Genetic transformation of wheat: Current status and future prospects

Plant Biotechnology Reports

Volumn 6, Issue 3, 2012, Pages 183-193

  Li, Jiarui ^a,b   Ye, Xingguo ^a   An, Baoyan ^b   Du, Lipu ^a   Xu, Huijun ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b KANSAS STATE UNIVERSITY   (United States)

Author keywords

Genetic transformation; Techniques; Wheat

Indexed keywords

AGROBACTERIUM; RHIZOBIUM; TRITICUM AESTIVUM;

EID: 84864134877     PISSN: 18635466     EISSN: 18635474     Source Type: Journal    
DOI: 10.1007/s11816-011-0213-0     Document Type: Review

References (87)

1. Abranches R, Shultz RW, Thompson W, Allen GC (2005) Matrix attachment regions and regulated transcription increase and stabilize transgene expression. Plant Biotechnol J 3: 535-543.
2. Agarwal S, Loar S, Steber C, Zale J (2009) Floral transformation of wheat. Methods Mol Biol 478: 105-113.
3. Allen GC, Spiker S, Thompson WF (2000) Use of matrix attachment regions (MARs) to minimize transgene silencing. Plant Mol Biol 43: 361-376.
4. Altpeter F, Vasil V, Srivastava V, Stoger E, Vasil IK (1996a) Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep 16: 12-17.
5. Altpeter F, Vasil V, Srivastava V, Vasil IK (1996b) Integration and expression of the high molecular weight glutenin subunit 1Ax1 gene into wheat. Nat Biotechnol 14: 1155-1159.
6. Amoah BK, Wu H, Sparks C, Jones HD (2001) Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. J Exp Bot 52: 1135-1142.
7. Anand A, Zhou T, Trick HN, Gill BS, Bockus WW, Muthukrishnan S (2003) Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum. J Exp Bot 54: 1101-1111.
8. Barro F, Rooke L, Bekes F, Gras P, Tatham AS, Fido R, Lazzeri PA, Shewry PR, Barcelo P (1997) Transformation of wheat with high molecular weight subunit genes results in improved functional properties. Nat Biotechnol 15: 1295-1299.
9. Becker D, Brettschneider R, Lorz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J 5: 299-307.
10. Bhalla PL (2006) Genetic engineering of wheat-current challenges and opportunities. Trends Biotechnol 24: 305-311.
11. Blechl AE, Anderson OD (1996) Expression of a novel high-molecular-weight glutenin subunit gene in transgenic wheat. Nat Biotechnol 14: 875-879.
12. Chauhan H, Khurana P (2011) Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics. Plant Biotechnol J 9: 408-417.
13. Chen WP, Chen PD, Liu DJ, Kynast R, Friebe B, Velazhahan R, Muthukrishnan S, Gill BS (1999) Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene. Theor Appl Genet 99: 755-760.
14. Chen L, Zhang ZY, Liang HX, Liu HX, Du LP, Xu HJ, Xin ZY (2008) Overexpression of TiERF1 enhances resistance to sharp eyespot in transgenic wheat. J Exp Bot 59: 4195-4204.
15. Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol 115: 971-980.
16. Cheng M, Hu TC, Layton J, Liu CN, Fry JE (2003) Desiccation of plant tissues post-Agrobacterium infection enhances T-DNA delivery and increases stable transformation efficiency in wheat. In Vitro Cell Dev Biol Plant 39: 595-604.
17. Clemente T, Mitra A (2005) Genetic engineering of wheat: protocols and use to enhance stress tolerance. In: Liang GH, Skinner DZ (eds) Genetically modified crops: their development, uses, and risks. Haworth Press, New York, pp 131-163.
18. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16: 735-743.
19. Day CD, Lee E, Kobayashi J, Holappa LD, Albert H, Ow DW (2000) Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced. Genes Dev 14: 2869-2880.
20. De Block M, Debrouwer D (1991) Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium infection are mainly integrated at the same locus. Theor Appl Genet 82: 257-263.
21. Ding L, Li S, Gao J, Wang Y, Yang G, He G (2009) Optimization of Agrobacterium-mediated transformation conditions in mature embryos of elite wheat. Mol Biol Rep 36: 29-36.
