Determination of the structure of tetrahedral transition state analogues bound at the active site of chymotrypsin using 18O and 2H isotope shifts in the 13C NMR spectra of glyoxal inhibitors

Biochemistry

Volumn 46, Issue 44, 2007, Pages 12868-12874

  Spink, Edward ^a   Hewage, Chandralal ^a   Malthouse, J Paul G ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

BENZYLOXYCARBONYL; EXCHANGEABLE OXYGEN ATOMS; GLYOXAL INHIBITORS; ISOTOPE SHIFTS;

CARBON; HYDRATES; ISOTOPES; NUCLEAR MAGNETIC RESONANCE; NUCLEOPHILES; PEPTIDES;

ENZYME ACTIVITY;

ALDEHYDE; BENZYLOXYCARBONYLALANYLPROLYLPHENYLALANYLGLYOXAL; CHYMOTRYPSIN; CHYMOTRYPSIN A; CYSTEINE PROTEINASE; DEUTERIUM; GLYOXAL; HYDROXYL GROUP; INHIBITOR PROTEIN; OXYGEN 18; PEPTIDE; SERINE; SERINE PROTEINASE; UNCLASSIFIED DRUG; WATER;

ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME SUBSTRATE COMPLEX; ISOTOPE ANALYSIS; PRIORITY JOURNAL; REACTION ANALYSIS; STRUCTURE ANALYSIS;

BINDING SITES; CHYMOTRYPSIN; DEUTERIUM; DEUTERIUM EXCHANGE MEASUREMENT; GLYOXAL; MODELS, BIOLOGICAL; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OLIGOPEPTIDES; OXYGEN ISOTOPES; PROTEIN BINDING;

EID: 35848969800     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi701212u     Document Type: Article

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