ExoU activates NF-κB and increases IL-8/KC secretion during pseudomonas aeruginosa infection

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  de Lima, Carolina Diettrich Mallet ^a   Calegari Silva, Teresa Cristina ^b   Pereira, Renata Meirelles Santos ^b   Santos, Sabrina Alves de Oliveira Lima ^a   Lopes, Ulisses Gazos ^b   Plotkowski, Maria Cristina Maciel ^a   Saliba, Alessandra Mattos ^a  

^a STATE UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; EXOU PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 8; MESSENGER RNA; PHOSPHOLIPASE A2; TRANSCRIPTION FACTOR RELA; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BINDING AFFINITY; BINDING SITE; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; CYTOKINE RELEASE; FEMALE; GENE; GENE EXPRESSION; HUMAN; HUMAN CELL; INTERLEUKIN 8 GENE; MOUSE; NEUTROPHIL CHEMOTAXIS; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN TRANSPORT; PSEUDOMONAS INFECTION; TYPE III SECRETION SYSTEM;

ANIMALS; BACTERIAL PROTEINS; BRONCHOALVEOLAR LAVAGE FLUID; CAPILLARIES; CELL LINE; ENDOTHELIAL CELLS; EPITHELIAL CELLS; FEMALE; GENE EXPRESSION REGULATION; INTERLEUKIN-8; MICE; NEUTROPHIL INFILTRATION; NF-KAPPA B; PSEUDOMONAS AERUGINOSA; RESPIRATORY SYSTEM; RNA, MESSENGER;

MURINAE; MUS; PSEUDOMONAS AERUGINOSA;

EID: 84864387232     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0041772     Document Type: Article

References (34)