22. Dong N, Liu X, Lu Y, Du LP, Xu HJ, Xin ZY, Zhang ZY (2010) Overexpression of TaPIEP1, a pathogen-induced ERF of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana. Funct Integr Genomics 10: 215-226.
23. Fahim M, Ayala-Navarrete L, Millar AA, Larkin PJ (2010) Hairpin RNA derived from viral NIa gene confers immunity to wheat streak mosaic virus infection in transgenic wheat plants. Plant Biotechnol J 8: 821-834.
24. Gadaleta A, Giancaspro A, Blechl A, Blanco A (2006) Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation. J Cereal Sci 43: 31-37.
25. Gao SQ, Xu HJ, Cheng XG, Chen M, Xu ZS, Li LC, Ye XG, Du LP, Hao XY, Ma YZ (2005) Improvement of wheat drought and salt tolerance by expression of a stress-inducible transcription factor GmDREB of soybean (Glycine max). China Sci Bull 50: 2714-2723.
26. Gao SQ, Chen M, Xia LQ, Xu HJ, Xu ZS, Li LC, Zhao CP, Cheng XG, Ma YZ (2009) A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat. Plant Cell Rep 28: 301-311.
27. Gao X, Zhang L, Zhou S, Wang C, Deng X, Zhang H, Yang G, Javeed H, He G (2011) AtMYB12 gene: a novel visible marker for wheat transformation. Mol Biol Rep 38: 183-190.
28. Godfray HC, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, Pretty J, Robinson S, Thomas SM, Toulmin C (2010) Food security: the challenge of feeding 9 billion people. Science 327: 812-818.
29. Greer MS, Kovalchuk I, Eudes F (2009) Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum. New Biotechnol 26: 44-52.
30. Harholt J, Bach IC, Lind-Bouquin S, Nunan KJ, Madrid SM, Brinch-Pedersen H, Holm PB, Scheller HV (2010) Generation of transgenic wheat (Triticum aestivum L.) accumulating heterologous endo-xylanase or ferulic acid esterase in the endosperm. Plant Biotechnol J 8(3): 351-362.
31. He Y, Jones HD, Chen S, Chen XM, Wang DW, Li KX, Wang DS, Xia LQ (2010) Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. J Exp Bot 61(6): 1567-1581.
32. Hess D, Dressler K, Nimnrichter R (1990) Transformation experiments by pipetting Agrobacterium into the spikelets of wheat. Plant Sci 72: 233-244.
33. Houben A, Schubert I (2007) Engineered plant minichromosomes: a resurrection of B chromosomes. Plant Cell 19: 2323-2327.
34. Hu T, Metz S, Chay C, Zhou HP, Biest N, Chen G, Chen M, Feng X, Radionenko M, Lu F, Fry J (2003) Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection. Plant Cell Rep 21: 1010-1019.
35. Janakiraman V, Steinau M, Mccoy SB, Trick HN (2002) Recent advances in wheat transformation. In Vitro Cell Dev Biol Plant 38: 404-414.
36. Janni M, Sella L, Favaron F, Blechl AE, De Lorenzo G, D'Ovidio R (2008) The expression of a bean PGIP in transgenic wheat confers increased resistance to the fungal pathogen Bipolaris sorokiniana. Mol Plant Microbe Interact 21: 171-177.
37. Jauhar PP (2006) Modern biotechnology as an integral supplement to conventional plant breeding: the prospects and challenges. Crop Sci 46: 1841-1849.
38. Jones HD (2005) Wheat transformation: current technology and applications to grain development and composition. J Cereal Sci 41: 137-147.
39. Jones HD, Doherty A, Wu H (2005) Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat. Plant Methods 1: 5.
40. Khanna HK, Daggard GE (2003) Agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium. Plant Cell Rep 21: 429-436.
41. Khurana J, Chugh A, Khurana P (2002) Regeneration from mature and immature embryos and transient gene expression via Agrobacterium-mediated transformation in emmer wheat (Triticum diccocum Schuble). Indian J Exp Biol 40: 1295-1303.
42. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10: 165-174.
43. Langridge P, Brettschneider R, Lazzeri P, Lorz H (1992) Transformation of cereals via Agrobacterium and the pollen pathway: a critical assessment. Plant J 2: 631-638.
44. Li JR, Zhao W, Li QZ, Ye XG, An BY, Li X, Zhang XS (2005) RNA silencing of Waxy gene results in low levels of amylose in the seeds of transgenic wheat (Triticum aestivum L.). Yi Chuan Xue Bao 32: 846-854.
