Ultrafast studies of the photophysics of Cis and trans states of the green fluorescent protein chromophore

Journal of Physical Chemistry Letters

Volumn 3, Issue 16, 2012, Pages 2298-2302

  Addison, Kiri ^a   Conyard, Jamie ^a   Dixon, Tara ^a   Bulman Page, Philip C ^a   Solntsev, Kyril M ^b   Meech, Stephen R ^a  

^a UNIVERSITY OF EAST ANGLIA   (United Kingdom)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CIS-TRANS PHOTOISOMERIZATION; DARK STATE; DIFFERENTIAL INTERACTION; FLUORESCENCE MEASUREMENTS; FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; KEY PROCESS; MEAN LIFETIME; PHOTOACTIVATABLE; PHOTOACTIVE PROTEINS; PHOTOPHYSICS; PHOTOSWITCHING; TRANS STATE; ULTRA-FAST;

FLUORESCENCE; ISOMERS; PROTEINS;

CHROMOPHORES;

EID: 84865084357     PISSN: None     EISSN: 19487185     Source Type: Journal    
DOI: 10.1021/jz3008408     Document Type: Article

References (46)

1. Shimomura, O.; Johnson, F. H.; Saiga, Y. Extraction, Purification and Properties of Aequorin, a Bioluminescent Protein from the Luminous Hydromedusan, Aequorea J. Cell. Comp. Physiol. 1962, 59, 223-239
2. Tsien, R. Y. The Green Fluorescent Protein Annu. Rev. Biochem. 1998, 67, 509-544
3. Chudakov, D. M.; Matz, M. V.; Lukyanov, S.; Lukyanov, K. A. Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues Physiol. Rev. 2010, 90, 1103-63
4. Shaner, N. C.; Campbell, R. E.; Steinbach, P. A.; Giepmans, B. N.; Palmer, A. E.; Tsien, R. Y. Improved Monomeric Red, Orange and Yellow Fluorescent Proteins Derived from Discosoma sp. Red Fluorescent Protein Nat. Biotechnol. 2004, 22, 1567-72
5. Wang, L.; Jackson, W. C.; Steinbach, P. A.; Tsien, R. Y. Evolution of New Nonantibody Proteins via Iterative Somatic Hypermutation Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 16745-16749
6. Tsien, R. Y. Constructing and Exploiting the Fluorescent Protein Paintbox (Nobel Lecture) Angew. Chem., Int. Ed. 2009, 48, 5612-26
7. Lukyanov, K. A.; Chudakov, D. M.; Lukyanov, S.; Verkhusha, V. V. Photoactivatable Fluorescent Proteins Nat. Rev. Mol. Cell Biol. 2005, 6, 885-890
8. Hofmann, M.; Eggeling, C.; Jakobs, S.; Hell, S. W. Breaking the Diffraction Barrier in Fluorescence Microscopy at Low Light Intensities by Using Reversibly Photoswitchable Proteins Proc. Natl. Acad. Sci. U. S. A. 2005, 102, 17565-9
9. Schwentker, A.; Bock, M., H.; Hofmann, M.; Jakobs, S.; Bewersdorf, J.; Eggeling, C.; Hell, S. W. Wide-Field Subdiffraction RESOLFT Microscopy Using Fluorescent Protein Photoswitching Microsc. Res. Tech. 2007, 70, 269-280
10. Adam, V. Data Storage Based on Photochromic and Photoconvertible Fluorescent Proteins J. Biotechnol. 2010, 149, 289-298
11. Ando, R.; Hama, H.; Yamamoto-Hino, M.; Mizuno, H.; Miyawaki, A. An Optical Marker Based on The UV-Induced Green-to-Red Photoconversion of a Fluorescent Protein Proc. Natl. Acad. Sci. U. S. A. 2002, 99, 12651-6
12. Mizuno, H.; Mal, T. K.; Tong, K. I.; Ando, R.; Furuta, T.; Ikura, M.; Miyawaki, A. Photo-Induced Peptide Cleavage in the Green-to-Red Conversion of a Fluorescent Protein Mol. Cell 2003, 12, 1051-1058
13. Habuchi, S.; Ando, R.; Dedecker, P.; Verheijen, W.; Mizuno, H.; Miyawaki, A.; Hofkens, J. Reversible Single-Molecule Photoswitching in the GFP-Like Fluorescent Protein Dronpa Proc. Natl. Acad. Sci. U. S. A. 2005, 102, 9511-6
