Direct detection of nasal Staphylococcus aureus carriage via helicase-dependent isothermal amplification and chip hybridization

BMC Research Notes

Volumn 5, Issue , 2012, Pages

  Frech, Georges C ^a   Munns, Denton ^a   Jenison, Robert D ^a   Hicke, Brian J ^a  

^a Great Basin Corporation   (United States)

Author keywords

Helicase dependent amplification; Molecular diagnostic; Nasal carriage; Staphylococcus aureus

Indexed keywords

BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS AUREUS;

BACTERIAL DNA; PRIMER DNA;

ARTICLE; BACTERIAL GENE; GENETICS; HETEROZYGOTE; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; NOSE CAVITY; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INFECTION;

BASE SEQUENCE; CARRIER STATE; DNA PRIMERS; DNA, BACTERIAL; GENES, BACTERIAL; HUMANS; NASAL CAVITY; NUCLEIC ACID HYBRIDIZATION; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 84864823112     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-5-430     Document Type: Article

References (40)

1. The bacteria fight back. Taubes G, Science 2008 321 356 361 10.1126/science.321.5887.356 18635788 (Pubitemid 352029937)
2. The burden of Staphylococcus aureus infections on hospitals in the United States: an analysis of the 2000 and 2001 Nationwide Inpatient Sample Database. Noskin GA, Rubin RJ, Schentag JJ, Kluytmans J, Hedblom EC, Smulders M, Lapetina E, Gemmen E, Arch Intern Med 2005 165 1756 1761 10.1001/archinte.165.15.1756 16087824 (Pubitemid 41138964)
3. Prevalence of Staphylococcus aureus nasal colonization in the United States, 2001-2002. Kuehnert MJ, Kruszon-Moran D, Hill HA, McQuillan G, McAllister SK, Fosheim G, McDougal LK, Chaitram J, Jensen B, Fridkin SK, Killgore G, Tenover FC, J Infect Dis 2006 193 172 179 10.1086/499632 16362880 (Pubitemid 43113478)
4. The role of nasal carriage in Staphylococcus aureus infections. Wertheim HF, Melles DC, Vos MC, van Leeuwen W, van Belkum A, Verbrugh HA, Nouwen JL, Lancet Infect Dis 2005 5 751 762 10.1016/S1473-3099(05)70295-4 16310147 (Pubitemid 41688980)
5. Quantitative studies of nasal carriers of staphylococci among hospitalized patients. White A, J Clin Invest 1961 40 23 30 10.1172/JCI104233 13784803
6. Quantitative analysis and molecular fingerprinting of methicillin-resistant Staphylococcus aureus nasal colonization in different patient populations: a prospective, multicenter study. Mermel LA, Eells SJ, Acharya MK, Cartony JM, Dacus D, Fadem S, Gay EA, Gordon S, Lonks JR, Perl TM, McDougal LK, McGowan JE, Maxey G, Morse D, Tenover FC, Infect Control Hosp Epidemiol 2010 31 592 597 10.1086/652778 20402589
7. Predicting the Staphylococcus aureus nasal carrier state: derivation and validation of a culture rule Nouwen JL, Ott A, Kluytmans-Vandenbergh MF, Boelens HA, Hofman A, van Belkum A, Verbrugh HA, Clin Infect Dis 2004 39 806 811 10.1086/423376 15472812 (Pubitemid 39244294)
8. Reclassification of Staphylococcus aureus nasal carriage types. van Belkum A, Verkaik NJ, de Vogel CP, Boelens HA, Verveer J, Nouwen JL, Verbrugh HA, Wertheim HF, J Infect Dis 2009 199 1820 1826 10.1086/599119 19419332
9. Quantification by Real-Time PCR Assay of Staphylococcus aureus Load: a Useful Tool for Rapidly Identifying Persistent Nasal Carriers. Verhoeven PO, Grattard F, Carricajo A, Lucht F, Cazorla C, Garraud O, Pozzetto B, Berthelot P, J Clin Microbiol 2012 50 2063 2065 10.1128/JCM.00157-12 22493331
10. Persistent (not intermittent) nasal carriage of Staphylococcus aureus is the determinant of CPD-related infections. Nouwen JL, Fieren MW, Snijders S, Verbrugh HA, van Belkum A, Kidney Int 2005 67 1084 1092 10.1111/j.1523-1755. 2005.00174.x 15698449 (Pubitemid 41623334)
