Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos

Methods in Molecular Biology

Volumn 478, Issue , 2009, Pages 93-103

  Huixia, Wu ^b   Angela, Doherty ^a   Jones, Huw D ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

^b INTERNATIONAL MAIZE AND WHEAT IMPROVEMENT CENTER CIMMYT   (Mexico)

Author keywords

AGL1; Agrobacterium tumefaciens; Bread wheat; Durum wheat; Immature embryo; Inoculation

Indexed keywords

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; RHIZOBIUM; TRITICUM AESTIVUM; TRITICUM TURGIDUM SUBSP. DURUM;

EID: 60349092426     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-379-0_5     Document Type: Article

References (11)

1. Broothaerts, W., Mitchell, H. J., Weir, B., Kaines, S., Smith, L. M. A., Yang, W., Mayer, J. E., Roa-Rodriguez, C. and Jefferson, R. A. (2005) Gene transfer to plants by diverse species of bacteria. Nature 433, 629-633.
2. Jones, H. D., Doherty, A. and Wu, H. (2005) Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat. Plant Methods 1, 5.
3. Lazo, G. R., Stein, P. A. and Ludwig, R. A. (1991) A DNA Transformation-competent Arabidopsis genomic library in Agrobacterium. Bio-Technology 9, 963 967.
4. Wu, H., Sparks, C., Amoah, B. and Jones, H. D. (2003) Factors influencing successful Agrobacterium-mediated genetic transformation of wheat. Plant Cell Rep. 21, 659-668.
5. Hellens, R. P., Edwards, E. A., Leyland, N. R., Bean, S. and Mullineaux, P. M.(2000)pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol. Biol. 42, 819-832.
6. Amoah, B. K., Wu, H., Sparks, C. and Jones, H. D. (2001) Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. J. Exp. Bot. 52, 1135-1142.
7. Wu, H., Doherty, A. and Jones, H. D. (2008) Efficient and rapid Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. ssp durum) using additional virulence genes. Transgen. Res. 17, 425-436.
8. Sparks, C. A. and Jones, H. D. (2004) Transformation of wheat by biolistics, in Transgenic Crops of the World Essential Protocols, (Curtis, I. P., ed.), Kluwer, Dordrecht, pp. 19-35.
9. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, New York.
10. Tingay, S., McElroy, D., Kalla, R., Fieg, S., Wang, M. B., Thornton, S. and Brettell, R. (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J. 11, 1369-1376.
11. Wu, H., Sparks, C. A. and Jones, H. D. (2006) Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium-mediated transformation. Mol. Breed. 18, 195-208.