J.M. Applications of flow cytometry to artificial insemination: a review. Vet Rec 1991, 129:375.
101. Gadella B.M., Rathi R., Brouwers J.F., et al. Capacitation and the acrosome reaction in equine spermatozoa. Anim Reprod Sci 2001, 68:249.
102. Caiza de la Cueva F.I., Pujol M.R., Rigau T., et al. Resistance to osmotic stress of horse spermatozoa: The role of ionic pumps and their relationship to cryopreservation success. Theriogenology 1997, 48:947.
103. Garner D.L., Pinkel D., Johnson L.A., et al. Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analyses. Biol Reprod 1986, 34:127.
104. Harrison R.A.P., Vickers S.E. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil 1990, 88:343.
105. Harkema W, Boyle MS: Use of fluorescent stains to assess membrane integrity of equine spermatozoa. Proc 12th Intern Congr Anim Reprod. The Hague, The Netherlands: 3:1424, 1992.
106. Garner D.L., Johnson L.A. Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 1995, 53:276.
107. Merkies K., Chenier T., Plante C., et al. Assessment of stallion permatozoa viability by flow cytometry and light microscope analysis. Theriogenology 2000, 54:1215.
108. Garner D.L., Thomas C.A., Joerg H.W., et al. Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol Reprod 1997, 57:1401.
109. Garner D.L., Thomas C.A. Organelle-specific probe JC-1 identifies membrane potential differences in the mitochondrial function of bovine sperm. Mol Reprod Dev 1999, 53:222.
110. Gravance C.G., Garner D.L., Baumber J., et al. Assessment of equine sperm mitochondrial function using JC-1. Theriogenology 2000, 53:1691.
111. Cross N.L., Meizel S. Methods for evaluating the acrosomal status of mammalian sperm. Biol Reprod 1989, 41:635.
112. Jankovicová J., Simon M., Antalíková J. Horovská L: Acrosomal and viability status of bovine spermatozoa evaluated by two staining methods. Acta Vet Hung 2008, 56:133.
113. Farlin M.E., Iasko Dl., Graham I.K., et al. Assessment of Pisum sativum agglutinin in identifying acrosomal damage in stallion spermatozoa. Mol Reprod Dev 1992, 32:23.
114. Casey P.J., Hillman R.B., Robertson K.R., et al. Validation of an acrosomal stain for equine sperm that differentiates between living and dead sperm. J Androl 1993, 14:289.
115. Cheng F.P., Fazeli A., Voorhout W.F., et al. Use of PNA (peanut agglutinin) to assess the acrosomal status and the zona pellucida induced acrosome reaction in stallion spermatozoa. J Andr 1996, 17:674.
116. Blottner S., Wegner H., Roelants H., et al. Flow cytometric determination of the acrosomal status of bull and stallion spermatozoa after marking with FITC-conjugated PNA (peanut agglutinin). Tierärztl Umsch 1998, 53:442.
117. Arruda R.P., Souza N.L., Marques A., et al. Evaluation of techniques using CFDA/PI, H258/FITC-PSA and Trypan blue/Giemsa for assessment of the viability and acrosomal integrity of cryopreserved equine spermatozoa. Theriogenology 2002, 57:477.
118. Bosard T.S., Love C.C., Brinsko S.P., et al. Evaluation and diagnosis of acrosome function/dysfunction in the stallion. Anim Reprod Sci 2005, 89:215.
119. Brum A.M., Thomas A.D., Sabeur K., et al. Evaluation of Coomassie blue staining of the acrosome of equine and canine spermatozoa. Am J Vet Res 2006, 67:358.
120. Ward C.R., Storey B.T. Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay. Dev Biol 1984, 104:287.
121. Varner D.D., Ward C.R., Storey B.A., et al. Induction and characterization of acrosome reaction in equine spermatozoa. Am J Vet Res 1987, 48:1383.
122. Rathi R., Colenbrander B., Bevers M.M., et al. Evaluation of in vitro capacitation of stallion spermatozoa. Biol Reprod 2001, 65:462.