45. Li Z, Zhou MP, Zhang ZY, Ren LJ, Du LP, Zhang BQ, Xu HJ, Xin ZY (2011) Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Funct Integr Genomics 11: 63-70.
46. Makandar R, Essig JS, Schapaugh MA, Trick HN, Shah J (2006) Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1. Mol Plant Microbe Interact 19: 123-129.
47. Mitic N, Nikolic R, Ninkovic S, Miljus-Djukic J, Neskovic M (2004) Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L. Biol Plant 48: 179-184.
48. Morino K, Olsen OA, Shimamoto K (1999) Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene. Plant J 17(3): 275-285.
49. Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with 2 distinct gene constructs. Plant J 5: 285-297.
50. Ogawa T, Kawahigashi H, Toki S, Handa H (2008) Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker. Plant Cell Rep 27: 1325-1331.
51. Okubara PA, Blechl AE, McCormick SP, Alexander NJ, Dill-Macky R, Hohn TM (2002) Engineering deoxynivalenol metabolism in wheat through the expression of a fungal trichothecene acetyltransferase gene. Theor Appl Genet 106: 74-83.
52. Ortiz JPA, Reggiardo MI, Ravizzin RA, Altabe SG, Cervigni GDL, Spitteler MA, Morata MM, Elias FE, VaUejos RH (1996) Hygromycin resistance as an efficient selectable marker for wheat stable transformation. Plant Cell Rep 15: 877-881.
53. Parrott DL, Anderson AJ, Carman JG (2002) Agrobacterium induces plant cell death in wheat (Triticum aestivum L.). Physiol Mol Pathol 60: 59-69.
54. Pellegrineschi A, Fennell S, McLean S, Brito RM, Velazquez L, Salgado M, Olivares JJ, Hernandez R, Hoisington D (2000) Routine transformation system for use with CIMMYT wheat varieties. In: Koholi M, Francis M (eds) Application of biotechnologies to wheat breeding. Proceedings of a conference at La Estanzuela, Uruguay, 19-20 November 1998, Montevideo, pp 111-120.
55. Pellegrineschi A, Noguera LM, Skovmand B, Brito RM, Velazquez L, Salgado MM, Hernandez R, Warburton M, Hoisington D (2002) Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants. Genome 45: 421-430.
56. Permingeat HR, Alvarez ML, Cervigni GDL, Ravizzini RA, Vallejos RH (2003) Stable wheat transformation obtained without selectable markers. Plant Mol Biol 52: 415-419.
57. Puchta H (2003) Marker-free transgenic plants. Plant Cell Tissue Org Cult 74: 123-134.
58. Regina A, Bird D, Topping D, Bowden S, Freeman J, Barsby T, Hashemi BK, Li Z, Rahman S, Morell M (2006) High amylose wheat generated by RNA-interference improves indices of large bowel health in rats. Proc Natl Acad Sci USA 103: 3546-3551.
59. Rooke L, Bekes F, Fido R, Barro F, Gras P, Tatham AS, Barcelo P, Lazzeri P, Shewry PR (1999) Overexpression of a gluten protein in transgenic wheat results in greatly increased dough strength. J Cereal Sci 30: 115-120.
60. Sestili F, Janni M, Doherty A, Botticella E, D'Ovidio R, Masci S, Jones HD, Lafiandra D (2010) Increasing the amylose content of durum wheat through silencing of the SBEIIa genes. BMC Plant Biol 10: 144.
61. Shirley S, Xing A, Ye X, Schweiger B, Kinney A, Graef G, Clemente T (2004) Production of c-linolenic acid and stearidonic acid in seeds of marker-free transgenic soybean. Crop Sci 44: 646-652.
62. Srivastava V, Ow DW (2004) Marker-free site-specific gene integration in plants. Trends Biotechnol 22: 627-629.
63. Stoykova P, Stoeva-Popova P (2011) PMI (manA) as a nonantibiotic selectable marker gene in plant Biotechnology. Plant Cell Tissue Organ Cult 105: 141-148.
64. Supartana P, Shimizu T, Nogawa M, Shioiri H, Nakajima T, Haramoto N, Nozue M, Kojima M (2006) Development of simple and efficient in planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens. J Biosci Bioeng 102: 162-170.
65. Takumi S, Shimada T (1996) Production of transgenic wheat through particle bombardment of scutellar tissues: frequency is influenced by culture duration. J Plant Physiol 149: 418-423.
