Lentivirus-delivered ZEB-1 small interfering RNA inhibits lung adenocarcinoma cell growth in vitro and in vivo

Journal of Cancer Research and Clinical Oncology

Volumn 138, Issue 8, 2012, Pages 1329-1338

  Liu, Yi ^a   Yan, Xiaolong ^a   Liu, Na ^a   Zhou, Jing ^a   Liu, Jiayu ^a   Pang, Hailin ^a   Cao, Jian ^c   Liu, Yanfang ^b   Wang, Yanxia ^b   Liu, Lili ^a   Zhang, Helong ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^c Third Hospital of PLA   (China)

Author keywords

Apoptosis; Cell cycle; Lentivirus; Lung adenocarcinoma; Proliferation; ZEB1

Indexed keywords

HOMEODOMAIN PROTEIN; LENTIVIRUS VECTOR; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; ZINC FINGER E BOX BINDING HOMEOBOX 1 PROTEIN;

ADULT; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CARCINOGENICITY; CELL CYCLE; CELL CYCLE S PHASE; CELL PROLIFERATION; CONTROLLED STUDY; DRUG EFFECT; FEMALE; GENE; GENE EXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; IN VIVO STUDY; LUNG ADENOCARCINOMA; LUNG FIBROBLAST; MAJOR CLINICAL STUDY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; TUMOR GROWTH; ZEB1 GENE;

ADENOCARCINOMA; ANIMALS; BLOTTING, WESTERN; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; HOMEODOMAIN PROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; LENTIVIRUS; LUNG NEOPLASMS; MALE; MICE; MICE, NUDE; MIDDLE AGED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, SMALL INTERFERING; S PHASE; TRANSCRIPTION FACTORS; TUMOR BURDEN; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 84864361751     PISSN: 01715216     EISSN: 14321335     Source Type: Journal    
DOI: 10.1007/s00432-012-1206-2     Document Type: Article

References (22)

1. Arumugam T, Ramachandran V, Fournier KF et al (2009) Epithelial to mesenchymal transition contributes to drug resistance in pancreatic cancer. Cancer Res 69:5820-5828
2. Brummelkamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550-553 (Pubitemid 34408678)
3. Clarhaut J, Gemmill RM, Potiron VA et al (2009) (2009) ZEB-1, a repressor of the semaphorin 3F tumor suppressor gene in lung cancer cells. Neoplasia 11:157-166
4. Dohadwala M, Yang SC, Luo J et al (2006) Cyclooxygenase-2-dependent regulation of E-cadherin: prostaglandin E(2) induces transcriptional repressors ZEB1 and snail in non-small cell lung cancer. Cancer Res 66:5338-5345 (Pubitemid 43844959)
5. Gemmill RM, Roche J, Potiron VA et al (2011) ZEB1-responsive genes in non-small cell lung cancer. Cancer Lett 300:66-78
6. Ito M, Tanaka S, Kim S et al (2003) The specific expression of hypoxia inducible factor-1 alpha in human gastric mucosa induced by nonsteroidal anti-inflammatory drugs. Aliment Pharmacol Ther 18:90-98 (Pubitemid 36898004)
7. Kajita M, McClinic KN, Wade PA (2004) Aberrant expression of the transcription factors snail and slug alters the response to genotoxic stress. Mol Cell Biol 24:7559-7566 (Pubitemid 39121483)
8. Kokkinos MI, Wafai R, Wong MK et al (2007) Vimentin and epithelial- mesenchymal transition in human breast cancer-observations in vitro and in vivo. Cells Tissues Organs 185:191-203 (Pubitemid 46957486)
9. Lever AM, Strappe PM, Zhao J (2004) Lentiviral vectors. J Biomed Sci 11:439-449 (Pubitemid 38721027)
10. Li Y, Vandenboom TG II, Kong D et al (2009) Up-regulation of miR- 200 and let-7 by natural agents leads to the reversal of epithelialto- mesenchymal transition in gemcitabine-resistant pancreatic cancer cells. Cancer Res 69:6704-6712
11. Liu N, Bi F, Pan Y et al (2004) Reversal of the malignant phenotype of gastric cancer cells by inhibition of RhoA expression and activity. Clin Cancer Res 10:6239-6247 (Pubitemid 39287532)
12. Mejlvang J, Kriajevska M, Vandewalle C et al (2007) Direct repression of cyclin D1 by SIP1 attenuates cell cycle progression in cells undergoing an epithelial mesenchymal transition. Mol Biol Cell 18:4615-4624 (Pubitemid 350060186)
13. Milner AE, Levens JM, Gregory CD (1998) Flow cytometric methods of analyzing apoptotic cells. Methods Mol Biol 80:347-354
14. Parkin DM, Bray F, Ferlay J et al (2005) Global cancer statistics, 2002. CA Cancer J Clin 55:74-108 (Pubitemid 40372904)
15. Sayan AE, Griffiths TR, Pal R et al (2009) SIP1 protein protects cells from DNA damage-induced apoptosis and has independent prognostic value in bladder cancer. Proc Natl Acad Sci USA 106:14884-14889
16. Spaderna S, Schmalhofer O, Wahlbuhl M et al (2008) The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer. Cancer Res 68:537-544 (Pubitemid 351380082)
17. Takeyama Y, Sato M, Horio M et al (2010) Knockdown of ZEB1, a master epithelial-to-mesenchymal transition (EMT) gene, suppresses anchorage- independent cell growth of lung cancer cells. Cancer Lett 296:216-224
18. Travis W, Brambilla E, Müller-Hermelink K et al (2004) Tumours of the lung, pleura, thymus and heart. ARC Press, Lyon
19. Ulrich W, Jörg S, Ulrike C et al (2009) The EMT-activator ZEB1 promotes tumorigenicity by repressing stemness -inhibiting microRNAs. Nat Cell Biol 11:1487-1495
20. Vega S, Morales AV, Ocana OH et al (2004) Snail blocks the cell cycle and confers resistance to cell death. Genes Dev 18:1131-1143 (Pubitemid 38669034)
21. Yang S, Du J, Wang Z et al (2007) BMP-6 promotes E-cadherin expression through repressing deltaEF1 in breast cancer cells. BMC Cancer 7:211
22. Yuyen C, Chien C, Fang R et al (2010) 4β-Hydroxywithanolide E from Physalis peruviana (golden berry) inhibits growth of human lung cancer cells through DNA damage, apoptosis and G2/M arrest. BMC Cancer 10:46