Comparison of two commercial molecular assays to a laboratory-developed molecular assay for diagnosis of Clostridium difficile infection

Journal of Clinical Microbiology

Volumn 49, Issue 2, 2011, Pages 725-727

  Karre, Tess ^a   Sloan, Lynne ^a   Patel, Robin ^a   Mandrekar, Jayawant ^a   Rosenblatt, Jon ^a  

^a MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CLOSTRIDIUM DIFFICILE INFECTION; COMPARATIVE STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FECES ANALYSIS; HUMAN; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; FECES; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY;

CLOSTRIDIUM DIFFICILE;

EID: 79951481951     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01028-10     Document Type: Article

References (14)

1. Alcala, L., et al. 2008. Comparison of three commercial methods for rapid detection of Clostridium difficile toxins A and B from fecal specimens. J. Clin. Microbiol. 46:3833-3835.
2. Aldeen, W. E., et al. 2000. Comparison of the TOX A/B test to a cell culture cytotoxicity assay for the detection of Clostridium difficile in stools. Diagn. Microbiol. Infect. Dis. 36:211-213. (Pubitemid 30179641)
3. Arzese, A., G. Trani, L. Riul, and G. A. Botta. 1995. Rapid polymerase chain reaction method for specific detection of toxigenic Clostridium difficile. Eur. J. Clin. Microbiol. Infect. Dis. 14:716-719.
4. Barbut, F., M. Braun, B. Burghoffer, V. Lalande, and C. Eckert. 2009. Rapid detection of toxigenic strains of Clostridium difficile in diarrheal stools by real-time PCR. J. Clin. Microbiol. 47:1276-1277.
5. Bartlett, J. G. 2002. Clinical practice: antibiotic-associated diarrhea. N. Engl. J. Med. 346:334-339.
6. Bartlett, J. G. 1994. Clostridium difficile: history of its role as an enteric pathogen and the current state of knowledge about the organism. Clin. Infect. Dis. 18(Suppl. 4):S265-S272.
7. Belanger, S. D., M. Boissinot, N. Clairoux, F. J. Picard, and M. G. Bergeron. 2003. Rapid detection of Clostridium difficile in feces by real-time PCR. J. Clin. Microbiol. 41:730-734.
8. Eastwood, K., P. Else, A. Charlett, and M. Wilcox. 2009. Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 47:3211-3217.
9. Musher, D. M., et al. 2007. Detection of Clostridium difficile toxin: comparison of enzyme immunoassay results with results obtained by cytotoxicity assay. J. Clin. Microbiol. 45:2737-2739.
10. Peterson, L. R., et al. 2007. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin. Infect. Dis. 45:1152-1160. (Pubitemid 47580313)
11. Sloan, L. M., B. J. Duresko, D. R. Gustafson, and J. E. Rosenblatt. 2008. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 46:1996-2001.
12. Ticehurst, J. R., et al. 2006. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 44:1145-1149.
13. Turgeon, D. K., et al. 2003. Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay. J. Clin. Microbiol. 41:667-670.
14. van den Berg, R. J., et al. 2007. Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study. J. Med. Microbiol. 56:36-42.