Rapid detection of point mutations in Plasmodium falciparum genes associated with antimalarial drugs resistance by using High-Resolution Melting analysis

Journal of Microbiological Methods

Volumn 78, Issue 2, 2009, Pages 165-170

  Andriantsoanirina, Valérie ^a   Lascombes, Vincent ^a   Ratsimbasoa, Arsène ^a   Bouchier, Christiane ^b   Hoffman, Jonathan ^a   Tichit, Magali ^b   Rabarijaona, Leon Paul ^a   Durand, Rémy ^c   Ménard, Didier ^a  

^a INSTITUT PASTEUR DE MADAGASCAR   (Madagascar)

^b INSTITUT PASTEUR   (France)

^c AVICENNE HOSPITAL   (France)

Author keywords

Antimalarial drug resistance; Genotyping; HRM; Malaria; Plasmodium falciparum; Real time PCR; SNP

Indexed keywords

ANTIMALARIAL AGENT;

ACCURACY; ANTIMALARIAL DRUG RESISTANCE; ARTICLE; CONTROLLED STUDY; DNA DENATURATION; DNA SEQUENCE; FLUORESCENCE ANALYSIS; GENE IDENTIFICATION; GENETIC MARKER; MELTING POINT; NONHUMAN; PARASITE ISOLATION; PLASMODIUM FALCIPARUM; POINT MUTATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

ANIMALS; ANTIMALARIALS; DNA, PROTOZOAN; DRUG RESISTANCE; HOT TEMPERATURE; HUMANS; NUCLEIC ACID DENATURATION; PARASITIC SENSITIVITY TESTS; PLASMODIUM FALCIPARUM; POINT MUTATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRANSITION TEMPERATURE;

PLASMODIUM FALCIPARUM;

EID: 67650154172     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.05.013     Document Type: Article

References (30)

