Ribosome display

Methods in Molecular Biology

Volumn 901, Issue , 2012, Pages 101-116

  Thom, George ^a   Groves, Maria ^a  

^a MEDIMMUNE   (United States)

Author keywords

Escherichia coli S30 lysates; In vitro selection; Ribosome display; scFv

Indexed keywords

MESSENGER RNA;

ARTICLE; ESCHERICHIA COLI; GENETICS; METABOLISM; PEPTIDE LIBRARY; RIBOSOME;

ESCHERICHIA COLI; PEPTIDE LIBRARY; RIBOSOMES; RNA, MESSENGER;

ESCHERICHIA COLI;

EID: 84864139032     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-931-0_6     Document Type: Article

References (17)

1. Hanes J, Plückthun A (1997) In vitro selection and evolution of functional proteins by using ribosome display. Proc Natl Acad Sci U S A 94: 4937-4942
2. Hanes J, Schaffitzel C, Knappik A, Plückthun A (2000) Picomolar affinity antibodies from a fully synthetic naïve library selected and evolved by ribosome display. Nat Biotechnol 18:1287-1292
3. Lamla T, Erdmann V (2001) In vitro selection of other proteins than antibodies by means of ribosome display. FEBS Lett 502:35-40
4. Jermutus L, Honeggar A, Schwesinger F, Hanes J, Plückthun A (2001) Tailoring in vitro evolution for protein affinity or stability. Proc Natl Acad Sci U S A 98:75-80
5. Amstutz P, Pelletier JN, Guggisberg A (2002) In vitro selection for catalytic activity with ribosome display. J Am Chem Soc 124: 9396-9403
6. Matsuura T, Plückthun A (2004) Strategies for selection from protein libraries composed of de novo designed secondary structure modules. Orig Life Evol Biosph 34:151-157
7. Thom G, Cockroft AC, Buchanan AG et al (2006) Probing a protein-protein interaction by in vitro evolution. Proc Natl Acad Sci U S A 103:7619-7624
8. Zahnd C, Wyler E, Schwenk JM et al (2007) A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol 369: 1015-1028
9. Dower WJ, Cwirla SE (1992) Creating vast peptide expression libraries: Electroporation as a tool to construct plasmid libraries of greater than 109 recombinants. In: Chang DC et al (eds) Guide to electroporation and electrofusion. Academic, San Diego, CA, pp 291-301
10. Vaughan TJ, Williams AJ, Pritchard K et al (1996) Human antibodies with sub-nanomolar affinities isolated from a large nonimmunized phage display library. Nat Biotechnol 14: 309-314
11. Zubay G (1973) In vitro synthesis of protein in microbial systems. Annu Rev Genet 7: 267-287
12. Jermutus L, Ryabova LA, Plückthun A (1998) Recent advances in producing and selecting functional proteins by using cell-free translation. Curr Opin Biotechnol 9:534-548
13. Hajnsdorf E, Braun F, Haugel-Nielsen J et al (1996) Multiple degradation pathways of the rpsO mRNA of Escherichia coli RNase E interacts with the 5 ¢ and 3 ¢ extremities of the primary transcript. Biochemie 78:416-424
14. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185:60-89
15. Reynolds R, Bermudez-Cruz RM, Chamberlin MJ (1992) Parameters affecting transcription termination by Escherichia coli RNA polymerase. I. Analysis of 13 rho independent terminators. J Mol Biol 224:31-51
16. McCafferty J, Fitzgerald KJ, Earnshaw J et al (1994) Selection and rapid purification of murine antibody fragments that bind a transitionstate analog by phage display. Appl Biochem Biotechnol 47:157-171
17. Freedman RB, Hawkins HC, McLaughlin SH (1995) Protein disulfide-isomerase. Methods Enzymol 251:397-406