Effect of triethylene glycol dimethacrylate on the cytotoxicity, cyclooxygenase-2 expression and prostanoids production in human dental pulp cells

International Endodontic Journal

Volumn 45, Issue 9, 2012, Pages 848-858

  Chang, H H ^a   Chang, M C ^a   Huang, G F ^a   Wang, Y L ^a   Chan, C P ^b   Wang, T M ^a   Lin, P S ^a   Jeng, J H ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b CHANG GUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Cell cycle; Cyclooxygenase; Dental pulp cells; Prostaglandin; Resin monomers; Triethylene glycol dimethacrylate

Indexed keywords

CCNB1 PROTEIN, HUMAN; CDC2 PROTEIN, HUMAN; CDC25C PROTEIN, HUMAN; CDKN1A PROTEIN, HUMAN; COLORING AGENT; CYCLIN B; CYCLIN B1; CYCLIN DEPENDENT KINASE INHIBITOR 1A; CYCLOOXYGENASE 2; DENTAL MATERIAL; DIAGNOSTIC AGENT; ENZYME INHIBITOR; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; LIPOCORTIN 5; MACROGOL DERIVATIVE; POLYMETHACRYLIC ACID DERIVATIVE; PROPIDIUM IODIDE; PROSTAGLANDIN; PROSTAGLANDIN E2; PROSTAGLANDIN F2 ALPHA; PROTEIN TYROSINE PHOSPHATASE; PTGS2 PROTEIN, HUMAN; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE; TRIETHYLENE GLYCOL DIMETHACRYLATE;

APOPTOSIS; ARTICLE; BIOSYNTHESIS; CELL CYCLE; CELL DEATH; CELL PROLIFERATION; CELL SHAPE; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; FLOW CYTOMETRY; HUMAN; METHODOLOGY; NECROSIS; TIME; TOOTH PULP;

ANNEXIN A5; APOPTOSIS; CDC25 PHOSPHATASES; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL DEATH; CELL PROLIFERATION; CELL SHAPE; COLORING AGENTS; CYCLIN B; CYCLIN B1; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLOOXYGENASE 2; DENTAL MATERIALS; DENTAL PULP; DINOPROST; DINOPROSTONE; ENZYME INHIBITORS; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HUMANS; NECROSIS; POLYETHYLENE GLYCOLS; POLYMETHACRYLIC ACIDS; PROPIDIUM; PROSTAGLANDINS; TETRAZOLIUM SALTS; THIAZOLES; TIME FACTORS;

EID: 84864800868     PISSN: 01432885     EISSN: 13652591     Source Type: Journal    
DOI: 10.1111/j.1365-2591.2012.02042.x     Document Type: Article

References (51)