14. Koseki, J.; Kita, Y.; Nagashima, U.; Tachikawa, M. Theoretical Study of the Reversible Photoconversion Mechanism in Dronpa Procedia Comput. Sci. 2011, 4, 251-260
15. Andresen, M.; Stiel, A. C.; Trowitzsch, S.; Weber, G.; Eggeling, C.; Wahl, M. C.; Hell, S. W.; Jakobs, S. Structural Basis for Reversible Photoswitching in Dronpa Proc. Natl. Acad. Sci. U. S. A. 2007, 104, 13005-13009
16. Li, X.; Chung, L. W.; Mizuno, H.; Miyawaki, A.; Morokuma, K. Primary Events of Photodynamics in Reversible Photoswitching Fluorescent Protein Dronpa J. Phys. Chem. Lett. 2010, 1, 3328-3333
17. Mizuno, H.; Kumar Mal, T.; Walchli, M.; Kikuchi, A.; Fukano, T.; Ando, R.; Jeyakanthan, J.; Taka, J.; Shiro, Y.; Ikura, M.; Miyawaki, A. Light-Dependent Regulation of Structural Flexibility in a Photochromic Fluorescent Protein Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 9227-9232
18. Nam, K.-H. Structural Characterization of the Photoswitchable Fluorescent Protein Dronpa-C62S Biochem. Biophys. Res. Commun. 2007, 354, 962-967
19. Wilmann, P. G.; Turcic, K.; Battad, J. M.; Wilce, M. C. J.; Devenish, R. J.; Prescott, M.; Rossjohn, J. The 1.7 Å Crystal Structure of Dronpa: A Photoswitchable Green Fluorescent Protein J. Mol. Biol. 2006, 364, 213-224
20. Schäfer, L. V.; Groenhof, G.; Boggio-Pasqua, M.; Robb, M. A.; Grubmüller, H. Chromophore Protonation State Controls Photoswitching of the Fluoroprotein asFP595 PLoS Comput Biol. 2008, 4, e1000034
21. Habuchi, S.; Dedecker, P.; Hotta, J.; Flors, C.; Ando, R.; Mizuno, H.; Miyawaki, A.; Hofkens, J. Photo-Induced Protonation/Deprotonation in the GFP-Like Fluorescent Protein Dronpa: Mechanism Responsible for the Reversible Photoswitching Photochem. Photobiol. Sci. 2006, 5, 567-576
22. Regis Faro, A.; Carpentier, P.; Jonasson, G.; Pompidor, G.; Arcizet, D.; Demachy, I.; Bourgeois, D. Low-Temperature Chromophore Isomerization Reveals the Photoswitching Mechanism of the Fluorescent Protein Padron J. Am. Chem. Soc. 2011, 133, 16362-16365
23. Cody, C. W.; Prasher, D. C.; Westler, W. M.; Prendergast, F. G.; Ward, W. W. Chemical Structure of the Hexapeptide Chromophore of the Aequorea Green-Fluorescent Protein Biochemistry 1993, 32, 1212-1218
24. Reid, B. G.; Flynn, G. C. Chromophore Formation in Green Fluorescent Protein Biochemistry 1997, 36, 6786-6791
25. Tolbert, L. M.; Baldridge, A.; Kowalik, J.; Solntsev, K. M. Collapse and Recovery of Green Fluorescent Protein Chromophore Emission through Topological Effects Acc. Chem. Res. 2012, 45, 171-181
26. Bravaya, K. B.; Grigorenko, B. L.; Nemukhin, A. V.; Krylov, A. I. Quantum Chemistry Behind Bioimaging: Insights from Ab Initio Studies of Fluorescent Proteins and Their Chromophores Acc. Chem. Res. 2012, 45, 265-275
27. Meech, S. R. Excited State Reactions in Fluorescent Proteins Chem. Soc. Rev. 2009, 38, 2922-2934
28. Tonge, P. J.; Meech, S. R. Excited State Dynamics in the Green Fluorescent Protein J. Photochem. Photobiol., A 2009, 205, 1-11
29. Voliani, V.; Bizzarri, R.; Nifosì, R.; Abbruzzetti, S.; Grandi, E.; Viappiani, C.; Beltram, F. Cis-Trans Photoisomerization of Fluorescent-Protein Chromophores J. Phys. Chem. B. 2008, 112, 10714-10722
