Metabolic imaging using two-photon excited nadh intensity and fluorescence lifetime imaging

Microscopy and Microanalysis

Volumn 18, Issue 4, 2012, Pages 761-770

  Vergen, Jorge ^a   Hecht, Clifford ^a   Zholudeva, Lyandysha V ^a   Marquardt, Meg M ^a   Hallworth, Richard ^b   Nichols, Michael G ^a  

^a CREIGHTON UNIVERSITY   (United States)

^b CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

fluorescence; lifetime imaging; multiphoton microscopy; NADH

Indexed keywords

NICOTINAMIDE ADENINE DINUCLEOTIDE;

ANIMAL; CELL LINE; CELLS; CHEMISTRY; CONFERENCE PAPER; CYTOLOGY; EVALUATION; KINETICS; METABOLISM; METHODOLOGY; MITOCHONDRION; MULTIPHOTON MICROSCOPY; RAT;

ANIMALS; CELL LINE; CELLS; KINETICS; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; MITOCHONDRIA; NAD; RATS;

EID: 84864757468     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927612000529     Document Type: Conference Paper

References (33)

1. Agronskaia, A.V., Tertoolen, L. & Gerritsen, H.C. (2004). Fast fluorescence lifetime imaging of calcium in living cells. J Biomed Opt 9, 1230-1237.
2. An, J., Camara, A.K., Rhodes, S.S., Riess, M.L. & Stowe, D.F. (2005). Warm ischemic preconditioning improves mitochondrial redox balance during and after mild hypothermic ischemia in guinea pig isolated hearts. Am J Physiol Heart Circ Physiol 288, H2620-H2627.
3. Belke, D.D., Larsen, T.S., Gibbs, E.M. & Severson, D.L. (2000). Altered metabolism causes cardiac dysfunction in perfused hearts from diabetic (db/db) mice. Am J Physiol Endocrinol Metab 279(5), E1104-13.
4. Bevington, P.R. & Robinson, D.K. (2002). Data Reduction and Error Analysis for the Physical Sciences. New York: McGraw-Hill.
5. Bird, D.K., Yan, L., Vrotsos, K.M., Eliceiri, K.W., Vaughan, E.M., Keely, P.J.,White, J.G. & Ramanujam, N. (2005). Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH. Cancer Res 65, 8766-8773.
6. Blinova, K., Carroll, S., Bose, S., Smirnov, A.V., Harvey, J.J., Knutson, J.R. & Balaban, R.S. (2005). Distribution of mitochondrial NADH fluorescence lifetimes: Steady-state kinetics of matrix NADH interactions. Biochemistry 44, 2585-2594.
7. Blinova, K., Combs, C., Kellman, P. & Balaban, R.S. (2004). Fluctuation analysis of mitochondrial NADH fluorescence signals in confocal and two-photon microscopy images of living cardiac myocytes. J Microsc 213, 70-75.
8. Chance, B. & Baltscheffsky, H. (1958). Respiratory enzymes in oxidative phosphorylation. VII. Binding of intramitochondrial reduced pyridine nucleotide. J Biol Chem 233, 736-739.
9. Chance, B. & Lieberman, M. (1978). Intrinsic fluorescence emission from the cornea at low temperatures: Evidence of mitochondrial signals and their differing redox states in epithelial and endothelial sides. Exp Eye Res 26, 111-117.
10. Chance, B., Oshino, N., Sugano, T. & Mayevsky, A. (1973). Basic principles of tissue oxygen determination from mitochondrial signals. Adv Exp Med Biol 37A, 277-292.
11. Costello, L.C. & Franklin, R.B. (2006). Tumor cell metabolism: The marriage of molecular genetics and proteomics with cellular intermediary metabolism; proceed with caution) Mol Cancer 5, 59.
12. Evans, N.D., Gnudi, L., Rolinski, O.J., Birch, D.J. & Pickup, J.C. (2005). Glucose-dependent changes in NAD(P)H-related fluorescence lifetime of adipocytes and fibroblasts in vitro: Potential for non-invasive glucose sensing in diabetes mellitus. J Photochem Photobiol B 80, 122-129.
13. Huang, S., Heikal, A.A. & Webb, W.W. (2002). Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein. Biophys J 82, 2811-2825.
14. Indig, G.L., Anderson, G.S., Nichols, M.G., Bartlett, J.A., Mellon,W.S. & Sieber, F. (2000). Effect of molecular structure on the performance of triarylmethane dyes as therapeutic agents for photochemical purging of autologous bone marrow grafts from residual tumor cells. J Pharm Sci 89, 88-99.