123. Landim-Alvarenga F.C., Graham J.K., Alvarenga M.A., et al. Calcium influx into equine and bovine spermatozoa during in vitro capacitation. Anim Reprod 2004, 1:96.
124. Evenson D.P. Flow cytometric analysis of male germ cell quality. Methods Cell Biol 1990, 33:401.
125. Evenson D., Jost L. Sperm chromatin structure assay is useful for fertility assessment. Methods Cell Sci 2000, 22:169.
126. Love C.C. The sperm chromatin structure assay: a review of clinical applications. Anim Reprod Sci 2005, 89:39.
127. Cunningham R.E. Flow cytometry. Molecular Biomethods Handbook 1998, 653-667. Humana Press, Totowa, NJ. R. Rapley, J.M. Walker (Eds.).
128. Gillan L., Evans G., Maxwell W.M.C. Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 2005, 63:445.
129. Graham J.K. Assessment of sperm quality: a flow cytometric approach. Anim Reprod Sci 2001, 68:239.
130. Aurich J.E., Kühne A., Hoppe H., et al. Seminal plasma affects membrane integrity and motility of equine spermatozoa after cryopreservation. Theriogenology 1996, 46:791.
131. Aurich J.E., Schönherr U., Hoppe H., et al. Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen. Theriogenology 1997, 48:185.
132. Pintado B., de la Fuente J., Roldan E.R. Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechst 33258, or to eosin: accuracy in the assessment of cell viability. J Reprod Fertil 2000, 118:145.
133. Thomas C.A., Garner D.L., DeJarnette J.M., et al. Fluorometric assessments of acrosomal integrity and viability in cryopreserved bovine spermatozoa. Biol Reprod 1997, 56:991.
134. Jeyendran R.S., Van der Ven H.H., Perez-Palaez M., et al. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984, 70:219.
135. Jeyendran R.S., Van der Ven H.H., Zaneveld L.I.D. The hypoosmotic swelling test: an update. Arch Androl 1992, 29:105.
136. Vidament M., Cognard E., Yvon J.-M., et al. Evaluation of stallion semen before and after freezing. Reprod Dom Anim 1998, 33:271.
137. Neild D., Chaves G., Flores M., et al. Hypoosmotic test in equine spermatozoa. Theriogenology 1999, 51:721.
138. Neild D.C., Chaves M.G., Flores M., et al. The HOS test and its relationship to fertility in the stallion. Andrologia 2000, 32:351.
139. Katila T., Koskinen E., Andersson M. Evaluation of frozen-thawed semen. Havemeyer Foundation Workshop Advanced Current Topics in Stallion Veterinary Practice Krakow 2000, 54-56.
140. Petrunkina A.M., Petzoldt R., Stahlberg S., et al. Sperm-cell volumetric measurements as parameters in bull semen function evaluation: correlation with nonreturn rate. Andrologia 2001, 33:360.
141. Petrunkina A.M., Harrison R.A.P., Tsolova M., et al. Signalling pathways involved in the control of spermatozoal cell volume. Reproduction 2007, 133:61.
142. Flesch F.M., Gadella B.M. Dynamics of the mammalian sperm plasma membrane in the process of fertilization. Biochem Biophys Acta 2000, 1469:197.
143. Neild D.M., Gadella B.M., Agüero A., et al. Capacitation, acrosome function and chromatin structure in stallion sperm. Anim Reprod Sci 2005, 89:47.
144. Hunter R.H., Rodriguez-Martinez H. Capacitation of mammalian spermatozoa in vivo, with a specific focus on events in the fallopian tubes. Mol Reprod Dev 2004, 67:243.
145. Brewis I.A., Moore H.D., Fraser L.R., et al. Molecular mechanisms during sperm capacitation. Human Fertil 2005, 8:253.
146. Neild D.M., Gadella B.M., Chaves M.G., et al. Membrane changes during different stages of a freeze/thaw protocol for equine semen cryopreservation. Theriogenology 2003, 59:1693.
147. Magistrini M., Palmer E. Motility, triple stain and electron microscopic analysis of spermatozoa treated with ionophore A23187 for in vitro fertilization. J Reprod Fertil Suppl 1991, 44:661.
