FRAP analysis: Accounting for bleaching during image capture

PLoS ONE

Volumn 7, Issue 8, 2012, Pages

  Wu, Jun ^a   Shekhar, Nandini ^a   Lele, Pushkar P ^b   Lele, Tanmay P ^a  

^a University of Florida   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLEACHING; CONFOCAL MICROSCOPY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; IMAGE ANALYSIS; IMAGE PROCESSING; MATHEMATICAL ANALYSIS; MATHEMATICAL MODEL; PLASMID; PROMOTER REGION; RECEPTOR BINDING;

ANIMALS; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; FOCAL ADHESIONS; IMMOBILIZED PROTEINS; MICE; MODELS, THEORETICAL; MOLECULAR IMAGING; NIH 3T3 CELLS; PHOTOBLEACHING;

EID: 84864993491     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0042854     Document Type: Article

References (25)

1. Mueller F, Mazza D, Stasevich TJ, McNally JG, ((2010)) FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know? Curr Opin Cell Biol 22:: 403--411.
2. Mueller F, Karpova TS, Mazza D, McNally JG, ((2012)) Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching. Methods Mol Biol 833:: 153--176.
3. Phair RD, Gorski SA, Misteli T, ((2004)) Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy. Methods Enzymol 375:: 393--414.
4. Phair RD, Misteli T, ((2001)) Kinetic modelling approaches to in vivo imaging. Nat Rev Mol Cell Biol 2:: 898--907.
5. Kaufman EN, Jain RK, ((1991)) Measurement of mass transport and reaction parameters in bulk solution using photobleaching. Reaction limited binding regime. Biophys J 60:: 596--610.
6. Kaufman EN, Jain RK, ((1990)) Quantification of transport and binding parameters using fluorescence recovery after photobleaching. Potential for in vivo applications. Biophys J 58:: 873--885.
7. Stenoien DL, Patel K, Mancini MG, Dutertre M, Smith CL, et al. ((2001)) FRAP reveals that mobility of oestrogen receptor-alpha is ligand- and proteasome-dependent. Nat Cell Biol 3:: 15--23.
8. Wagner S, Chiosea S, Ivshina M, Nickerson JA, ((2004)) In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160. J Cell Biol 164:: 843--850.
9. Deschout H, Hagman J, Fransson S, Jonasson J, Rudemo M, et al. ((2010)) Straightforward FRAP for quantitative diffusion measurements with a laser scanning microscope. Opt Express 18:: 22886--22905.
10. Smisdom N, Braeckmans K, Deschout H, vandeVen M, Rigo JM, et al. ((2011)) Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk. J Biomed Opt 16:: 046021.
11. Stasevich TJ, Mueller F, Michelman-Ribeiro A, Rosales T, Knutson JR, et al. ((2010)) Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates. Biophys J 99:: 3093--3101.
12. Sprague BL, Pego RL, Stavreva DA, McNally JG, ((2004)) Analysis of binding reactions by fluorescence recovery after photobleaching. Biophys J 86:: 3473--3495.
13. Sprague BL, McNally JG, ((2005)) FRAP analysis of binding: proper and fitting. Trends Cell Biol 15:: 84--91.
14. Braeckmans K, Remaut K, Vandenbroucke RE, Lucas B, De Smedt SC, et al. ((2007)) Line FRAP with the confocal laser scanning microscope for diffusion measurements in small regions of 3-D samples. Biophys J 92:: 2172--2183.
15. Kang M, Day CA, DiBenedetto E, Kenworthy AK, ((2010)) A quantitative approach to analyze binding diffusion kinetics by confocal FRAP. Biophys J 99:: 2737--2747.
16. Kang M, Day CA, Drake K, Kenworthy AK, DiBenedetto E, ((2009)) A generalization of theory for two-dimensional fluorescence recovery after photobleaching applicable to confocal laser scanning microscopes. Biophys J 97:: 1501--1511.
17. Kang M, Kenworthy AK, ((2008)) A closed-form analytic expression for FRAP formula for the binding diffusion model. Biophys J 95:: L13--15.
18. van Royen ME, Farla P, Mattern KA, Geverts B, Trapman J, et al. ((2009)) Fluorescence recovery after photobleaching (FRAP) to study nuclear protein dynamics in living cells. Methods Mol Biol 464:: 363--385.
19. Benson DM, Bryan J, Plant AL, Gotto AM Jr, Smith LC, ((1985)) Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells. J Cell Biol 100:: 1309--1323.
20. Dragestein KA, van Cappellen WA, van Haren J, Tsibidis GD, Akhmanova A, et al. ((2008)) Dynamic behavior of GFP-CLIP-170 reveals fast protein turnover on microtubule plus ends. J Cell Biol 180:: 729--737.
21. Lele TP, Pendse J, Kumar S, Salanga M, Karavitis J, et al. ((2006)) Mechanical forces alter zyxin unbinding kinetics within focal adhesions of living cells. J Cell Physiol 207:: 187--194.
22. Lele TP, Ingber DE, ((2006)) A mathematical model to determine molecular kinetic rate constants under non-steady state conditions using fluorescence recovery after photobleaching (FRAP). Biophys Chem 120:: 32--35.
23. Lele TP, Thodeti CK, Pendse J, Ingber DE, ((2008)) Investigating complexity of protein-protein interactions in focal adhesions. Biochem Biophys Res Commun 369:: 929--934.
24. Sharp ZD, Mancini MG, Hinojos CA, Dai F, Berno V, et al. ((2006)) Estrogen-receptor-alpha exchange and chromatin dynamics are ligand- and domain-dependent. J Cell Sci 119:: 4101--4116.
25. Bulinski JC, Odde DJ, Howell BJ, Salmon TD, Waterman-Storer CM, ((2001)) Rapid dynamics of the microtubule binding of ensconsin in vivo. J Cell Sci 114:: 3885--3897.