Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator

Bioresource Technology

Volumn 121, Issue , 2012, Pages 320-327

  Wong, Yoke Ming ^a   Brigham, Christopher J ^b   Rha, ChoKyun ^b   Sinskey, Anthony J ^b   Sudesh, Kumar ^a  

^a SCHOOL OF PHYSICS   (Malaysia)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Bioplastics; Crude palm kernel oil; Cupriavidus necator; Poly(3 hydroxybutyrate co 3 hydroxyhexanoate); Polymer characterization

Indexed keywords

BIOPLASTICS; CUPRIAVIDUS NECATOR; PALM KERNEL OIL; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE); POLYMER CHARACTERIZATION;

BIOCHEMISTRY; CHARACTERIZATION; COPOLYMERS; MECHANICAL PROPERTIES; VEGETABLE OILS;

MONOMERS;

3 HYDROXYHEXANOATE; COPOLYMER; MONOMER; PALM OIL; POLYHYDROXYALKANOIC ACID; UNCLASSIFIED DRUG;

BACTERIUM; BIOCHEMICAL COMPOSITION; CRUDE OIL; MONOCOTYLEDON; NUCLEAR MAGNETIC RESONANCE; POLYMER; RECOMBINATION;

ARTICLE; BIOSYNTHESIS; CARBON NUCLEAR MAGNETIC RESONANCE; CUPRIAVIDUS NECATOR; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE;

BIOTECHNOLOGY; CAPROATES; CHROMATOGRAPHY, GAS; CUPRIAVIDUS NECATOR; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, BIOLOGICAL; PLANT OILS; POLYHYDROXYALKANOATES; TIME FACTORS;

CUPRIAVIDUS NECATOR;

EID: 84864402671     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2012.07.015     Document Type: Article

References (35)

