Targeting the Bacillus subtilis genome: An efficient and clean method for gene disruption

Journal of Microbiological Methods

Volumn 70, Issue 3, 2007, Pages 389-394

  Sanchez, Humberto ^a   Cozar, M Castillo ^a   Martinez Jimenez, Maria I ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Bacillus subtilis mutants; Gene disruption; Site specific recombination

Indexed keywords

CHLORAMPHENICOL; RECOMBINASE; SPECTINOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BACTERIAL CHROMOSOME; BACTERIAL GENOME; CATALYSIS; ENZYME ACTIVE SITE; GENE AMPLIFICATION; GENE CASSETTE; GENE DISRUPTION; GENE SEQUENCE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION;

BACILLUS SUBTILIS; GENOME, BACTERIAL; MUTAGENESIS, INSERTIONAL; PLASMIDS; RECOMBINATION, GENETIC; TRANSFORMATION, BACTERIAL;

BACILLUS SUBTILIS;

EID: 34547720649     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.05.004     Document Type: Article

References (23)

1. Alonso J.C., Stiege A.C., and Luder G. Genetic recombination in Bacillus subtilis 168: effect of recN, recF, recH and addAB mutations on DNA repair and recombination. Mol. Gen. Genet. 239 (1993) 129-136
2. Alonso J.C., Weise F., and Rojo F. The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the beta recombinase of plasmid pSM19035. J. Biol. Chem. 270 (1995) 2938-2945
3. Ayora S., Rojo F., Ogasawara N., Nakai S., and Alonso J.C. The Mfd protein of Bacillus subtilis 168 is involved in both transcription-coupled DNA repair and DNA recombination. J. Mol. Biol. 256 (1996) 301-318
4. Campo N., Daveran-Mingot M.L., Leenhouts K., Ritzenthaler P., and Le Bourgeois P. Cre-loxP recombination system for large genome rearrangements in Lactococcus lactis. Appl. Environ. Microbiol. 68 (2002) 2359-2367
5. Canosa I., Rojo F., and Alonso J.C. Site-specific recombination by the beta protein from the streptococcal plasmid pSM19035: minimal recombination sequences and crossing over site. Nucleic Acids Res. 24 (1996) 2712-2717
6. Ceglowski P., Boitsov A., Chai S., and Alonso J.C. Analysis of the stabilization system of pSM19035-derived plasmid pBT233 in Bacillus subtilis. Gene 136 (1993) 1-12
7. Ceglowski P., Boitsov A., Karamyan N., Chai S., and Alonso J.C. Characterization of the effectors required for stable inheritance of Streptococcus pyogenes pSM19035-derived plasmids in Bacillus subtilis. Mol. Gen. Genet. 241 (1993) 579-585
8. Datsenko K.A., and Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 6640-6645
9. Fernandez S., Sorokin A., and Alonso J.C. Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination. J. Bacteriol. 180 (1998) 3405-3409
10. Itaya M., and Tanaka T. Experimental surgery to create subgenomes of Bacillus subtilis 168. Proc. Natl. Acad. Sci. U. S. A. 94 (1997) 5378-5382
11. Khasanov F.K., Zvingila D.J., Zainullin A.A., Prozorov A.A., and Bashkirov V.I. Homologous recombination between plasmid and chromosomal DNA in Bacillus subtilis requires approximately 70 bp of homology. Mol. Gen. Genet. 234 (1992) 494-497
12. Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.K., Codani J.J., Connerton I.F., Danchin A., et al. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390 (1997) 249-256
13. Lambert J.M., Bongers R.S., and Kleerebezem M. Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum. Appl. Environ. Microbiol. 73 (2007) 1126-1135
14. Rojo F., and Alonso J.C. A novel site-specific recombinase encoded by the Streptococcus pyogenes plasmid pSM19035. J. Mol. Biol. 238 (1994) 159-172
15. Rojo F., and Alonso J.C. The beta recombinase from the Streptococcal plasmid pSM 19035 represses its own transcription by holding the RNA polymerase at the promoter region. Nucleic Acids Res. 22 (1994) 1855-1860
16. Rottlander E., and Trautner T.A. Genetic and transfection studies with B. subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168. Mol. Gen. Genet. 108 (1970) 47-60
17. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: a Laboratory Manual (1989), Cold Spring Harbor laboratory Press, New York
18. Sanchez H., Kidane D., Reed P., Curtis F.A., Cozar M.C., Graumann P.L., Sharples G.J., and Alonso J.C. The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis. Genetics 171 (2005) 873-883
19. Sanchez H., Kidane D., Castillo Cozar M., Graumann P.L., and Alonso J.C. Recruitment of Bacillus subtilis RecN to DNA double-strand breaks in the absence of DNA end processing. J. Bacteriol. 188 (2006) 353-360
20. Sanchez, H., Carrasco, B., Cozar, M.C., Alonso, J.C., in press. Bacillus subtilis RecG branch migration translocase is required for DNA repair and chromosomal segregation. Mol. Microbiol.
21. Shaw W.V., Brenner D.G., LeGrice S.F., Skinner S.E., and Hawkins A.R. Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221. Nucleotide sequence analysis and expression studies. FEBS Lett. 179 (1985) 101-106
22. Toda T., Tanaka T., and Itaya M. A method to invert DNA segments of the Bacillus subtilis 168 genome by recombination between two homologous sequences. Biosci. Biotechnol. Biochem. 60 (1996) 773-778
23. Vagner V., Dervyn E., and Ehrlich S.D. A vector for systematic gene inactivation in Bacillus subtilis. Microbiology 144 Pt 11 (1998) 3097-3104