Rapid detection and identification of human hookworm infections through high resolution melting (HRM) analysis

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Ngui, Romano ^a   Lim, Yvonne A L ^a   Chua, Kek Heng ^a  

^a UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

INTERNAL TRANSCRIBED SPACER 2;

AMPLICON; ANCYLOSTOMA; ANCYLOSTOMA BRAZILIENSE; ANCYLOSTOMA CANINUM; ANCYLOSTOMA CEYLANICUM; ANCYLOSTOMA DUODENALE; ARTICLE; CONTROLLED STUDY; GENETIC MARKER; HIGH RESOLUTION MELTING ANALYSIS; HOOKWORM INFECTION; INTERMETHOD COMPARISON; NECATOR AMERICANUS; NONHUMAN; PARASITE IDENTIFICATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES IDENTIFICATION;

ANCYLOSTOMA; ANIMALS; DNA PRIMERS; DNA, INTERGENIC; DNA, RIBOSOMAL; GENETIC MARKERS; HOOKWORM INFECTIONS; HUMANS; NECATOR; NUCLEIC ACID DENATURATION; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

ANCYLOSTOMA; ANCYLOSTOMA CANINUM; ANCYLOSTOMA CEYLANICUM; ANCYLOSTOMA DUODENALE; ANCYLOSTOMATOIDEA; NECATOR AMERICANUS;

EID: 84864390277     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0041996     Document Type: Article

References (41)