1. Abraham RT (2001) Cell cycle checkpoint signaling through the ATM and ATR kinases. Genes & Development 15, 2177-2196.
2. Accorinte ML, Loguercio AD, Reis A, Costa CA (2008) Response of human pulps capped with different self-etch adhesive systems. Clinical Oral Investigations 12, 119-127.
3. Alptekin NO, Ari H, Haliloglu S, Alptekin T, Serpek B, Ataoglu T (2005) The effect of endodontic therapy on periapical exudate neutrophil elastase and prostaglandin-E2 levels. Journal of Endodontics 31, 791-795.
4. Bakopoulou A, Papadopoulos T, Garefis P (2009) Molecular toxicology of substances released from resin-based dental restorative materials. International Journal of Molecular Science 10, 3861-3899.
5. Basu S (2007) Novel cyclooxygenase-catalyzed bioactive prostaglandin F2alpha from physiology to new principles in inflammation. Medical Research Review 27, 435-468.
6. Chang YC, Yang SF, Huang FM, Liu CM, Tai KW, Hsieh YS (2003) Proinflammatory cytokines induce cyclooxygenase-2 mRNA and protein expression in human pulp cell cultures. Journal of Endodontics 29, 201-204.
7. Chang MC, Wu HL, Lee JJ et al. (2004) The induction of prostaglandin E2 production, interleukin-6 production, cell cycle arrest, and cytotoxicity in primary oral keratinocytes and KB cancer cells by areca nut ingredients is differentially regulated by MEK/ERK activation. Journal of Biological Chemistry 279, 50676-50683.
8. Chang HH, Guo MK, Kasten FH et al. (2005) Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 26, 745-753.
9. Chang MC, Chen YJ, Tai TF et al. (2006) Cytokine-induced prostaglandin E2 production and cyclooxygenase-2 expression in dental pulp cells: downstream calcium signalling via activation of prostaglandin EP receptor. International Endodontic Journal 39, 819-826.
10. Chang MC, Lin LD, Chan CP et al. (2009a) The effect of BisGMA on cyclooxygenase-2 expression, PGE2 production and cytotoxicity via reactive oxygen species- and MEK/ERK-dependent and -independent pathways. Biomaterials 30, 4070-4077.
11. Chang MC, Chang HH, Lee MY et al. (2009b) Prostaglandin F(2alpha)-induced interleukin-8 production in human dental pulp cells is associated with MEK/ERK signaling. Journal of Endodontics 35, 508-512.
12. Chang MC, Chen LI, Chan CP et al. (2010a) The role of reactive oxygen species and hemeoxygenase-1 expression in the cytotoxicity, cell cycle alteration and apoptosis of dental pulp cells induced by BisGMA. Biomaterials 31, 8164-8171.
13. Chang MC, Chen YJ, Lee MY et al. (2010b) Prostaglandin F(2alpha) stimulates MEK-ERK signalling but decreases the expression of alkaline phosphatase in dental pulp cells. International Endodontic Journal 43, 461-468.
14. Cohen JS, Reader A, Fertel R, Beck M, Meyers WJ (1985) A radioimmunoassay determination of the concentrations of prostaglandins E2 and F2alpha in painful and asymptomatic human dental pulps. Journal of Endodontics 11, 330-335.
15. Eckhardt A, Harorli T, Limtanyakul J et al. (2009) Inhibition of cytokine and surface antigen expression in LPS-stimulated murine macrophages by triethylene glycol dimethacrylate. Biomaterials 30, 1665-1674.
16. Eckhardt A, Muller P, Hiller KA et al. (2010) Influence of TEGDMA on the mammalian cell cycle in comparison with chemotherapeutic agents. Dental Materials 26, 232-241.
17. Engelmann J, Leyhausen G, Leibfritz D, Geurtsen W (2001) Metabolic effects of dental resin components in vitro detected by NMR spectroscopy. Journal of Dental Research 80, 869-875.
18. Engelmann J, Volk J, Leyhausen G, Geurtsen W (2005) ROS formation and glutathione levels in human oral fibroblasts exposed to TEGDMA and camphorquinone. Journal of Biomedical Material Research. Part B, Applied Biomaterials 75, 272-276.
19. Feng L, Sun W, Xia Y et al. (1993) Cloning two isoforms of rat cyclooxygenase: differential regulation of their expression. Archieves Biochemistry Biophysics 307, 361-368.
20. Galler KM, Schweikl H, Hiller KA et al. (2011) TEGDMA reduces mineralization in dental pulp cells. Journal of Dental Research 90, 257-262.
21. Gautier J, Minshull J, Lohka M, Glotzer M, Hunt T, Maller JL (1990) Cyclin is a component of maturation-promoting factor from Xenopus. Cell 60, 487-494.
22. Gerzina TM, Hume WR (1996) Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. Journal of Dentistry 24, 125-128.
23. Geurtsen W, Leyhausen G (2001) Chemical-biological interactions of the resin monomer triethyleneglycol-dimethacrylate (TEGDMA). Journal of Dental Research 80, 2046-2050.
24. Guven G, Altun C, Gunhan O et al. (2007) Co-expression of cyclooxygenase-2 and vascular endothelial growth factor in inflamed human pulp: an immunohistochemical study. Journal of Endodontics 33, 18-20.
25. Hafez AA, Cox CF, Tarim B, Otsuki M, Akimoto N (2002) An in vivo evaluation of hemorrhage control using sodium hypochlorite and direct capping with a one- or two-component adhesive system in exposed nonhuman primate pulps. Quintessence International 33, 261-272.