15. Kasischke, K.A., Vishwasrao, H.D., Fisher, P.J., Zipfel, W.R. & Webb, W.W. (2004). Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis. Science 305, 99-103.
16. Lakowicz, J.R., Szmacinski, H., Nowaczyk, K. & Johnson, M.L. (1992). Fluorescence lifetime imaging of free and proteinbound NADH. Proc Natl Acad Sci USA 89, 1271-1275.
17. Levene, M.J., Dombeck, D.A., Kasischke, K.A., Molloy, R.P. & Webb, W.W. (2004). In vivo multiphoton microscopy of deep brain tissue. J Neurophysiol 91, 1908-1912.
18. Lopaschuk, G.D., Folmes, C.D. & Stanley,W.C. (2007). Cardiac energy metabolism in obesity. Circ Res 101(4), 335-347.
19. Mayevsky, A. & Rogatsky, G.G. (2007). Mitochondrial function in vivo evaluated by NADH fluorescence: From animal models to human studies. Am J Physiol Cell Physiol 292, C615-C640.
20. Nichols, M.G., Barth, E.E. & Nichols, J.A. (2005). Reduction in DNA synthesis during two-photon microscopy of intrinsic reduced nicotinamide adenine dinucleotide fluorescence. Photochem Photobiol 81, 259-269.
21. Nichols, M.G. & Webb, W.W. (1998). Simultaneous imaging of photofrin and NADH autofluorescence in cell monolayers and multicell tumor spheroids. Photochem Photobiol 67S, 95S.
22. Piston, D.W., Masters, B.R. & Webb, W.W. (1995). Threedimensionally resolved NAD(P)H cellular metabolic redox imaging of the in situ cornea with two-photon excitation laser scanning microscopy. J Microsc 178(Pt 1), 20-27.
23. Rocheleau, J.V., Head,W.S. & Piston, D.W. (2004). Quantitative NAD(P)H/flavoprotein autofluorescence imaging reveals metabolic mechanisms of pancreatic islet pyruvate response. J Biol Chem 279, 31780-31787.
24. Romashko, D.N., Marban, E. & O'Rourke, B. (1998). Subcellular metabolic transients and mitochondrial redox waves in heart cells. Proc Natl Acad Sci USA 95, 1618-1623.
25. Scott, T.G., Spencer, R.D., Leonard, N.J. & Weber, G. (1970). Synthetic spectroscopic models related to coenzymes and base pairs. V. Emission properties of NADH. studies of fluorescence lifetimes and quantum efficiencies of NADH, AcPyADH, [reduced acetylpyridineadenine dinucleotide] and simplified synthetic models. J Am Chem Soc 92, 687-695.
26. Skala, M.C., Riching, K.M., Bird, D.K., Gendron-Fitzpatrick, A., Eickhoff, J., Eliceiri, K.W., Keely, P.J. & Ramanujam, N. (2007a). In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia. J Biomed Opt 12, 024014.
27. Skala, M.C., Riching, K.M., Gendron-Fitzpatrick, A., Eickhoff, J., Eliceiri, K.W., White, J.G. & Ramanujam, N. (2007b). In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia. Proc Natl Acad Sci USA 104, 19494-19499.
28. Tiede, L.M. & Nichols, M.G. (2006). Photobleaching of reduced nicotinamide adenine dinucleotide and the development of highly fluorescent lesions in rat basophilic leukemia cells during multiphoton microscopy. Photochem Photobiol 82, 656-664.
29. Tiede, L.M., Rocha-Sanchez, S.M., Hallworth, R., Nichols, M.G. & Beisel, K. (2007). Determination of hair cell metabolic state in isolated cochlear preparations by two-photon microscopy. J Biomed Opt 12, 021004.
30. Vishwasrao, H.D., Heikal, A.A., Kasischke, K.A. &Webb,W.W. (2005). Conformational dependence of intracellular NADH on metabolic state revealed by associated fluorescence anisotropy. J Biol Chem 280, 25119-25126.
31. Wallace, D.C. (2005). A mitochondrial paradigm of metabolic and degenerative diseases, aging, and cancer: A dawn for evolutionary medicine. Annu Rev Genet 39, 359-407.
32. Yu, Q. & Heikal, A.A. (2009). Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the singlecell level. J Photochem Photobiol B 95, 46-57.
33. Zipfel, W.R., Williams, R.M., Christie, R., Nikitin, A.Y., Hyman, B.T. & Webb, W.W. (2003). Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci USA 100, 7075-7080.