1. Hotez PJ, (2009) One World Health: Neglected Tropical Diseases in a Flat World. PLoS Negl Trop Dis 3 (4): e405.
2. Ngui R, Lim YAL, Traub R, Mahmud R, Mistam MS, (2012) Epidemiological and genetic data supporting the transmission of Ancylostoma ceylanicum among human and domestic animals. PLoS Negl Trop Dis 6 (2): e1522.
3. Jiraanankul V, Aphijirawat W, Mungthin M, Khositnithikul R, Rangsin R, et al. (2011) Incidence and risk factors of hookworm infection in a rural community of central Thailand. Am J Trop Med Hyg 84: 594-598.
4. Sato M, Sanguankiat S, Yoonuan T, Pongvongsa T, Keomoungkhoun M, et al. (2010) Copro-molecular identification of infections with hookworm eggs in rural Lao PDR. Trans R Soc Trop Med Hyg 104: 617-622.
5. de Silva NR, Brooker S, Hotez PJ, Montresor A, Engels D, et al. (2003) Soil-transmitted helminth infections: Updating the global picture. Trends Parasitol 19: 547-551.
6. Stoltzfus RJ, Chwaya HM, Tielsch JM, Schulze KJ, Albonico M, et al. (1997) Epidemiology of iron deficiency anemia in Zanzibari schoolchildren: the importance of hookworms. Am J Clin Nutr 65: 153-159.
7. Albonico M, Crompton DW, Savioli L, (1999) Control strategies for human intestinal nematode infections. Adv Parasitol 42: 277-341.
8. Crompton DWT, (2000) The public health importance of hookworm disease. Parasitology 121: 39-50.
9. Brooker S, Bethony J, Hotez PJ, (2008) Human Hookworm Infection in the 21st Century. Adv Parasitol 58: 197-288.
10. Gasser RB, (2006) Molecular tools-advances, opportunities and prospects. Vet Parasitol 136: 69-89.
11. Gasser RB, Chilton NB, Hoste H, Beveridge I, (1993) Rapid sequencing of rDNA from single worms and eggs of parasitic helminthes. Nucleic Acids Res 21: 2525-2526.
12. Gasser RB, Monti JR, (1997) Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA. Mol Cell Probes 1: 201-209.
13. Gasser RB, Monti JR, Bao-Zhen Q, Polderman AM, Nansen P, et al. (1998) A mutation scanning approach for the identification of hookworm species and analysis of population variation. Mol Biochem Parasitol 92: 303-312.
14. Traub RJ, Robertson ID, Irwin P, Mencke N, Thompson RC, (2004) Application of a species-specific PCR-RFLP to identify Ancylostoma eggs directly from canine feces. Vet Parasitol 123: 245-255.
15. Verweij JJ, Brienen EA, Ziem J, Yelifari L, Polderman AM, et al. (2007) Simultaneous detection and quantification of Ancylostoma duodenale, Necator americanus and Oesophagostomum bifurcum in fecal samples using multiplex real-time PCR. Am J Trop Med Hyg 77: 685-690.
16. Basuni M, Muhi J, Othman N, Verweij JJ, Ahmad M, et al. (2011) A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths. Am J Trop Med Hyg 84: 338-343.
17. Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ, (2003) High-resolution genotyping by amplicon melting analysis using LC Green. Clin Chem 49: 853-860.
18. Liew M, Pryor R, Palais R, Meadows C, Erali M, et al. (2004) Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem 50: 1156-1164.
19. Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, et al. (2004) High-resolution DNA melting curve analysis to establish HLA genotypic identity. Tissue Antigens 64: 156-164.
20. Radvansky J, Resko P, Surovy M, Minarik G, Ficek A, et al. (2010) High-resolution melting analysis for genotyping of the myotonic dystrophy type1 associated Alu insertion/deletion polymorphism. Anal Biochem 398: 126-128.
21. Saitsu H, Kato M, Okada I, Orii KE, Higuchi T, et al. (2010) STXBP1 mutations in early infantile epileptic encephalopathy with suppression-burst pattern. Epilepsia 51: 2397-23405.
22. Talmi-Frank D, Nasereddin A, Schnur LF, Schonian G, Toz SO, et al. (2010) Detection and identification of Old World Leishmania by high resolution melt analysis. PLoS Negl Trop Dis 4 (1): e581.
23. Pangasa A, Jex AR, Campbell BE, Bott NJ, Whipp M, et al. (2009) High resolution melting-curve (HRM) analysis for the diagnosis of cryptosporidiosis in humans. Mol Cell Probes 23: 10-15.
24. Andriantsoanirina V, Lascombes V, Ratsimbasoa A, Bouchier C, Hoffman J, et al. (2009) Rapid detection of point mutations in Plasmodium falciparum genes associated with antimalarial drugs resistance by using high resolution melting analysis. J Microbiol Meth 78: 165-170.
25. Hussein EM, Al-Mohammed HI, Hussein AM, (2009) Genetic diversity of Dientamoeba fragilis isolates of irritable bowel syndrome patients by high-resolution melting-curve (HRM) analysis. Parasitol Res 105: 1053-1060.
26. Robinson BS, Monis PT, Dobson PJ, (2006) Rapid, sensitive and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis. Appl Environ Microbiol 72: 5857-5863.
27. Bienz M, Siles-Lucas M, Muller N, (2001) Use of novel DNA melting profile assay for the identification of PCR-amplified genomic sequences encoding for variant specific surface proteins from the clonal GS/M-83-H7 line of Giardia lamblia. Parasitol Res 87: 1011-1015.
28. Areekit S, Kanjanavas P, Pakpitchareon A, Khawsak P, Khuchareontaworn S, et al. (2009) High resolution melting real-time PCR for rapid discrimination between Brugia malayi and Brugia pahangi. J Med Assoc Thai 92: S24-28.
29. Radvansky J, Bazsalovicsova E, Kralova-Hromadova I, Minarik G, Kadasi L, (2011) Development of high-resolution melting (HRM) analysis for population studies of Fascioloides magna (Trematoda: Fasciolidae), the giant liver fluke of ruminants. Parasitol Res 108: 201-209.
30. Hall TA, (1999) BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Series 41: 95-98.
31. Teh CSJ, Chua KH, Thong KL, (2010) Simultaneous differential detection of human pathogenic and non-pathogenic Vibrio species using a multiplex PCR based on gyrB and pntA genes. J Appl Microbiol 108: 1940-1945.
32. Thong KL, Lai MY, Teh CSJ, Chua KH, (2011) Simultaneous detection of Methicillin-resistant Staphylococcus aureus, Acinetobacter baumanii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa by multiplex PCR. Trop Biomed 28: 21-31.
33. Ng ZX, Kuppusamy UR, Tajunisah I, Fong KCS, Koay ACA, et al. (2012) 2245G/A polymorphism of the receptor for advanced glycation end-products (RAGE) gene is associated with diabetic retinopathy in the Malaysian population. BJ Ophthalmol 96: 289-292.
34. Ngui R, Lee SC, Tan TK, Muhammad Aidil R, Lim YAL, (2012) Molecular identification of human hookworm infections in economic disadvantaged communities of Peninsular Malaysia. Am J Trop Med Hyg 86: 837-842.
35. Reed GH, Wittwer CT, (2004) Sensitivity and specificity of single nucleotide polymorphism scanning by high-resolution melting analysis. Clin Chem 50: 1748-1754.
36. Montgomery J, Wittwer CT, Palais R, Zhou L, (2007) Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis. Nat Protoc 2: 59-66.
37. Monis PT, Giglio S, Saint PC, (2005) Comparison of SYTO9 and SYBR Green I for real time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Anal Biochem 340: 24-34.
38. Rasmussen JP, Saint CP, Monis PT, (2007) Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes. BMC Bioinformatics 8: 107.
39. de Gruijter JM, van Lieshout L, Gasser RB, Verweij JJ, Brienen EA, et al. (2005) Polymerase chain reaction-based differential diagnosis of Ancylostoma duodenale and Necator americanus infections in human in northern Ghana. Trop Med Int Health 10: 574-580.
40. Stanley KK, Szewczuk E, (2005) Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res 33: e180.
41. Poulson MD, Wittwer CT, (2007) Closed-tube genotyping of apolipoprotein E by isolated-probe PCR with multiple unlabeled probes and high-resolution DNA melting analysis. Biotechniques 43: 87-91.