Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin

Biochemistry

Volumn 51, Issue 31, 2012, Pages 6164-6170

  Petrillo, Teodolinda ^a   O'Donohoe, Catrina A ^a   Howe, Nicole ^a   Malthouse, J Paul G ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; CARBOXYL GROUPS; CARBOXYLATE GROUPS; CATALYTIC EFFICIENCIES; CATALYTIC MECHANISMS; GENERAL BASE; GLYOXAL; HYDROXYL GROUPS; INTERMEDIATE FORMATION; LOW-BARRIER HYDROGEN BONDS; MOLARITY; OXYANIONS; SERINE PROTEASE; SUBSTRATE BINDING; SUBTILISIN; SUBTILISIN CARLSBERG; TETRAHEDRAL INTERMEDIATES;

CARBOXYLATION; CATALYST ACTIVITY; HYDROGEN BONDS;

AMINO ACIDS;

ASPARTIC ACID; CARBOXYL GROUP; CARBOXYLIC ACID; CHYMOTRYPSIN A; HISTIDINE; SERINE PROTEINASE; SUBTILISIN;

ARTICLE; BINDING KINETICS; BIOCATALYST; CATALYSIS; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; HYDROGEN BOND; INHIBITION KINETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; PROTEIN STABILITY; PROTEIN SYNTHESIS; SIGNAL TRANSDUCTION;

BIOCATALYSIS; CATALYTIC DOMAIN; CHYMOTRYPSIN; GLYOXAL; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; OLIGOPEPTIDES; PROTEASE INHIBITORS; SERINE; SUBTILISIN;

EID: 84864684042     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300688k     Document Type: Article

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