148. Mollova M., Atanassov B., Nedkova R., et al. Biochemical and immunochemical characterization of boar sperm flagellar protein with role in hyperactivation/capacitation process. Reprod Biol 2006, 6:79.
149. Pommer A.C., Rutlant J., Meyers S.A. Phosphorylation of protein tyrosine residues in fresh and cryopreserved stallion spermatozoa under capacitating conditions. Biol Reprod 2003, 68:1208.
150. Rathi R., Colenbrander B., Stout T.A.E., et al. Progesterone induces acrosome reaction in stallion spermatozoa via a protein tyrosine kinase dependent pathway. Mol Reprod Dev 2003, 64:120.
151. Piehler E., Petrunkina A.M., Ekhlasi-Hundrieser M., et al. Dynamic quantification of the tyrosine phosphorylation of the sperm surface proteins during capacitation. Cytometry 2006, 69:1062.
152. Flesch F.M., Brouwers J.F., Nievelstein P.F., et al. Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane. J Cell Sci 2001, 114:3543.
153. Visconti P.E., Ning X.P., Fornes M.W., et al. Cholesterol efflux mediated signal transduction in mammalian sperm: cholesterol release signals an increase in protein tyrosine phosphorylation during mouse sperm capacitation. Dev Biol 1999, 214:429.
154. Pena A.I., Lugilde L.L., Barrio M., et al. Effects of Equex from different sources on post-thaw survival, longevity and intracellular Ca2+ of dog spermatozoa. Theriogenology 2003, 59:1725.
155. Harrison R.A.P., Gadella B.M. Bicarbonate-induced membrane processing in sperm capacitation. Theriogenology 2005, 63:342.
156. Gadella B.M., Miller N.G.A., Colenbrander B., et al. Flow cytometric detection of transbilayer movement of fluorescent phospholipid analogues across the boar sperm plasma membrane: elimination of labelling artefacts. Mol Reprod Dev 1999, 53:108.
157. Cheng F.P., Fazeli A., Voorhout W.F., et al. Progesterone in mare follicular fluid induces the acrosome reaction in stallion spermatozoa and enhances in vitro binding to the zona pellucida. Int J Androl 1998, 21:57.
158. Sinowitz F., Wessa E., Neumüller C., et al. On the species specificity of sperm binding and sperm penetration of the zona pellucida. Reprod Domest Anim 2003, 38:141.
159. Blottner S., Warnke C., Tuchscherer A., et al. Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season. Anim Reprod Sci 2001, 65:75.
160. Wöckener A., Schuberth H.J. Freezing of maiden stallion semen-motility and morphological findings in sperm cells assessed by various staining methods including a monoclonal antibody with reactivity against an antigen in the acrosome ground substance. Reprod Dom Anim 1993, 28:265.
161. Cheng F.P., Gadella B.M., Voorhout W.F., et al. Progesterone-induced acrosome reaction in stallion spermatozoa is mediated by a plasma membrane progesterone receptor. Biol Reprod 1998, 59:733.
162. Christensen P., Whitfield C.H., Parkinson T.J. In vitro induction of acrosome reactions in stallion spermatozoa by heparin and A23187. Theriogenology 1996, 45:1201.
163. Varner D.D., Thompson J.A., Blanchard T., et al. Induction of the acrosome reaction in stallion spermatozoa-effects of incubation temperature, incubation time, and ionophore concentration. Theriogenology 2002, 58:303.
164. Gómez-Cuétera C., Squires E.L., Graham J.K. In vitro induction of the acrosome reaction in fresh and frozen-thawed equine spermatozoa. Anim Reprod Sci 2006, 94:165.
165. Varner D.D., Brinsko S.P., Blanchard T.L., et al. Subfertility in stallions associated with spermatozoal acrosome dysfunction in 2001. Proc AAEP 2001, 227.
166. Brinsko S.P., Love C.C., Bauer J.E., et al. Cholesterol-to-phospholipid ratio in whole sperm and seminal plasma from fertile stallions and stallions with unexplained subfertility. Anim Reprod Sci 2007, 99:65.
