Counterion effects on protein adsorption equilibrium and kinetics in polymer-grafted cation exchangers

Journal of Chromatography A

Volumn 1253, Issue , 2012, Pages 83-93

  Almodovar, Ernie X Perez ^a,b   Glatz, Bianca ^a   Carta, Giorgio ^a  

^a University of Virginia   (United States)

^b Amgen Manufacturing Limited   (United States)

Author keywords

Counterions; Equilibrium; Grafted media; Ion exchange; Kinetics; Proteins

Indexed keywords

ACETATE BUFFERS; CATION EXCHANGERS; CHARGED POLYMERS; COUNTER-ION EFFECTS; COUNTERIONS; FLEXIBLE NETWORKS; GRAFTED MEDIA; GRAFTED POLYMERS; HOPPING PROCESS; LACTOFERRIN; MACROPOROUS; MASS-TRANSFER KINETICS; MODEL PROTEINS; MONOCLONAL ANTIBODIES (MAB); POLYMER-GRAFTED; PROTEIN ADSORPTION; PROTEIN ADSORPTION KINETICS; PROTEIN BINDING; PROTEIN BINDING STRENGTH; PROTEIN CONCENTRATIONS; PROTEIN DIFFUSION; PROTEIN ION-EXCHANGE; PROTEIN MASS TRANSFER KINETICS; PROTEIN TRANSPORT; STRUCTURE OF THE POLYMERS; UNDERLYING MECHANISM;

AMINO ACIDS; BIOCHEMISTRY; ENZYME KINETICS; GRAFTING (CHEMICAL); ION EXCHANGE; ION EXCHANGERS; IONS; KINETICS; MASS TRANSFER; MONOCLONAL ANTIBODIES; PHASE EQUILIBRIA; PROTEINS; SODIUM; STOICHIOMETRY;

POLYMERS;

AGAROSE; ARGININE; CALCIUM; COUNTERION; LACTOFERRIN; LYSOZYME; MONOCLONAL ANTIBODY; POLYMER; SODIUM; TETRABUTYLAMMONIUM;

ADSORPTION KINETICS; ARTICLE; BINDING AFFINITY; CATION EXCHANGE; CHEMICAL STRUCTURE; DIFFUSION; PRIORITY JOURNAL; PROTEIN BINDING; STOICHIOMETRY;

ADSORPTION; ANIMALS; ANTIBODIES, MONOCLONAL; CATION EXCHANGE RESINS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; KINETICS; LIGHT; MODELS, CHEMICAL; POLYMERS; SCATTERING, RADIATION;

TETRA;

EID: 84864540223     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2012.06.100     Document Type: Article

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