66. Tamás C, Kisgyörgy BN, Rakszegi M, Wilkinson MD, Yang MS, Láng L, Tamás L, Bedo Z (2009) Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality. Plant Cell Rep 28: 1085-1094.
67. Tao LL, Yin GX, Du LP, Shi ZY, She MY, Xu HJ, Ye XG (2011) Improvement of plant regeneration from immature embryos of wheat infected by Agrobacteriumtumefaciens. Agric Sci China 10: 317-326.
68. Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Nat Biotechnol 10: 667-674.
69. Vaucheret H, Fagrad M (2001) Transcriptional gene silencing in plants: targets, inducers and regulators. Trends Genet 17: 29-35.
70. Vishnudasan D, Tripathi MN, Rao U, Khurana P (2005) Assessment of nematode resistance in wheat transgenic plants expressing potato proteinase inhibitor (PIN2) gene. Transgenic Res 14: 665-675.
71. Wang YL, Xu MX, Yin GX, Tao LL, Wang DW, Ye XG (2009) Transgenic wheat plants derived from Agrobacterium-mediated transformation of mature embryo tissues. Cereal Res Commun 37: 1-12.
72. Weeks JT, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102: 1077-1084.
73. Witrzens B, Brettell RI, Murray ER, McElroy D, Li Z, Dennis ES (1998) Comparison of three selectable marker genes for transformation of wheat by microprojectile bombardment. Aust J Plant Physiol 25: 39-44.
74. Wright M, Dawson J, Dunder E, Suttie J, Reed J, Kramer C, Chang Y, Novitzky R, Wang H, Artim-Moore L (2001) Efficient biolistic transformation of maize (Zea mays L.) and wheat (Triticum aestivum L.) using the phosphomannose isomerase gene, pmi, as the selectable marker. Plant Cell Rep 20: 429-436.
75. Wu HX, Doherty A, Jones HD (2008) Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. Durum) using additional virulence genes. Trans Res 17: 425-436.
76. Wu H, Doherty A, Jones HD (2009) Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos. Methods Mol Biol 478: 93-103.
77. Xing A, Zhang Z, Shirley S, Paul S, Tom C (2000) The use of the two T-DNA binary system to drive marker-free transgenic soybeans. In Vitro Cell Dev Biol Plant 36: 456-463.
78. Yao Q, Cong L, Chang JL, Li KX, Yang GX, He GY (2006) Low copy number gene transfer and stable expression in a commercial wheat cultivar via particle bombardment. J Exp Bot 57: 3737-3746.
79. Yao Q, Cong L, He G, Chang J, Li K, Yang G (2007) Optimization of wheat co-transformation procedure with gene cassettes resulted in an improvement in transformation frequency. Mol Biol Rep 34: 61-67.
80. Yin GX, Wang YL, She MY, Du LP, Xu HJ, Ma JX, Ye XG (2011) Establishment of a highly efficient regeneration system for the mature embryo culture of wheat. Agric Sci China 10: 9-17.
81. Yu WC, Han FP, Gao Z, Vega JM, Birchler JA (2007) Construction and behavior of engineered minichromosomes in maize. Proc Natl Acad Sci USA 104: 8924-8929.
82. Zale JM, Agarwal S, Loar S, Steber CM (2009) Evidence for stable transformation of wheat by floral dip in Agrobacterium tumefaciens. Plant Cell Rep 28: 903-913.
83. Zhang H, Zhu JK (2011) RNA-directed DNA methylation. Curr Opin Plant Biol 14: 142-147.
84. Zhang S, Rybszynski JJ, Landenberg WG, Mitra A, French R (2000) An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration. Plant Cell Rep 18: 959-966.
85. Zhao TJ, Zhao SY, Chen HM, Zhao QZ, Hu ZM, Hou BK, Xia GM (2006) Transgenic wheat progeny resistant to powdery mildew generated by Agrobacterium inoculum to the basal portion of wheat seedling. Plant Cell Rep 25: 1199-1204.
86. Zhou H, Arrowsmith JW, Fromm ME (1995) Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Rep 15: 159-163.
87. Zhou H, Berg JD, Blank SE, Chay CA, Chen G, Eskelsen SR, Fry JE, Hoi S, Hu T, Isakson PJ, Lawton MB, Metz SG, Rempel CB, Ryerson DK, Sansone AP, Shook AL, Starke RJ, Tichota JM, Valenti SA (2003) Field efficacy assessment of transgenic roundup ready wheat. Crop Sci 43: 1072-1075.