11. Nasal carriage of Staphylococcus aureus as a major risk factor for wound infections after cardiac surgery. Kluytmans JA, Mouton JW, Ijzerman EP, Vandenbroucke-Grauls CM, Maat AW, Wagenvoort JH, Verbrugh HA, J Infect Dis 1995 171 216 219 10.1093/infdis/171.1.216 7798667
12. Staphylococcus aureus nasal carriage and infection in patients on continuous ambulatory peritoneal dialysis. Luzar MA, Coles GA, Faller B, Slingeneyer A, Dah GD, Briat C, Wone C, Knefati Y, Kessler M, Peluso F, N Engl J Med 1990 322 505 509 10.1056/NEJM199002223220804 2300122 (Pubitemid 20067827)
13. Staphylococcus aureus carriage patterns and the risk of infections associated with continuous peritoneal dialysis. Nouwen J, Schouten J, Schneebergen P, Snijders S, Maaskant J, Koolen M, van Belkum A, Verbrugh HA, J Clin Microbiol 2006 44 2233 2236 10.1128/JCM.02083-05 16757626 (Pubitemid 43902181)
14. Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Wertheim HF, Vos MC, Ott A, van Belkum A, Voss A, Kluytmans JA, van Keulen PH, Vandenbroucke-Grauls CM, Meester MH, Verbrugh HA, Lancet 2004 364 703 705 10.1016/S0140-6736(04)16897-9 15325835 (Pubitemid 39140626)
15. Nasal carriage as a source of Staphylococcus aureus bacteremia. von Eiff C, Becker K, Machka K, Stammer H, Peters G, N Engl J Med 2001 344 11 16 10.1056/NEJM200101043440102 11136954 (Pubitemid 32064839)
16. Preventing surgical-site infections in nasal carriers of Staphylococcus aureus. Bode LG, Kluytmans JA, Wertheim HF, Bogaers D, Vandenbroucke-Grauls CM, Roosendaal R, Troelstra A, Box AT, Voss A, van der Tweel I, van Belkum A, Verbrugh HA, Vos MC, N Engl J Med 2010 362 9 17 10.1056/NEJMoa0808939 20054045
17. Intranasal mupirocin for reduction of Staphylococcus aureus infections in surgical patients with nasal carriage: a systematic review. van Rijen MM, Bonten M, Wenzel RP, Kluytmans JA, J Antimicrob Chemother 2008 61 254 261 18174201
18. Risk factors associated with mupirocin resistance in meticillin-resistant Staphylococcus aureus. Caffrey AR, Quilliam BJ, LaPlante KL, J Hosp Infect 2010 76 206 210 10.1016/j.jhin.2010.06.023 20846747
19. Direct detection of Staphylococcus aureus from adult and neonate nasal swab specimens using real-time polymerase chain reaction. Paule SM, Pasquariello AC, Hacek DM, Fisher AG, Thomson RB, Kaul KL, Peterson LR, J Mol Diagn 2004 6 191 196 10.1016/S1525-1578(10)60509-0 15269294 (Pubitemid 39136645)
20. Evaluation of the IDI-MRSA assay on the SmartCycler real-time PCR platform for rapid detection of MRSA from screening specimens. Rossney AS, Herra CM, Fitzgibbon MM, Morgan PM, Lawrence MJ, OConnell B, Eur J Clin Microbiol Infect Dis 2007 26 459 466 10.1007/s10096-007-0303-7 17541656 (Pubitemid 47025030)
21. Evaluation of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens. Rossney AS, Herra CM, Brennan GI, Morgan PM, OConnell B, J Clin Microbiol 2008 46 3285 3290 10.1128/JCM.02487-07 18685003
22. Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares. Wolk DM, Picton E, Johnson D, Davis T, Pancholi P, Ginocchio CC, Finegold S, Welch DF, de Boer M, Fuller D, Solomon MC, Rogers B, Mehta MS, Peterson LR, J Clin Microbiol 2009 47 758 764 10.1128/JCM.01714-08 19129414
23. Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs. Peterson LR, Liesenfeld O, Woods CW, Allen SD, Pombo D, Patel PA, Mehta MS, Nicholson B, Fuller D, Onderdonk A, J Clin Microbiol 2010 48 1661 1666 10.1128/JCM.00003-10 20335423
24. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Katayama Y, Ito T, Hiramatsu K, Antimicrob Agents Chemother 2000 44 1549 1555 10.1128/AAC.44.6. 1549-1555.2000 10817707 (Pubitemid 30340793)
25. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. Huletsky A, Giroux R, Rossbach V, Gagnon M, Vaillancourt M, Bernier M, Gagnon F, Truchon K, Bastien M, Picard FJ, van Belkum A, Ouellette M, Roy PH, Bergeron MG, J Clin Microbiol 2004 42 1875 1884 10.1128/JCM.42.5.1875- 1884.2004 15131143 (Pubitemid 38637796)
26. Clinical application of real-time PCR to screening critically ill and emergency-care surgical patients for methicillin-resistant Staphylococcus aureus: a quantitative analytical study. Herdman MT, Wyncoll D, Halligan E, Cliff PR, French G, Edgeworth JD, J Clin Microbiol 2009 47 4102 4108 10.1128/JCM.01332-09 19846648
27. Genotypic and phenotypic characterization of methicillin-susceptible Staphylococcus aureus isolates misidentified as methicillin-resistant Staphylococcus aureus by the BD GeneOhm MRSA assay. Stamper PD, Louie L, Wong H, Simor AE, Farley JE, Carroll KC, J Clin Microbiol 2011 49 1240 1244 10.1128/JCM.02220-10 21307210
28. A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay. Bartels MD, Boye K, Rohde SM, Larsen AR, Torfs H, Bouchy P, Skov R, Westh H, J Clin Microbiol 2009 47 1524 1527 10.1128/JCM.02153-08 19297600
29. Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. García-Álvarez L, Holden MT, Lindsay H, Webb CR, Brown DF, Curran MD, Walpole E, Brooks K, Pickard DJ, Teale C, Parkhill J, Bentley SD, Edwards GF, Girvan EK, Kearns AM, Pichon B, Hill RL, Larsen AR, Skov RL, Peacock SJ, Maskell DJ, Holmes MA, Lancet Infect Dis 2011 11 595 603 10.1016/S1473-3099(11) 70126-8 21641281
30. Use of staphylococcal nuclease gene as DNA probe for Staphylococcus aureus. Liebl W, Rosenstein R, Götz F, Schleifer KH, FEMS Microbiol Lett 1987 44 179 184 10.1111/j.1574-6968.1987.tb02264.x
31. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. Brakstad OG, Aasbakk K, Maeland JA, J Clin Microbiol 1992 30 1654 1660 1629319
32. Automated Detection of Toxigenic Clostridium difficile in Clinical Samples: Isothermal tcdB Amplification Coupled to Array-based Detection. Hicke B, Pasko C, Groves B, Ager E, Corpuz M, Frech G, Munns D, Smith W, Warcup A, Denys G, Ledeboer NA, Lindsey W, Owen C, Rea L, Jenison R, J Clin Microbiol 2012 [Epub ahead of print] 23077129
33. Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. An L, Tang W, Ranalli TA, Kim HJ, Wytiaz J, Kong H, J Biol Chem 2005 280 28952 28958 10.1074/jbc.M503096200 15955821 (Pubitemid 41161341)
34. Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus and associated toxin genes. Fosheim GE, Nicholson AC, Albrecht VS, Limbago BM, J Clin Microbiol 2011 49 3071 3073 10.1128/JCM.00795-11 21697325
35. Evaluation of CHROMagar Staph. aureus, a new chromogenic medium, for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens. Gaillot O, Wetsch M, Fortineau N, Berche P, J Clin Microbiol 2000 38 1587 1591 10747148 (Pubitemid 30216694)
36. Sequencing and staphylococci identification. Mellmann A, Becker K, von Eiff C, Keckevoet U, Schumann P, Harmsen D, Emerg Infect Dis 2006 12 333 336 10.3201/eid1202.050962 16494767
37. The human nasal microbiota and Staphylococcus aureus carriage. Frank DN, Feazel LM, Bessesen MT, Price CS, Janoff EN, Pace NR, PLoS One 2010 5 10598 10.1371/journal.pone.0010598 20498722
38. Carriage of Staphylococcus aureus in random samples of a normal population. Noble WC, Valkenburg HA, Wolters CH, J Hyg (Lond) 1967 65 567 573 10.1017/S002217240004609X
39. Childhood epistaxis and nasal colonization with Staphylococcus aureus. Whymark AD, Crampsey DP, Fraser L, Moore P, Williams C, Kubba H, Otolaryngol Head Neck Surg 2008 138 307 310 10.1016/j.otohns.2007.10.029 18312876 (Pubitemid 351288429)
40. Hemoglobin Promotes Staphylococcus aureus Nasal Colonization. Pynnonen M, Stephenson RE, Schwartz K, Hernandez M, Boles BR, PLoS Pathog 2011 7 1002104 10.1371/journal.ppat.1002104 21750673