26. Hebling J, Giro EM, Costa CA (1999) Biocompatibility of an adhesive system applied to exposed human dental pulp. Journal of Endodontics 25, 676-682.
27. Jeng JH, Ho YS, Chan CP et al. (2000) Areca nut extract up-regulates prostaglandin production, cyclooxygenase-2 mRNA and protein expression of human oral keratinocytes. Carcinogenesis 21, 1365-1370.
28. Kleinsasser NH, Schmid K, Sassen AW et al. (2006) Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay. Biomaterials 27, 1762-1770.
29. Krifka S, Petzel C, Hiller KA et al. (2010) Resin monomer-induced differential activation of MAP kinases and apoptosis in mouse macrophages and human pulp cells. Biomaterials 31, 2964-2975.
30. Lee DH, Kim NR, Lim BS, Lee YK, Yang HC (2009) Effects of TEGDMA and HEMA on the expression of COX-2 and iNOS in cultured murine macrophage cells. Dental Materials 25, 240-246.
31. Legler DF, Bruckner M, Uetz-von Allmen E, Krause P (2010) Prostaglandin E2 at new glance: novel insights in functional diversity offer therapeutic chances. International Journal of Biochemistry & Cell Biology 42, 198-201.
32. Lin SK, Kuo MY, Wang JS et al. (2002) Differential regulation of interleukin-6 and inducible cyclooxygenase gene expression by cytokines through prostaglandin-dependent and -independent mechanisms in human dental pulp fibroblasts. Journal of Endodontics 28, 197-201.
33. Lukas J, Lukas C, Bartek J (2004) Mammalian cell cycle checkpoints: signalling pathways and their organization in space and time. DNA Repair 3, 997-1007.
34. Maity A, McKenna WG, Muschel RJ (1994) The molecular basis for cell cycle delays following ionizing radiation: a review. Radiotherapeutic Oncology 31, 1-13.
35. Maltos KL, Menezes GB, Caliari MV et al. (2004) Vascular and cellular responses to pro-inflammatory stimuli in rat dental pulp. Archieves of Oral Biology 49, 443-450.
36. Milatovic D, Montine TJ, Aschner M (2011) Prostanoid signaling: dual role for prostaglandin E2 in neurotoxicity. Neurotoxicology 32, 312-319.
37. Miyauchi M, Takata T, Ito H et al. (1996) Immunohistochemical demonstration of prostaglandins E2, F2 alpha, and 6-keto-prostaglandin F1 alpha in rat dental pulp with experimentally induced inflammation. Journal of Endodontics 22, 600-602.
38. Moore A, Donahue CJ, Bauer KD, Mather JP (1998) Simultaneous measurement of cell cycle and apoptotic cell death. Methods in Cell Biology 57, 265-278.
39. Nakanishi T, Shimizu H, Hosokawa Y, Matsuo T (2001) An immunohistological study on cyclooxygenase-2 in human dental pulp. Journal of Endodontics 27, 385-388.
40. Schweikl H, Altmannberger I, Hanser N et al. (2005) The effect of triethylene glycol dimethacrylate on the cell cycle of mammalian cells. Biomaterials 26, 4111-4118.
41. Schweikl H, Spagnuolo G, Schmalz G (2006) Genetic and cellular toxicology of dental resin monomers. Journal of Dental Research 85, 870-877.
42. Silva GA, Lanza LD, Lopes-Junior N, Moreira A, Alves JB (2006) Direct pulp capping with a dentin bonding system in human teeth: a clinical and histological evaluation. Operative Dentistry 31, 297-307.
43. Spagnuolo G, Galler K, Schmalz G, Cosentino C, Rengo S, Schweikl H (2004) Inhibition of phosphatidylinositol 3-kinase amplifies TEGDMA-induced apoptosis in primary human pulp cells. Journal of Dental Research 83, 703-707.
44. Tai TF, Chan CP, Lin CC, Chen LI, Jeng JH, Chang MC (2008) Transforming growth factor beta2 regulates growth and differentiation of pulp cells via ALK5/Smad2/3. Journal of Endodontics 34, 427-432.
45. Taylor WR, Stark GR (2001) Regulation of the G2/M transition by p53. Oncogene, 20, 1803-1815.
46. Tseng SK, Chang MC, Su CY et al. (2011) Arecoline induced cell cycle arrest, apoptosis, and cytotoxicity to human endothelial cells. Clinical Oral Investigation (In Press).
47. Tziafas D, Koliniotou-Koumpia E, Tziafa C, Papadimitriou S (2007) Effects of a new antibacterial adhesive on the repair capacity of the pulp-dentine complex in infected teeth. International Endodontic Journal 40, 58-66.
48. Vermeulen K, Van Bockstaele DR, Berneman ZN (2003) The cell cycle: a review of regulation, deregulation and therapeutic targets in cancer. Cell Proliferation 36, 131-149.
49. Waterhouse PJ, Nunn JH, Whitworth JM (2002) Prostaglandin E2 and treatment outcome in pulp therapy of primary molars with carious exposures. International Journal of Paediatric Dentistry 12, 116-123.
50. Wisniewska-Jarosinska M, Poplawski T, Chojnacki CJ et al. (2011) Independent and combined cytotoxicity and genotoxicity of triethylene glycol dimethacrylate and urethane dimethacrylate. Molecular Biology Report 38, 4603-4611.
51. Yeh HW, Chang MC, Lin CP et al. (2009) Comparative cytotoxicity of five current dentin bonding agents: role of cell cycle deregulation. Acta Biomaterialia 5, 3404-3410.