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Volumn 12, Issue 1, 2009, Pages 61-66

Role of Pseudomonas aeruginosa type III effectors in disease

Author keywords

[No Author keywords available]

Indexed keywords

ADP RIBOSE TRANSFERASES; BACTERIAL PROTEINS; BACTERIAL TOXINS; ENZYMES; GLUCOSYLTRANSFERASES; GTPASE-ACTIVATING PROTEINS; HOST-PATHOGEN INTERACTIONS; HUMANS; MEMBRANE TRANSPORT PROTEINS; PROTEIN TRANSPORT; PSEUDOMONAS AERUGINOSA; VIRULENCE FACTORS;

EID: 59849110833     PISSN: 13695274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mib.2008.12.007     Document Type: Review
Times cited : (264)

References (50)
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    • Persistent infection with Pseudomonas aeruginosa in ventilator-associated pneumonia
    • This study demonstrates that type III secretion-producing strains of P. aeruginosa are less likely to be cleared from lungs of mechanically ventilated patients after 8 days of appropriate antibiotics and provides support for the current practice of treating these patients for a longer period of time.
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    • This study, along with [25] demonstrates that the membrane localization domain of ExoS is required for localization of translocated ExoS to the plasma membrane followed appearance in perinuclear regions, where it was found associated with 14-3-3 proteins and Rab 5, Rab 6, and Rab 9. Membrane localization was required for ADP ribosylation of Ras and for GAP activity toward Rac. Together, these results suggest that membrane localization helps to specify target modification.
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    • ••], demonstrates that in addition to the previously known ability of ExoT to inhibit cell migration, each domain of ExoT can independently inhibit cell division. The GAP activity of ExoT inhibits Rho, which is required at early stages of cytokinesis. The ADPRT activity, by inhibiting Crk function, prevents the final stage of cytokinesis, daughter cell separation. These studies provide the first evidence for the involvement of Crk and other focal adhesion proteins in cytokinesis.
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    • The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells
    • This paper, along with [28], reveals unexpected roles for ExoT and for Crk in host cell biology. The authors demonstrate that ExoT is necessary and sufficient to induce apoptosis, probably through inactivation of Crk adaptor proteins. Together with its ability to inhibit cytokinesis and cell migration, this study demonstrates why ExoT is a potent inhibitor of wound repair.
    • Shafikhani S.H., Morales C., and Engel J. The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells. Cell Microbiol 10 (2008) 994-1007. This paper, along with [28], reveals unexpected roles for ExoT and for Crk in host cell biology. The authors demonstrate that ExoT is necessary and sufficient to induce apoptosis, probably through inactivation of Crk adaptor proteins. Together with its ability to inhibit cytokinesis and cell migration, this study demonstrates why ExoT is a potent inhibitor of wound repair.
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    • The ubiquitin ligase Cbl-b limits Pseudomonas aeruginosa exotoxin T-mediated virulence
    • Through in vitro and in vivo studies, the authors demonstrate that by binding Crk, ExoT recruits the ubiquitin ligase Cbl-b. ExoT becomes ubiquinated and undergoes proteosomal degradation. Enhanced bacterial persistence and dissemination are observed in a Cbl-b deficient mouse in an ExoT-specific manner. This study represents the first identification of a mammalian gene product that is specifically required for in vivo resistance to disease mediated by a T3SS effector.
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    • Critical role for Ipaf in Pseudomonas aeruginosa-induced caspase-1 activation
    • The authors show that P. aeruginosa flagellin was essential for caspase-1-dependent activation of IL-1beta and that neither ExoS, ExoT, nor ExoY inhibited this Ipaf-dependent pathway.
    • Franchi L., Stoolman J., Kanneganti T.D., Verma A., Ramphal R., and Nunez G. Critical role for Ipaf in Pseudomonas aeruginosa-induced caspase-1 activation. Eur J Immunol 37 (2007) 3030-3039. The authors show that P. aeruginosa flagellin was essential for caspase-1-dependent activation of IL-1beta and that neither ExoS, ExoT, nor ExoY inhibited this Ipaf-dependent pathway.
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    • •], the authors had shown that ExoS inhibits caspase-1-dependent activation of IL-1beta by the P. aeruginosa T3SS.
    • •], the authors had shown that ExoS inhibits caspase-1-dependent activation of IL-1beta by the P. aeruginosa T3SS.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.