Production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expressing bacterial PNGase F

Plant Biotechnology Journal

Volumn 10, Issue 7, 2012, Pages 773-782

  Mamedov, Tarlan ^a   Ghosh, Ananya ^a   Jones, R Mark ^a   Mett, Vadim ^a   Farrance, Christine E ^a   Musiychuk, Konstantin ^a   Horsey, April ^a   Yusibov, Vidadi ^a  

^a Fraunhofer USA Inc   (United States)

Author keywords

Deglycosylation; Malaria vaccine candidate Pfs48 45; N linked glycosylation; Plant transient expression; PNGase F; Recombinant proteins

Indexed keywords

ANTHRAX TOXIN; BACTERIAL ANTIGEN; BACTERIAL TOXIN; GLYCOPEPTIDASE; MONOCLONAL ANTIBODY; POLYSACCHARIDE; PROTOZOAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIUM; BIOSYNTHESIS; BIOTECHNOLOGY; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYMOLOGY; GLYCOSYLATION; IMMUNOLOGY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PEPTIDE MAPPING; PLASMODIUM FALCIPARUM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; SOLUBILITY; TOBACCO; TRANSGENIC PLANT; WESTERN BLOTTING;

ANTIBODIES, MONOCLONAL; ANTIGENS, BACTERIAL; BACTERIA; BACTERIAL TOXINS; BIOTECHNOLOGY; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLYCOSYLATION; MASS SPECTROMETRY; PEPTIDE MAPPING; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; PLANTS, GENETICALLY MODIFIED; PLASMODIUM FALCIPARUM; POLYSACCHARIDES; PROTEIN BINDING; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY; TOBACCO;

BACTERIA (MICROORGANISMS); EUKARYOTA; MAMMALIA; NICOTIANA BENTHAMIANA; PLASMODIUM (APICOMPLEXA); PLASMODIUM FALCIPARUM; PLASMODIUM PARASITES;

EID: 84864506613     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2012.00694.x     Document Type: Article

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