167. Brinsko S.P., Varner D.D., Love C.C., et al. Effect of feeding a DHA-enriched nutriceutical on the quality of fresh, cooled and frozen stallion semen. Theriogenology 2005, 63:1519.
168. Varner D.D., Bowen J.A., Johnson L. Effect of heparin on capacitation/acrosome reaction of equine sperm. Arch Andr 1993, 31:199.
169. Meyers S.A., Overstreet J.W., Liu I.K.M., et al. Capacitation in vitro of stallion spermatozoa: comparison of progesterone induced acrosome reactions in fertile and subfertile males. J Androl 1995, 16:47.
170. Meyers S.A., Liu I.K.M., Overstreet J.W., et al. Sperm-zona pellucida binding and zona-induced acrosome reactions in the horse: comparisons between fertile and subfertile stallions. Theriogenology 1996, 46:1277.
171. Rathi R., Nielen M., Cheng F.P., et al. Exposure of progesterone receptors on the plasma membranes of stallion spermatozoa as a parameter for prediction of fertility. J Reprod Fertil Suppl 2000, 56:87.
172. Fazeli A.R., Steenweg W., Bevers M.M., et al. Use of sperm binding to homologous hemizona pellucida to predict stallion fertility. Eq Vet J Suppl 1993, 15:57.
173. Fazeli A.R., Steenweg W., Bevers M., et al. Relation between stallion sperm binding to homologous hemizonae and fertility. Theriogenology 1995, 44:751.
174. Yanagimachi R., Yanagimachi H., Rogers B.J. The use of zona-free animal eggs as a test-system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod 1976, 15:471.
175. Landim-Alvarenga F.C., Alvarenga M.A., Seidel G.E. Penetration of zona-free hamster, bovine and equine oocytes by stallion and bull spermatozoa pretreated with equine follicular fluid, dilauroylphosphatidylcholine or calcium ionophore A23187. Theriogenology 2001, 56:937.
176. Thomas C.A., Garner D.L., DeJarnette J.M., et al. Effect of cryopreservation on bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 1998, 58:786.
177. Papaioannou K.Z., Murphy R.P., Monks R.S., et al. Assessment of viability and mitochondrial function of equine spermatozoa using double staining and flow cytometry. Theriogenology 1997, 48:299.
178. Turner R.M. Moving to the beat: a review of mammalian sperm motility regulation. Reprod Fertil Dev 2006, 18:25.
179. Gravance C.G., Garner D.L., Miller M.G., et al. Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function. Reprod Toxicol 2001, 15:5.
180. Evenson D.P., Darzynkiewicz Z., Melamed M.R. Relation of mammalian sperm chromatin heterogeneity to fertility. Science 1980, 210:1131.
181. Perreault S.D., Naish S.J., Zirkin B.R. The timing of hamster sperm nuclear decondensation and male pronucleus formation is related to sperm nuclear disulfide bond content. Biol Reprod 1987, 36:239.
182. Evenson D.P., Jost L.K., Varner D.D. Relationship between sperm nuclear protamine free-SH status and susceptibility to DNA denaturation. J Reprod Fertil Suppl 2000, 56:401.
183. Agarwal A., Said T.M. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod 2003, 9:331.
184. Evenson D.P., Jost L.K., Marshall D., et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reprod 1999, 14:1039.
185. Evenson D.P., Larson K.L., Jost L.K. Sperm chromatin structure assay: Its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 2002, 23:25.
186. Evenson D.P., Wixon R. Clinical aspects of sperm DNA fragmentation detection and male fertility. Theriogenology 2006, 65:979.
187. Ibrahim M.E., Pedersen H. Acridine orange fluorescence as male fertility test. Arch Androl 1988, 20:125.
188. Ball BA: Embryonic loss in mares. Incidence, possible causes, and diagnostic considerations. In van Camp SD, ed: Veterinary Clinics of North America Equine Practice. vol 263, 1988.
189. Evenson D.P., Sailer B.L., Jost L.K. Relationship between stallion sperm deoxyribonucleic acid (DNA) susceptibility to denaturation in situ and presence of DNA strand breaks: implications for fertility and embryo viability. Biol Reprod Mono 1995, 1:655.
