A targeted gene knockout method using a newly constructed temperature-sensitive plasmid mediated homologous recombination in Bifidobacterium longum

Applied Microbiology and Biotechnology

Volumn 95, Issue 2, 2012, Pages 499-509

  Sakaguchi, Kouta ^a   He, Jianlong ^a   Tani, Saori ^a   Kano, Yasunobu ^b   Suzuki, Tohru ^a  

^a GIFU UNIVERSITY   (Japan)

^b KYOTO PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

Bifidobacteria; Gene knockout; Homologous recombination; Mutagenesis; Temperature sensitive plasmid

Indexed keywords

BIFIDOBACTERIA; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM LONGUM; DNA SEQUENCE ANALYSIS; ERROR PRONE PCR; GENE KNOCKOUT; GENE-KNOCKOUT EXPERIMENTS; HOMOLOGOUS RECOMBINATION; HOMOLOGOUS SEQUENCES; MICROFLORA; MUTANT PLASMIDS; RANDOM MUTAGENESIS; RECOMBINATION FREQUENCIES; SHUTTLE VECTOR; SITE DIRECTED MUTAGENESIS; TEMPERATURE-SENSITIVE;

BACTERIA; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPERIMENTS; GENES; MUTAGENESIS; POLYMERASE CHAIN REACTION;

DNA;

BACTERIOLOGY; BACTERIUM; BIOTECHNOLOGY; GENE EXPRESSION; HOMOLOGY; MUTATION; PHENOTYPE; PLASMID; RECOMBINATION; TEMPERATURE EFFECT;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM MUTANT; BIFIDOBACTERIUM LONGUM; DNA SEQUENCE; GENE INACTIVATION; HOMOLOGOUS RECOMBINATION; MISSENSE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; SITE DIRECTED MUTAGENESIS; TEMPERATURE SENSITIVITY;

BACTERIAL PROTEINS; BIFIDOBACTERIUM; DNA REPLICATION; GENE KNOCKOUT TECHNIQUES; GENES, BACTERIAL; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; MUTATION, MISSENSE; PLASMIDS; TEMPERATURE;

BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM LONGUM; ESCHERICHIA COLI;

EID: 84864706348     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4090-4     Document Type: Article

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