Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum

Journal of Experimental Botany

Volumn 54, Issue 384, 2003, Pages 1101-1111

  Anand, Ajith ^a   Zhou, Tian ^a   Trick, Harold N ^a   Gill, Bikram S ^a   Bockus, William W ^a   Muthukrishnan, Subbaratnam ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

1,3 glucanase; Chitinase; Field evaluation; Genetic transformation; Transgene silencing; Wheat scab

Indexed keywords

BIOASSAY; GENES; GRAIN (AGRICULTURAL PRODUCT); GREENHOUSE EFFECT; PROTEINS;

BIOLISTIC TRANSFORMATION PROTOCOL;

PLANTS (BOTANY);

BETA-GLUCOSIDASE; CHITINASE; CROPS, AGRICULTURAL; ENVIRONMENT, CONTROLLED; FUSARIUM; GENE EXPRESSION; GENE SILENCING; GENETIC COMPLEMENTATION TEST; GLUCAN 1,3-BETA-GLUCOSIDASE; IMMUNITY, NATURAL; PLANT DISEASES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; SWEETENING AGENTS; TRANSGENES; TRITICUM;

EUKARYOTA; FUNGI; FUSARIUM; GIBBERELLA ZEAE; TRITICUM AESTIVUM;

EID: 0037336601     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/erg110     Document Type: Article

References (44)