190. Evenson D.P., Jost L.K., Baer R.K., et al. Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay. Reprod Toxicol 1991, 5:115.
191. Kenney R.M., Evenson D.P., Garcia M.C., et al. Relationship between sperm chromatin structure, motility, and morphology of ejaculated sperm and seasonal pregnancy rate. Biol Reprod Mono 1995, 1:647.
192. Love C.C., Kenney R.M. The relationship of increased susceptibility of sperm DNA to denaturation and fertility in the stallion. Theriogenology 1998, 50:955.
193. Larson K.L., DeJonge C.J., Barnes A.M., et al. Relationship of assisted reproductive technique (ART) outcomes with sperm chromatin integrity and maturity as measured by the sperm chromatin structure assay (SCSA). Human Reprod 2000, 15:1717.
194. Larson K.L., Brannian J., Hansen K., et al. Relationship between assisted reproductive techniques (ART) outcomes and DNA fragmentation (DFI) as measured by the Sperm Chromatin Structure Assay (SCSA). Am Soc Reprod Med, Seattle 2002, Oct 12-17.
195. Saleh R.A., Agarwal A., Nelson D.R., et al. Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study. Fertil Steril 2002, 78:313.
196. Morrell J.M., Johannisson A., Dalin A.M., et al. Sperm morphology and chromatin integrity in Swedish Warmblood stallions and their relationship to pregnancy rates. Acta Vet Scand 2008, 50:1.
197. Payne J.F., Raburn D.J., Couchman G.M., et al. Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques. Fertil Steril 2005, 84:356.
198. Bungum M., Humaidan P., Axmon A., et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Human Reprod 2007, 22:174.
199. Linfor J.J., Meyers S.A. Detection of DNA damage in response to cooling injury in equine spermatozoa using single-cell gel electrophoresis. J Androl 2002, 23:107.
200. Tejada R.I., Mitchell M.S., Norman A., et al. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril 1984, 42:87.
201. Garcia-Macias V., de Paz P., Martinez-Pastor F., et al. DNA fragmentation assessment by flow cytometry and Sperm-Bos-Halomax (bright-field microscopy and fluorescence microscopy) in bull sperm. Int J Androl 2007, 30:88.
202. Pesch S., Bergmann M., Bostedt H. Determination of some enzymes and macro- and microelements in stallion seminal plasma and their correlations to semen quality. Theriogenology 2006, 66:307.
203. Desvousges A.L., Dow C.A., Hayna J.T., et al. Heat shock induces apoptosis in equine spermatozoa. Anim Reprod Sci 2006, 94:125.
204. Brum A., Sabeur K., Ball B.A. Apoptotic-like changes in equine spermatozoa after separation by density-gradient centrifugation or after cryopreservation. Anim Reprod Sci 2006, 94:138.
205. Stradaioli G., Rubei M., Zamparini M., et al. Enzymatic evaluation of phospholipid hydroperoxide glutathione peroxidase (PHGPx) in equine spermatozoa. Anim Reprod Sci 2006, 94:29.
206. Neild D.M., Gadella B.M., Colenbrander B., et al. Lipid peroxidation in stallion spermatozoa. Theriogenology 2002, 58:295.
207. Neild D.M., Brouwers J.F.H., Colenbrander B., et al. Lipid peroxide formation in relation to membrane stability of fresh and frozen thawed stallion spermatozoa. Mol Reprod Dev 2005, 72:230.
208. Almeida J., Ball B.A. Effect of alpha-tocopherol and tocopherol succinate on lipid peroxidation in equine spermatozoa. Anim Reprod Sci 2005, 87:321.
209. Guthrie H.D., Welch G.R. Use of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with 4, 4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a, 4a-diaza-s-indacene-3-undecanoic acid. J Anim Sci 2007, 85:1402.
210. Aitken R.J. Sperm function tests and fertility. Int J Androl 2006, 29:69.
211. Ball B.A., Vo A.T., Baumber J. Generation of reactive oxygen species by equine spermatozoa. Am J Vet Res 2001, 62:508.
