Non-native aggregation of recombinant human granulocyte-colony stimulating factor under simulated process stress conditions

Biotechnology Journal

Volumn 7, Issue 8, 2012, Pages 1014-1024

  Roessl, Ulrich ^a,b   Wiesbauer, Johanna ^a,b   Leitgeb, Stefan ^a,b   Birner Gruenberger, Ruth ^c   Nidetzky, Bernd ^a,b  

^a RESEARCH CENTER PHARMACEUTICAL ENGINEERING GMBH   (Austria)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^c MEDICAL UNIVERSITY OF GRAZ   (Austria)

Author keywords

Granulocyte colony stimulating factor; Process conditions; Protein aggregation; Protein pharmaceuticals

Indexed keywords

CIRCULAR SHAPE; CONFOCAL LASER SCANNING MICROSCOPY; CROSSLINKS; DISULFIDE BONDS; GRANULOCYTE-COLONY STIMULATING FACTOR; HYDROPHOBIC SURFACES; INCUBATION TIME; INTRAMOLECULAR DISULFIDE BONDS; NON-NATIVE; PROCESS CONDITION; PRODUCT QUALITY; PRODUCTION PROCESS; PROTEIN AGGREGATION; PROTEIN PHARMACEUTICALS; SIMULATED PROCESS; SOLUBLE AGGREGATES; STRESS CONDITION; TIME-RESOLVED;

AGGLOMERATION; AMINO ACIDS; CONFOCAL MICROSCOPY; COVALENT BONDS; ESCHERICHIA COLI; MASS SPECTROMETRY; RECOMBINANT PROTEINS; SURFACE CHEMISTRY;

AGGREGATES;

CYSTEINE; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; THIOL DERIVATIVE;

ADDITION REACTION; AERATION; ARTICLE; CONFOCAL LASER MICROSCOPY; DISULFIDE BOND; HYDROPHOBICITY; MASS SPECTROMETRY; MECHANICAL STRESS; MOLECULAR SIZE; PRECIPITATION; PRIORITY JOURNAL; PROCESS MODEL; PROCESS OPTIMIZATION; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN CROSS LINKING; PROTEIN DENATURATION; PROTEIN STABILITY; PROTEIN STRUCTURE; REDUCTION; SIMULATION; STRUCTURE ANALYSIS; SURFACE PROPERTY;

BIOREACTORS; BIOTECHNOLOGY; DISULFIDES; GRANULOCYTE COLONY-STIMULATING FACTOR; HUMANS; MICROSCOPY, CONFOCAL; MODELS, CHEMICAL; MODELS, MOLECULAR; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 84864803427     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201100436     Document Type: Article

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