Biacore surface matrix effects on the binding kinetics and affinity of an antigen/antibody complex

Analytical Biochemistry

Volumn 429, Issue 1, 2012, Pages 58-69

  Drake, Andrew W ^a,b   Tang, Margaret L ^a   Papalia, Giuseppe A ^a,c   Landes, Gregory ^a,d   Haak Frendscho, Mary ^a,b   Klakamp, Scott L ^a,e  

^a Takeda San Francisco   (United States)

^b Compugen Inc ^*  (United States)

^c GILEAD SCIENCES   (United States)

^d DNA Bridges Inc   (United States)

^e SKD Consulting LLC   (United States)

Author keywords

Affinity; Antigen antibody complexes; Biacore; Equilibrium dissociation constants; Kinetic exclusion assay (KinExA); Kinetic rate constants; Negatively charged dextran layer; SPR; Surface plasmon resonance

Indexed keywords

ANTIGENS; DISSOCIATION; EQUILIBRIUM CONSTANTS; MONOCLONAL ANTIBODIES; RATE CONSTANTS; SURFACE PLASMON RESONANCE;

AFFINITY; ANTIGEN-ANTIBODY COMPLEX; BIACORE; EQUILIBRIUM DISSOCIATION CONSTANT; KINETIC RATE CONSTANTS; NEGATIVELY CHARGED;

KINETICS;

CARBOXYL GROUP; CARBOXYMETHYLDEXTRAN; MONOCLONAL ANTIBODY;

ANTIBODY TITER; ANTIGEN ANTIBODY COMPLEX; ARTICLE; ARTIFACT; BINDING AFFINITY; BINDING KINETICS; BIOASSAY; BIOCHIP; CONTROLLED STUDY; DIAGNOSTIC TEST; OPTICAL BIOSENSOR; PRIORITY JOURNAL; STATIC ELECTRICITY;

EID: 84864541676     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.06.024     Document Type: Article

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