Use of a novel DNA melting profile assay for the identification of PCR-amplified genomic sequences encoding for variant-specific surface proteins from the clonal GS/M-83-H7 line of Giardia lamblia

Parasitology Research

Volumn 87, Issue 12, 2001, Pages 1011-1015

  Bienz, Marianne ^a   Siles Lucas, Mar ^a   Müller, Norbert ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MEMBRANE ANTIGEN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG; VARIANT SPECIFIC SURFACE ANTIGEN H7; VARIANT SPECIFIC SURFACE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ASSAY; CONTROLLED STUDY; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE SEQUENCE; GENOTYPE; GIARDIA LAMBLIA; MOLECULAR CLONING; MOLECULE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; TEMPERATURE; TROPHOZOITE;

GIARDIA INTESTINALIS; PROTOZOA;

EID: 0034756618     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004360100490     Document Type: Article

References (12)

1. Cysteine-rich variant surface proteins of Giardia lamblia
2. Vsp gene expression by Giardia lamblia clone GS/M-83-H7 during antigenic variation in vivo and in vitro
3. Amino acid substitutions matrices from protein blocks
4. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile
5. PCR/SSCP detects reliably and efficiently DNA sequence variations in large scale screening projects
6. Antigenic variation and the murine immune response to Giardia lamblia
7. Surface antigen variability and variation in Giardia lamblia
8. Giardia lamblia: Identification and characterization of a variant-specific surface protein gene family
9. Product differentiation by analysis of DNA melting curve the polymerase chain reaction
10. Amino acid substitutions in structurally related proteins. A pattern recognition approach. Determination of a new and efficient scoring matrix
11. Continuous fluorescence monitoring of rapid cycle DNA amplification