Comparison of FACSCount AF system, Improved Neubauer hemocytometer, Corning 254 photometer, SpermVision, UltiMate and NucleoCounter SP-100 for determination of sperm concentration of boar semen

Theriogenology

Volumn 66, Issue 9, 2006, Pages 2188-2194

  Hansen, C ^a   Vermeiden, T ^b   Vermeiden, J P W ^c   Simmet, C ^d   Day, B C ^e   Feitsma, H ^f  

^a Danish Meat Association   (Denmark)

^b LEJA Products BV   (Netherlands)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^d MINITÜB Abfüll und Labortechnik GmbH   (Germany)

^e Minitube of America   (United States)

^f Dutch Association of Co operative Pig AI Centres   (Netherlands)

Author keywords

Boar; CASA; Counting chamber; Reproduction; Semen; Sperm concentration

Indexed keywords

FLUORESCENT DYE;

ANIMAL; ANIMAL DISEASE; ARTICLE; COMPARATIVE STUDY; CYTOLOGY; FLOW CYTOMETRY; INSTRUMENTATION; MALE; METHODOLOGY; REGRESSION ANALYSIS; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY; SPERM; SPERMATOZOON COUNT; STANDARD; SWINE;

ANIMALS; FLOW CYTOMETRY; FLUORESCENT DYES; MALE; REGRESSION ANALYSIS; REPRODUCIBILITY OF RESULTS; SEMEN; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY; SPERM COUNT; SWINE;

SUS SCROFA;

EID: 33846007996     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2006.05.020     Document Type: Article

References (11)

1. Hedeboe AM. 2 kontra 1 mia. sædceller i sæddoserne. The National Committee for Pig Production in Denmark. Report no. 717; 2005.
2. World Health Organization. WHO laboratory amnual for the examination of human semen and sperm-cervical mucos interaction. 4th ed. Cambridge University Press; 1999.
3. Seaman E.K., Goluboff E., BarChama N., and Fisch H. Accuracy of semen counting chambers as determined by the use of latex beads. Fertil Steril 66 (1996) 662-665
4. Mahmoud A.M., Depoorter B., Piens N., and Comhaire F.H. The performance of 10 different methods for the estimation of sperm concentration. Fertil Steril 68 (1997) 340-345
5. Ginsburg K.A., and Armant D.R. The influence of chamber characteristics on the reliability of sperm concentration and movement measurements obtained by manual and videomicrographic analysis. Fertil Steril 53 (1990) 882-887
6. Hansen C., Christensen P., Stryhn H., et al. Validation of the FACSCount AF system for determination of sperm concentration in boar semen. Reprod Domest Anim 37 (2002) 330-334
7. Douglas-Hamilton D.H., Smith N.G., Kuster C.E., Vermeiden J.P., and Althouse G.C. Capillary-loaded particle fluid dynamics: effect on estimation of sperm concentration. J Androl 26 (2005) 115-122
8. Shiran E., Stoller J., Blumenfeld Z., Feigin P.D., and Makler A. Evaluating the accuracy of different sperm counting chambers by performing strict counts of photographed beads. J Assist Reprod Genet 12 (1995) 434-442
9. Christensen P., Hansen C., Liboriussen T., and Lehn-Jensen H. Implementation of flow cytometry for quality control in four Danish bull studs. Anim Reprod Sci 85 (2005) 201-208
10. Hansen C, Hedeboe AM. Determining concentration of raw semen: test of the NucleoCounter SP-100, MiniTüb SDM5 and Corning 254. The National Committee for Pig Production in Denmark. Report no. 613; 2003.
11. Douglas-Hamilton D.H., Smith N.G., Kuster C.E., Vermeiden J.P., and Althouse G.C. Particle distribution in low-volume capillary-loaded chambers. J Androl 26 (2005) 107-114