1. Schulert GS, Feltman H, Rabin SDP, Martin CG, Battle SE, et al. (2003) Secretion of the toxin ExoU is a marker for highly virulent Pseudomonas aeruginosa isolates obtained from patients with hospital-acquired pneumonia. J Infect Dis 188: 1695-1706.
2. Wareham DW, Curtis MA, (2007) A genotypic and phenotypic comparison of type III secretion profiles of Pseudomonas aeruginosa cystic fibrosis and bacteremia isolates. Int J Med Microbiol 297: 227-234.
3. Engel J, Balachandran P, (2009) Role of Pseudomonas aeruginosa type III effectors in disease. Curr Opin Microbiol 12: 61-66.
4. Saliba AM, Nascimento DO, Silva MCA, Assis MC, Gayer CRM, et al. (2005) Eicosanoid-mediated proinflammatory activity of Pseudomonas aeruginosa ExoU. Cell.Microbiol 7: 1811-1822.
5. Hoffmann E, Dittrich-Breiholz O, Holtmann H, Kracht M, (2002) Multiple control of interleukin-8 gene expression. J Leukoc Biol 72: 847-855.
6. Rabin SDP, Hauser AR, (2005) Functional regions of the Pseudomonas aeruginosa cytotoxin ExoU. Infect Immun 73: 573-582.
7. Wu W, Booth JL, Coggeshall KM, Metcalf JP, (2006) Calcium-dependent viral internalization is required for adenovirus type 7 induction of IL-8 protein. Virology 355: 18-29.
8. Cuzick A, Stirling FR, Lindsay SL, Evans TJ, (2006) The type III pseudomonal Exotoxin U activates the c-Jun NH2-terminal kinase pathway and increases human epithelial Interleukin-8 production. Infect Immun 74: 4104-4113.
9. Diaz MH, Shaver CM, King JD, Musunuri S, Kazzaz JA, et al. (2008) Pseudomonas aeruginosa induces localized immunosuppression during pneumonia. Infect Immun 76: 4414-4421.
10. Koh AY, Priebe GP, Ray C, Van Rooijen N, Pier GB, (2009) Inescapable need for neutrophils as mediators of cellular innate immunity to acute Pseudomonas aeruginosa pneumonia. Infect Immun 77: 5300-5310.
11. Sun L, Guo R-F, Newstead MW, Standiford TJ, Macariola DR, et al. (2009) Effect of IL-10 on neutrophil recruitment and survival after Pseudomonas aeruginosa challenge. Am J Respir Cell Mol Biol 41: 76-84.
12. Williams BJ, Dehnbostel J, Blackwell S, (2010) Pseudomonas aeruginosa: host defence in lung diseases. Respirology 15: 1037-1056.
13. Diaz MH, Hauser AR, (2010) Pseudomonas aeruginosa cytotoxin ExoU is injected into phagocytic cells during acute pneumonia. Infect Immun 78: 1447-1456.
14. Lins RX, Assis MC, Lima CDM, Freitas C, Plotkowski MCM, et al. (2010) ExoU modulates soluble and membrane-bound ICAM-1 in Pseudomonas aeruginosa-infected endothelial cells. Microbes Infect 12: 154-161.
15. Plotkowski MC, Brandão BA, Assis MC, Feliciano LFP, Raymond B, et al. (2008) Lipid body mobilization in the ExoU-induced release of inflammatory mediators by airway epithelial cells. Microb Pathog 45: 30-37.
16. Bezzerri V, Borgatti M, Finotti A, Tamanini A, Gambari R, et al. (2011) Mapping the transcriptional machinery of the IL-8 gene in human bronchial epithelial cells. J Immunol 187: 6069-6081.
17. Arbibe L, Kim DW, Batsche E, Pedron T, Mateescu B, et al. (2007) An injected bacterial effector targets chromatin access for transcription factor NF-κB to alter transcription of host genes involved in immune responses. Nat Immunol 8: 47-56.
18. Schmeck B, Lorenz J, N'Guessan PD, Opitz B, van Laak V, et al. (2008) Histone acetylation and flagellin are essential for Legionella pneumophila-induced cytokine expression. J Immunol 181: 940-947.
19. Raymond B, Batsche E, Boutillon F, Wu YZ, Leduc D, et al. (2009) Anthrax lethal toxin impairs IL-8 expression in epithelial cells through inhibition of histone H3 modification. PLoS Pathog 5: e1000359.
20. Angrisano T, Pero R, Peluso S, Keller S, Sacchetti S, et al. (2010) LPS-hinduced IL-8 activation in human intestinal epithelial cells is accompanied by specific histone H3 acetylation and methylation changes. BMC Microbiol 10: 172.
21. Ghizzoni M, Haisma HJ, Maarsingh H, Dekker FJ, (2011) Histone acetyltransferases are crucial regulators in NF-κB mediated inflammation. Drug Discov Today 16: 504-511.
22. Fu Z, Bettega K, Carroll S, Buchholz KR, Machen TE, (2007) Role of Ca2+ in responses of airway epithelia to Pseudomonas aeruginosa, flagellin, ATP, and thapsigargin. Am J Physiol Lung Cell Mol Physiol 292: L353-L364.
23. Shanks KK, Guang W, Kim KC, Lillehoj EP, (2010) Interleukin-8 production by human airway epithelial cells in response to Pseudomonas aeruginosa clinical isolates expressing type a or type b flagellins. Clin Vaccine Immunol 17: 1196-1202.
24. Saliba AM, Assis MC, Nishi R, Raymond B, Marques EA, et al. (2006) Implications of oxidative stress in the cytotoxicity of Pseudomonas aeruginosa ExoU. Microbes Infect 8: 450-459.
25. Rahman I, Gilmour PS, Jimenez LA, MacNee W, (2002) Oxidative stress and TNF-α induce histone acetylation and NF-κB/AP-1 activation in alveolar epithelial cells: Potential mechanism in gene transcription in lung inflammation. Mol Cell Biochem 234/235: 239-248.
26. Moodie FM, Marwick JA, Anderson CS, Szulakowski P, Biswas SK, et al. (2004) Oxidative stress and cigarette smoke alter chromatin remodeling but differentially regulate NF-κB activation and proinflammatory cytokine release in alveolar epithelial cells. FASEB J 18: 1897-1899.
27. Hou Y, An J, Hu XR, Sun BB, Lin J, et al. (2009) Ghrelin inhibits interleukin-8 production induced by hydrogen peroxide in A549 cells via NF-κB pathway. Int Immunopharmacol 9: 120-126.
28. Ye SF, Wu YH, Hou ZQ, Zhang QQ, (2009) ROS and NF-κB are involved in upregulation of IL-8 in A549 cells exposed to multi-walled carbon nanotubes. Biochem Biophys Res Commun 379: 643-648.
29. Maybin JA, Hirani N, Jabbour HN, Critchley HOD, (2011) Novel roles for hypoxia and prostaglandin E2 in the regulation of IL-8 during endometrial repair. Am J Pathol 178: 1245-1256.
30. Machado GB, Oliveira AV, Saliba AM, Lima CDM, Suassuna JHR, et al. (2011) Pseudomonas aeruginosa toxin ExoU induces a PAF-dependent impairment of alveolar fibrin turnover secondary to enhanced activation of coagulation and increased expression of plasminogen activator inhibitor-1 in the course of mice pneumosepsis. Respir Res 12: 104.
31. Borthakur A, Bhattacharyya S, Alrefai WA, Tobacman JK, Ramaswamy K, et al. (2010) Platelet-Activating Factor-induced NF-κB activation and IL-8 production in intestinal epithelial cells are Bcl10-dependent. Inflamm Bowel Dis 16: 593-603.
32. Zheng C, Yin Q, Wu H, (2011) Structural studies of NF-κB signaling. Cell Res 21: 183-195.
33. Ades EW, Candal FJ, Swerlick RA, Summers GVG, Bosse DC, et al. (1992) HMEC-1: Establishment of an immortalized human microvascular endothelial cell line. J Invest Dermatol 99: 683-690.
34. Scheinman RI, Gualberto A, Jewell CM, Cidlowski JA, Baldwin AS Jr, (1995) Characterization of mechanisms involved in transrepression of NF-κB by activated glucocorticoid receptors. Mol Cell Biol 15: 943-953.