212. Guthrie H.D., Welch G.R. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J Anim Sci 2006, 84:2089.
213. Rodriguez-Martinez H., Iborra A., Martinez P., et al. Immunoelectron microscopic imaging of spermadhesin AWN epitopes on boar spermatozoa bound in vivo to the zona pellucida. Reprod Fertil Dev 1998, 10:491.
214. Gao A., Garbers D.L. Species diversity in the structure of zonadhesin, a sperm-specific membrane protein containing multiple cell adhesion molecule-like domains. J Biol Chem 1998, 273:3415.
215. Breazeale K.R., Brady H.A., Bi M., et al. Biochemical properties and localization of zonadhesin in equine spermatozoa. Theriogenology 2002, 58:359.
216. Bailey L.B., Brady H.B., Tardif S., et al. Zonadhesin expression and localization in stallion testes. Anim Reprod Sci 2006, 94:56.
217. Schambony A., Hess O., Gentzel M. Expression of CRISP proteins in the male equine genital tract. J Reprod Fertil Suppl 1998, 53:67.
218. Reineke A., Hess O., Schambony A., et al. Sperm-associated seminal plasma proteins-a novel approach for the evaluation of sperm fertilizing ability of stallions. Pferdeheilkunde 1999, 6:531.
219. Jude R., Giese A., Piumi F., et al. Molecular characterization of the CRISP 1 gene-a candidate gene for stallion fertility. Theriogenology 2002, 58:417.
220. Turner R.M., Sertich P.L. Use of alkaline phosphatase as a diagnostic tool in stallions with azoospermia and oligospermia. Anim Reprod Sci 2001, 68:315.
221. Turner R.M., McDonnell S.M. Alkaline phosphatase in stallion semen characterization and clinical applications. Theriogenology 2003, 60:1.
222. Ball B.A., Baumber J., Vo A., et al. Effect of oxidative stress on equine spermatozoa. Anim Reprod Sci 2001, 68:364.
223. Burnaugh L., Sabeur K., Ball B.A. Generation of superoxide anion by equine spermatozoa as detected by dihydroethidium. Theriogenology 2007, 67:580.
224. Stradaioli G., Gennevieve A., Magistrini M. Evaluation of lipid peroxidation by thiobarbituric acid test in equine spermatozoa. Anim Reprod Sci 2001, 68:320.
225. Morte M.E., Rodrigues A.M., Soares D., et al. The quantification of lipid and protein oxidation in stallion spermatozoa and seminal plasma: Seasonal distinctions and correlations with DNA strand breaks, classical seminal parameters and stallion fertility. Anim Reprod Sci 2008, 106:36.
226. Baumber J., Ball B.A., Linfor J.J., et al. Reactive oxygen species and cryopreservation promote deoxyribonucleic acid (DNA) damage in equine sperm. Theriogenology 2003, 58:301.
227. Sullivan R. Male fertility markers, myth or reality. Anim Reprod Sci 2004, 82:341.
228. Leeb T., Sieme H., Töpfer-Petersen E. Genetic markers for stallion fertility-lessons from humans and mice. Anim Reprod Sci 2005, 89:21.
229. Chenoweth P.J. Genetic sperm defects. Theriogenology 2005, 64:457.
230. Chowdhary B.P., Taudsepp T., Kata S.R., et al. The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes. Genome Res 2003, 13:742.
231. Tozaki T., Hirota K.I., Hasegawa T., et al. Whole-genome linkage disequilibrium screening for complex traits in horses. Mol Genet Genomics 2007, 277:663.
232. Chowdhary B.P., Paria N., Raudsepp T. Potential applications of equine genomics in dissecting diseases and fertility. Anim Reprod Sci 2008, 107:208.
233. He Z., Chan W.Y.C., Dym M. Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility. Reproduction 2006, 132:11.
234. Hamann H., Jude R., Sieme H., et al. A polymorphism within the equine CRISP3 gene is associated with stallion fertility in Hanoverian Warmblood horses. Anim Genet 2007, 38:259.
235. Thiessen H. Fluorescence microscopic analysis of the sperm chromatin structure in the stallion. Thesis, Hannover 2006.