Evolution of testing algorithms at a university hospital for detection of Clostridium difficile infections

Journal of Clinical Microbiology

Volumn 50, Issue 9, 2012, Pages 3073-3076

  Culbreath, Karissa ^a,c   Ager, Edward ^a,d   Nemeyer, Ronald J ^a   Kerr, Alan ^a   Gilligan, Peter H ^a,b  

^a UNIVERSITY OF NORTH CAROLINA HOSPITALS   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c TRICORE REFERENCE LABORATORIES   (United States)

^d LANDSTUHL REGIONAL MEDICAL CENTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN; CLOSTRIDIUM DIFFICILEN TOXIN A; CLOSTRIDIUM DIFFICILEN TOXIN B; GLUTAMATE DEHYDROGENASE; UNCLASSIFIED DRUG;

ALGORITHM; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CLOSTRIDIUM DIFFICILE INFECTION; CLOSTRIDIUM DIFFICILE QUICK CHECK COMPLETE; COST EFFECTIVENESS ANALYSIS; FECES ANALYSIS; GDH IMMUNOCHROMATOGRAPHIC CARTRIDGE ASSAY; GENEXPERT CLOSTRIDIUM DIFFICILE; IMMUNOASSAY; NONHUMAN; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; RELIABILITY; SCREENING TEST;

ALGORITHMS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; ENTEROTOXINS; GLUTAMATE DEHYDROGENASE; HOSPITALS, UNIVERSITY; HUMANS; IMMUNOASSAY; POLYMERASE CHAIN REACTION;

EID: 84865174667     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00992-12     Document Type: Article

References (26)

1. Bauer MP, Kuijper EJ, van Dissel JT. 2009. European Society of Clinical Microbiology and Infectious Diseases (ESCMID): treatment guidance document for Clostridium difficile infection (CDI). Clin. Microbiol. Infect. 15:1067-1079.
2. Cohen SH, et al. 2010. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect. Control Hosp. Epidemiol. 31:431-455.
3. Doing KM, et al. 2010. Reevaluation of the Premier Clostridium difficile toxin A and B immunoassay with comparison to glutamate dehydrogenase common antigen testing evaluating Bartels cytotoxin and Prodesse ProGastro Cd polymerase chain reaction as confirmatory procedures. Diagn. Microbiol. Infect. Dis. 66:129-134.
4. Dubberke ER, et al. 2011. Impact of clinical symptoms on the interpretation of diagnostic assays for Clostridium difficile. J. Clin. Microbiol. 49: 2887-2893.
5. Eastwood K, Else P, Charlett A, Wilcox M. 2009. Comparison of nine commercially available Clostridium difficile toxin detection assays, a realtime PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 47:3211-3217.
6. Fenner L, Widmer AF, Goy G, Rudin S, Frei R. 2008. Rapid and reliable diagnostic algorithm for detection of Clostridium difficile. J. Clin. Microbiol. 46:328-330.
7. Fong KS, et al. 2011. Impact of PCR testing for Clostridium difficile on incident rates and potential of public reporting: is the playing field level? Infect. Control Hosp. Epidemiol. 32:932-933.
8. Gilligan PH. 2008. Is a two-step glutamate dehydrogenase antigen-cytotoxicity neutralization assay algorithm superior to the Premier toxin A and B enzyme immunoassay for laboratory detection of Clostridium difficile? J. Clin. Microbiol. 46:1523-1525. (Pubitemid 351563608)
9. Goldenberg SD, Cliff PR, French GL. 2010. Laboratory diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 48:3048-3049.
10. Karre T, Sloan L, Patel R, Mandrekar J, Rosenblatt J. 2011. Comparison of two commercial molecular assays to a laboratory-developed molecular assay for diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 49:725-727.
11. Kvach EJ, Ferguson D, Riska PF, Landry ML. 2010. Comparison of BD GeneOhm Cdiff real-time PCR assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection. J. Clin. Microbiol. 48:109-114.
12. Lalande V, et al. 2011. Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections. J. Clin. Microbiol. 49:2714-2716.
13. Larson AM, Fung AM, Fang FC. 2010. Evaluation of tcdB real-time PCR in a three-step diagnostic algorithm for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 48:124-130.
14. Luna RA, et al. 2011. Rapid stool-based diagnosis of Clostridium difficile infection by real-time PCR in a children's hospital. J. Clin. Microbiol. 49:851-857.
15. Luo RF, Banaei N. 2010. Is repeat PCR needed for diagnosis of Clostridium difficile infection? J. Clin. Microbiol. 48:3738-3741.
16. Novak-Weekley SM, et al. 2010. Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms. J. Clin. Microbiol. 48: 889-893.
17. Peterson LR, et al. 2011. Laboratory testing for Clostridium difficile infection: light at the end of the tunnel. Am. J. Clin. Pathol. 136:372-380.
18. Quinn CD, et al. 2010. C. Diff Quik Chek Complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens. J. Clin. Microbiol. 48:603-605.
19. Sharp SE, et al. 2010. Evaluation of the C.Diff Quik Chek Complete assay, a new glutamate dehydrogenase and A/B toxin combination lateral flow assay for use in rapid, simple diagnosis of Clostridium difficile disease. J. Clin. Microbiol. 48:2082-2086.
20. Shetty N, Wren MW, Coen PG. 2011. The role of glutamate dehydrogenase for the detection of Clostridium difficile in faecal samples: a meta-analysis. J. Hosp. Infect. 77:1-6.
21. Surawicz CM, et al. 2000. The search for a better treatment for recurrent Clostridium difficile disease: use of high-dose vancomycin combined with Saccharomyces boulardii. Clin. Infect. Dis. 31:1012-1017. (Pubitemid 32299362)
22. Swindells J, Brenwald N, Reading N, Oppenheim B. 2010. Evaluation of diagnostic tests for Clostridium difficile infection. J. Clin. Microbiol. 48: 606-608.
23. Tenover FC, et al. 2010. Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J. Clin. Microbiol. 48:3719-3724.
24. Ticehurst JR, et al. 2006. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 44:1145-1149.
25. Wenisch C, Parschalk B, Hansenhundl M, Hirschl AM, Graninger W. 1996. Comparison of vancomycin, teicoplanin, metronidazole, and fusidic acid for the treatment of Clostridium difficile-associated diarrhea. Clin. Infect. Dis. 22:813-818. (Pubitemid 26255600)
26. Wilcox MH, Planche T, Fang FC, Gilligan P. 2010. What is the current role of algorithmic approaches for diagnosis of Clostridium difficile infection? J. Clin. Microbiol. 48:4347-4353.