1. Bass C., Nikou D., Donnelly M.J., Williamson M.S., Ranson H., Ball A., Vontas J., and Field L.M. Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods. Malar. J. 6 (2007) 111
2. Barnadas C., Ratsimbasoa A., Tichit M., Bouchier C., Jahevitra M., Picot S., and Ménard D. Plasmodium vivax resistance to chloroquine in Madagascar: clinical efficacy and polymorphisms in pvmdr1 and pvcrt-o genes. Antimicrob. Agents Chemother. 52 (2008) 4233-4240
3. Barnadas C., Tichit M., Bouchier C., Ratsimbasoa A., Randrianasolo L., Raherinjafy R., Jahevitra M., Picot S., and Ménard D. Plasmodium vivax dhfr and dhps mutations in isolates from Madagascar and therapeutic response to sulphadoxine-pyrimethamine. Malar. J. 7 (2008) 35
4. Chateigner-Boutin A.L., and Small I. A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons. Nucleic Acids Res. 35 (2007) e114
5. Durand R., Eslahpazire J., Jafari S., Delabre J.F., Marmorat-Khuong A., Di Piazza J.P., and Le Bras J. Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum. Antimicrob. Agents Chemother. 44 (2000) 3461-3464
6. Durand R., Huart V., Jafari S., and Le Bras J. Rapid detection of a molecular marker for chloroquine-resistant falciparum malaria. Antimicrob. Agents Chemother. 46 (2002) 2684-2686
7. Herrmann M.G., Durtschi J.D., Bromley L.K., Wittwer C.T., and Voelkerding K.V. Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes. Clin. Chem. 52 (2006) 494-503
8. Kristensen L.S., and Dobrovic A. Direct genotyping of single nucleotide polymorphisms in methyl metabolism genes using probe-free high-resolution melting analysis. Cancer Epidemiol. Biomark. Prev. 17 (2008) 1240-1247
9. Le Bras J., and Durand R. The mechanisms of resistance to antimalarial drugs in Plasmodium falciparum. Fund. Clin. Pharmacol. 17 (2003) 147-153
10. Lee L.G., Connell C.R., and Bloch W. Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Res. 21 (1993) 3761-3766
11. Liew M., Pryor R., Palais R., Meadows C., Erali M., Lyon E., and Wittwer C. Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin. Chem. 50 (2004) 1156-1164
12. Livak K.J., Flood S.J., Marmaro J., Giusti W., and Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 4 (1995) 357-362
13. Mangold K.A., Manson R.U., Koay E.S., Stephens L., Regner M., Thomson R.B., Peterson L.R., and Kaul K.L. Real-time PCR for detection and identification of Plasmodium spp. J. Clin. Microbiol. 43 (2005) 2435-2440
14. Ménard D., Ratsimbasoa A., Randrianarivelojosia M., Rabarijaona L.P., Raharimalala L., Domarle O., Randrianasolo L., Randriamanantena A., Jahevitra M., Andriantsoanirina V., Rason M.A., Raherinjafy R., Rakotomalala E., Tuseo L., and Raveloson A. Assessment of the efficacy of antimalarial drugs recommended by the National Malaria Control Programme in Madagascar: up-dated baseline data from randomized and multi-site clinical trials. Malar. J. 7 (2008) 55
15. Nair S., Brockman A., Paiphunb L., Nosten F., and Anderson T.J.C. Rapid genotyping of loci involved in antifolate drug resistance in Plasmodium falciparum by primer extension. Int. J. Parasitol. 32 (2002) 852-858
16. Olliaro P. Drug resistance hampers our capacity to roll back malaria. Clin. Infect. Dis. 41 (2005) S247-S257
17. Pangasa A., Jex A.R., Campbell B.E., Bott N.J., Whipp M., Hogg G., Stevens M.A., and Gasser R.B. High resolution melting-curve (HRM) analysis for the diagnosis of cryptosporidiosis in humans. Mol. Cell. Probes 23 (2009) 10-15
18. Plowe C.V., Kublin J.G., and Doumbo O.K. Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase mutations: epidemiology and role in clinical resistance to antifolates. Drug Resist. Updat. 1 (1998) 389-396
19. Rakotonirina H., Barnadas C., Raherijafy R., Andrianantenaina H., Ratsimbasoa A., Randrianasolo L., Jahevitra M., Andriantsoanirina V., and Ménard D. Accuracy and reliability of malaria diagnostic techniques for guiding febrile outpatient treatment in malaria-endemic countries. Am. J. Trop. Med. Hyg. 78 (2008) 217-221
20. Reed G.H., and Wittwer C.T. Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clin. Chem. 50 (2004) 1748-1754
21. Reed M.B., Saliba K.J., Caruana S.R., Kirk K., and Cowman A.F. Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum. Nature 403 (2000) 906-909
22. Saiki R.K., Bugawan T.L., Horn G.T., Mullis K.B., and Erlich H.A. Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature 324 (1986) 163-166
23. Snow R.W., Guerra C.A., Noor A.M., Myint H.Y., and Hay S.I. The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature 434 (2005) 214-217
24. Stephens A.J., Inman-Bamber J., Giffard P.M., and Huygens F. High-resolution analysis of the spa repeats region of Staphylococcus aureus. Clin. Chem. 54 (2008) 432-436
25. Tajiri-Utagawa E., Hara M., Takahashi K., Watanabe M., and Wakita T. Development of a rapid high-throughput method in high-resolution melting (HRM) analysis for routine detection and genotyping of noroviruses. J. Clin. Microbiol. 47 (2009) 435-440
26. Wellems T.E., and Plowe C.V. Chloroquine-resistant malaria. J. Infect. Dis. 184 (2001) 770-776
27. Wilson P.E., Alker A.P., and Meshnick S.R. Real-time PCR methods for monitoring antimalarial drug resistance. Trends Parasitol. 21 (2005) 278-283
28. Zhou X.H., Obuchowski N.A., and McClish D.K. Statistical Methods in Diagnostic Medicine (2002), Wiley, New York
29. Zhou L., Wang L., Palais R., Pryor R., and Wittwer C.T. High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution. Clin. Chem. 51 (2005) 1770-1777
30. Zhou Z., Poe A.C., Limor J., Grady K.K., Goldman I., McCollum A.M., Escalante A.A., Barnwell J.W., and Udhayakumar V. Pyrosequencing, a high-throughput method for detecting single polymorphism in the dihydrofolate reductase and dihydropteroate synthetase genes of Plasmodium falciparum. J.Clin. Microbiol. 44 (2006) 3900-3910