1. Akiyama M., Tsuge T., Doi Y. Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation. Polym. Degrad. Stab. 2003, 80:183-194.
2. Bhubalan K., Rathi D.-N., Abe H., Iwata T., Sudesh K. Improved synthesis of P(3HB-co-3HV-co-3HHx) terpolymers by mutant Cupriavidus necator using the PHA synthase gene of Chromobacterium sp. USM2 with high affinity towards 3HV. Polym. Degrad. Stab. 2011, 95:1436-1442.
3. Braunegg G., Sonnleitner B., Lafferty R.M. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Appl. Microbiol. Biotechnol. 1978, 6:29-37.
4. Buckland B.C., Drew S.W., Connors N.C., Chartrain M.M., Lee C., Salmon P.M., Gbewonyo K., Zhou W., Gailliot P., Singhvi R., Olewinski Jr R.C., Sun W.-J., Reddy J., Zhang J., Jackey B.A., Taylor C., Goklen K.E., Junker B., Greasham R.L. Microbial conversion of indene to indandiol: a key intermediate in the synthesis of CRIXIVAN. Metab. Eng. 1999, 1:63-74.
5. Budde C.F., Riedel S.L., Willis L.B., Rha C., Sinskey A.J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl. Environ. Microbiol. 2011, 77:2847-2854.
6. de Roo G., Ren Q., Witholt B., Kessler B. Development of an improved in vitro activity assay for medium chain length PHA polymerases based on CoenzymeA release measurements. J. Microbiol. Methods 2000, 41:1-8.
7. Doi Y. Microbial polyesters 1990, VCH, New York.
8. Doi Y., Kitamura S., Abe H. Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 1995, 28:4822-4828.
9. Fukui T., Kichise T., Iwata T., Doi Y. Characterization of 13kDa granule-associated protein in Aeromonas caviae and biosynthesis of polyhydroxyalkanoates with altered molar composition by recombinant bacteria. Biomacromolecules 2001, 2:148-153.
10. Gerngross T.U., Martin D.P. Enzyme-catalyzed synthesis of poly[(R)-(-)-3-hydroxybutyrate]: formation of macroscopic granules in vitro. Proc. Natl. Acad. Sci. USA 1995, 92:6279-6283.
11. Gerngross T.U., Snell K.D., Peoples O.P., Sinskey A.J., Csuhai E., Masamune S., Stubbe J. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry 1994, 33:9311-9320.
12. Han J., Qiu Y.-Z., Liu D.-C., Chen G.-Q. Engineered Aeromonas hydrophila for enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with alterable monomers composition. FEMS Microbiol. Lett. 2004, 239:195-201.
13. Hori K., Kobayashi A., Ikeda H., Unno H. Rhodococcus aetherivorans IAR1, a new bacterial strain synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from toluene. J. Biosci. Bioeng. 2009, 107:145-150.
14. Iwata T. Strong fibers and films of microbial polyesters. Macromol. Biosci. 2005, 5:689-701.
15. Jian J., Li Z.J., Ye H.M., Yuan M.Q., Chen G.Q. Metabolic engineering for microbial production of polyhydroxyalkanoates consisting of high 3-hydroxyhexanoate content by recombinant Aeromonas hydrophila. Bioresour. Technol. 2010, 101:6096-6102.
16. Kahar P., Tsuge T., Taguchi K., Doi Y. High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain. Polym. Degrad. Stab. 2004, 83:79-86.
17. Kichise T., Fukui T., Yoshida Y., Doi Y. Biosynthesis of polyhydroxyalkanoates (PHA) by recombinant Ralstonia eutropha and effects of PHA synthase activity on in vivo PHA biosynthesis. Int. J. Biol. Macromol. 1999, 25:69-77.
18. Madison L.L., Huisman G.W. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 1999, 63:21-53.
19. Mifune J., Nakamura S., Fukui T. Targeted engineering of Cupriavidus necator chromosome for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Can. J. Chem. 2008, 86:621-627.
20. Mifune J., Nakamura S., Fukui T. Engineering of pha operon on Cupriavidus necator chromosome for efficient biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Polym. Degrad. Stab. 2010, 95:1305-1312.
21. MPOB, Malaysian Palm Oil Board, 2010. Overview of the Malaysian oil palm industry 2010. Available online at [last Accessed December, 2011]. http://econ.mpob.gov.my/economy/Overview_2010_final.pdf.
22. Ng K.-S., Wong Y.-M., Tsuge T., Sudesh K. Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymers using jatropha oil as the main carbon source. Process Biochem. 2011, 46:1572-1578.
23. Reddy, C.S.K., Ghai, R., Rashmi, and Kalia, V.C., 2003. Polyhydroxyalkanoates: an overview. Bioresour. Technol. 87, 137-146.
24. Rehm B.H.A. Polyester synthases: natural catalysts for plastics. Biochem. J. 2003, 37:15-33.
25. Riedel S.L., Bader J., Brigham C.J., Budde C.F., Yusof Z.A.M., Rha C.K., Sinskey A.J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations. Biotechnol. Bioeng. 2012, 109:74-83.
26. Schubert P., Steinbuchel A., Schlegel H.G. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. J. Bacteriol. 1988, 170:5837-5847.
27. Shimamura E., Kasuya K., Kobayashi G., Shiotani T., Shima Y., Doi Y. Physical properties and biodegradability of microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 1994, 27:878-880.
28. Sim S.J., Snell K.D., Hogan S.A., Stubbe J., Rha C., Sinskey A.J. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo. Nat. Biotechnol. 1997, 15:63-67.
29. Sudesh K., Abe H., Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog. Polym. Sci. 2000, 25:1503-1555.
30. Takase K., Matsumoto K., Taguchi S., Doi Y. Alteration of substrate chain-length specificity of type II synthase for polyhydroxyalkanoate biosynthesis by in vitro evolution: in vivo and in vitro enzyme assays. Biomacromolecules 2004, 5:480-485.
31. Valentin H.E., Steinbüchel A. Application of enzymatically synthesized short-chain-length hydroxy fatty acid coenzyme A thioesters for assay of polyhydroxyalkanoic acid synthases. Appl. Microbiol. Biotechnol. 1994, 40:699-709.
32. van der Walle G.A.M., Buisman G.J.H., Weusthuis R.A., Eggink G. Development of environmentally friendly coatings and paints using medium-chain-length poly(3-hydroxyalkanoates) as the polymer binder. Int. J. Biol. Macromol. 1999, 25:123-128.
33. Wang H.H., Zhou X.R., Liu Q., Chen G.Q. Biosynthesis of polyhydroxyalkanoate homopolymers by Pseudomonas putida. Appl. Microbiol. Biotechnol. 2011, 89:1497-1507.
34. Ward P.G., de Roo G., O'Connor K.E. Accumulation of polyhydroxyalkanoate from styrene and phenylacetic acid by Pseudomonas putida CA-3. Appl. Environ. Microbiol. 2005, 71:2046-2052.
35. Watanabe T., He Y., Fukuchi T., Inoue Y. Comonomer compositional distribution and thermal characteristics of bacterially synthesized poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)s. Macromol. Biosci. 2001, 1:75-83.