30. Yang, J.-S.; Huang, G.-J.; Liu, Y.-H.; Peng, S.-M. Photoisomerization of the Green Fluorescence Protein Chromophore and the meta- and para-Amino Analogues Chem. Commun. 2008, 1344-1346
31. Dong, J.; Abulwerdi, F.; Baldridge, A.; Kowalik, J.; Solntsev, K. M.; Tolbert, L. M. Isomerization in Fluorescent Protein Chromophores Involves Addition/Elimination J. Am. Chem. Soc. 2008, 130, 14096-14098
32. Dong, J.; Solntsev, K. M.; Poizat, O.; Tolbert, L. M. The Meta-Green Fluorescent Protein Chromophore J. Am. Chem. Soc. 2007, 129, 10084-10085
33. He, X.; Bell, A. F.; Tonge, P. J. Ground State Isomerization of a Model Green Fluorescent Protein Chromophore FEBS Lett. 2003, 549, 35-38
34. Brejc, K.; Sixma, T. K.; Kitts, P. A.; Kain, S. R.; Tsien, R. Y.; Ormo, M.; Remington, S. J. Structural Basis for Dual Excitation and Photoisomerization of the Aequorea victoria Green Fluorescent Protein Proc. Natl. Acad. Sci. U. S. A. 1997, 94, 2306-11
35. Stoner-Ma, D.; Jaye, A. A.; Matousek, P.; Towrie, M.; Meech, S. R.; Tonge, P. J. Observation of Excited-State Proton Transfer in Green Fluorescent Protein Using Ultrafast Vibrational Spectroscopy J. Am. Chem. Soc. 2005, 127, 2864-2865
36. Chattoraj, M.; King, B. A.; Bublitz, G. U.; Boxer, S. G. Ultra-Fast Excited State Dynamics in Green Fluorescent Protein: Multiple States and Proton Transfer Proc. Natl. Acad. Sci. U. S. A. 1996, 93, 8362-8367
37. Conyard, J.; Kondo, M.; Heisler, I. A.; Jones, G.; Baldridge, A.; Tolbert, L. M.; Solntsev, K. M.; Meech, S. R. Chemically Modulating the Photophysics of the GFP Chromophore J. Phys. Chem. B. 2011, 115, 1571-1577
38. Mandal, D.; Tahara, T.; Meech, S. R. Excited-State Dynamics in the Green Fluorescent Protein Chromophore J. Phys. Chem. B. 2003, 108, 1102-1108
39. Ben-Amotz, D.; Harris, C. B. Torsional Dynamics of Molecules on Barrierless Potentials in Liquids. I. Temperature and Wavelength Dependent Picosecond Studies of Triphenyl-Methane Dyes J. Chem. Phys. 1987, 86, 4856-4870
40. Bagchi, B.; Fleming, G. R.; Oxtoby, D. W. Theory of Electronic Relaxation in Solution in the Absence of an Activation Barrier J. Chem. Phys. 1983, 78, 7375-7385
41. Bagchi, B.; Fleming, G. R. Dynamics of Activationless Reactions in Solution J. Phys. Chem. 1990, 94, 9-20
42. Litvinenko, K. L.; Webber, N. M.; Meech, S. R. Internal Conversion in the Chromophore of the Green Fluorescent Protein: Temperature Dependence and Isoviscosity Analysis J. Phys. Chem. A. 2003, 107, 2616-2623
43. Gepshtein, R.; Huppert, D.; Agmon, N. Deactivation Mechanism of the Green Fluorescent Chromophore J. Phys. Chem. B. 2006, 110, 4434-4442
44. Olsen, S.; Smith, S. C. Bond Selection in the Photoisomerization Reaction of Anionic Green Fluorescent Protein and Kindling Fluorescent Protein Chromophore Models J. Am. Chem. Soc. 2008, 130, 8677-8689
45. Kao, Y.-T.; Zhu, X.; Min, W. Protein-Flexibility Mediated Coupling between Photoswitching Kinetics and Surrounding Viscosity of a Photochromic Fluorescent Protein Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 3220-3225
46. Heisler, I. A.; Kondo, M.; Meech, S. R. Reactive Dynamics in Confined Liquids: Ultrafast Torsional Dynamics of Auramine O in Nanoconfined Water in Aerosol OT Reverse Micelles J. Phys. Chem. B. 2009, 113, 1623-1631