1. Altpeter F, Vasil V, Srivastava V, Stöger E, Vasil IK. 1996. Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Reports 16, 12-17.
2. Anderson JA, Stack RW, Liu S, Waldron BL, Fjed AD, Coyne C, Moreno-Sevilla B, Mitchell JF, Song QJ, Froberg RC. 2001. DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theoretical and Applied Genetics 102, 1164-1168.
3. Bai GH, Shaner G. 1994. Scab of wheat: prospects for control. Plant Disease 78, 760-766.
4. Barro F, Rooke L, Bekes F, Gras P, Tatham AS, Fido F, Lazzeri PA, Shewry PR, Barcelo P. 1997. Transformation of wheat with high molecular weight glutenin subunit genes results in improved functional properties. Nature Biotechnology 15, 1295-1299.
5. Bieri S, Potrykus I, Futterer J. 2000. Expression of active barley seed ribosome-inactivating protein in transgenic wheat. Theoretical and Applied Genetics 100, 755-763.
6. Bliffeld M, Mundy J, Potrykus I, Futterer J. 1999. Genetic engineering of wheat for increased resistance to powdery mildew disease. Theoretical and Applied Genetics 98, 1079-1086.
7. Broglie K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais J, Broglie RM. 1991. Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254, 1194-1197.
8. Chen WP, Chen PD, Liu DJ, Kynast RJ, Friebe B, Velazhahan R, Muthukrishnan S, Gill BS. 1999. Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene. Theoretical and Applied Genetics 99, 755-760.
9. Chen WP, Gu X, Liang GH, Muthukrishnan S, Chen PD, Liu DJ, Gill BS. 1998. Introduction and constitutive expression of a rice chitinase gene in bread wheat using biolistic bombardment and the bar gene as a selectable marker. Theoretical and Applied Genetics 97, 1296-1306.
10. Christensen AH, Schrrock RA, Quail PH. 1992. Maize ubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Molecular Biology 18, 675-689.
11. Clausen M, Krauter R, Schachermayr G, Potrykus I, Sautter C. 2000. Antifungal activity of a virally encoded gene in transgenic wheat. Nature Biotechnology 18, 446-449.
12. Datta K, Muthukrishnan S, Datta SK. 1999. Expression and function of PR-protein genes in transgenic plants. In: Datta SK, Muthukrishnan S, eds. Pathogenesis-related proteins in plants. Boca Raton, FL: CRC Press, 261-277.
13. Finer JJ, Vain P, Jones MW, McMullen MD. 1992. Development of the particle inflow gun for DNA delivery to plant cells. Plant Cell Reports 11, 323-328.
14. Gamborg OL, Miller RA, Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50, 151-158.
15. Igaz P, Fejer G, Szalai C, Toth S, Falus A. 1998. Development of competitive mRNA PCR for the quantification of interleukin-6-responsive junB oncogene expression. BioTechniques 24, 854-860.
16. Jach G, Gornhardt B, Mundy J, Logemann J, Pinsdorf E, Leah R, Schell J, Maas C. 1995. Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. The Plant Journal 8, 97-109.
17. Jongedijk E, Tigelaar H, van Roekel JSC, Bres-Vloemans SA, Dekker I, van den Elzen P JM, Cornelissen BJC, Melchers LS. 1995. Synergistic activity of β-1,3-glucanases and chitinases enhances fungal resistance in transgenic tomato plants. Euphytica 85, 173-180.
18. Leah R, Tommerup H, Svendsen I, Mundy J. 1991. Biochemical and molecular characterization of three barley seed proteins with anti-fungal properties. Journal Biological Chemistry 266, 1464-1473.
19. Leckband G, Lorz H. 1998. Transformation and expression of a stilbene synthase gene of Vitis vinifera L. in barley and wheat for increased fungal resistance. Theoretical and Applied Genetics 96, 1004-1012.
20. Li WL, Faris JD, Muthukrishnan S, Liu DJ, Chen PD, Gill BS. 2001. Isolation and characterization of novel cDNA clones of acidic chitinases and β-1,3-glucanases from wheat spikes infected with Fusarium graminearium. Theoretical and Applied Genetics 102, 353-362.
21. Lin W, Anuratha CS, Datta K, Potrykus I, Muthukrishnan S, Datta SK. 1995. Genetic engineering of rice for resistance to sheath blight. Bio/Technology 13, 686-691.
22. Mauch F, Mauch-Mani B, Boller T. 1988. Antifungal hydrolases in pea tissue: inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiology 88, 936-942.
23. McMullen M, Jones R, Gallenberg D. 1997. Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Disease 81, 1340-1348.
24. Melchers LS, Stuiver MH. 2000. Novel genes for disease-resistance breeding. Current Opinion in Plant Biology 3, 147-152.
25. Mohammadi M, Kazemi H. 2002. Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Science 162, 491-498.
26. Murashige T, Skoog FA. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology 15, 473-497.
27. Neuhaus JM, Ahl-Goy P, Hinz U, Flores S, Meins FJ. 1991. High-level expression of a tobacco chitinase gene in Nicotiana sylvestris: susceptibility of transgenic plants to Cercospora nicotianae infection. Plant Molecular Biology 16, 141-151.
28. Oldach KH, Becker D, Lorz H. 2001. Heterologous expression of genes mediating enhanced fungal resistance in transgenic wheat. Molecular Plant Microbe Interactions 14, 832-838.
29. Parry DW, Jenkinson P, McLeod L. 1995. Fusarium ear blight (scab) in small grain cereals - a review. Plant Pathology 44, 207-238.
30. Patnaik D, Khurana P. 2001. Wheat biotechnology: a minireview. European Journal of Biotechology 4, 1-29.
31. Pritsch C, Muelbauer GJ, Bushnell WR, Somers DA, Vance CR. 2000. Fungal development and induction of defence response genes during early infection of wheat spikes by Fusarium graminearum. Molecular Plant Microbe Interactions 13, 159-169.
32. Pritsch C, Vance CP, Bushnell RW, Somers AD, Hohn TM, Muehlbauer GJ. 2001. Systemic expression of defence response genes in wheat spikes as a response to Fusarium graminearum infection. Physiological and Molecular Plant Pathology 58, 1-12.
33. SAS Institute. 1998. System Version 7 for Windows. Cary, NC: SAS Institution.
34. Schweizer P, Christoffel A, Dudler R. 1999. Transient expression of members of the germin-like gene in epidermal cells of wheat confers disease resistance. The Plant Journal 20, 541-552.
35. Shah DM. 1997. Genetic engineering for fungal and bacterial diseases. Current Opinion in Biotechology 8, 208-214.
36. Shaner G, Buechley G. 1999. Control of wheat diseases with foliar fungicides, 1998. Fungicide and Nematicide Tests 54, 337-338.
37. Sharp GL, Martin JM, Lanning SP, Blake NK, Brey CW, Sivamani E, Qu R, Talbert LE. 2002. Field evaluation of transgenic and classical sources of wheat streak mosaic virus resistance. Crop Science 42, 105-110.
38. Swegle M, Kramer J, Muthukrishnan S. 1992. Properties of barley seed chitinase and release of embryo-associated isoforms during early stages of inhibition. Plant Physiology 99, 1009-1014.
39. Vigers AJ, Roberts WK, Selitrennikoff CP. 1991. A new family of plant antifungal proteins. Molecular Plant Microbe Interactions 4, 315-323.
40. Xu Y, Chen L. 1993. Wheat scab; theory and practice of control (in Chinese). Jiangsu, China: Science and Technology Press of Jiangsu, 201-216.
41. Yun DJ, Ibeas JI, Lee H, Coca MA, Narasimhan ML, Uesono Y, Hasegawa PM, Pardo JM, Bressan RA. 1998. Osmotin, a plant antifungal protein, subverts signal transduction to enhance fungal cell susceptibility. Molecular Cell 1, 807-812.
42. Zhang L, French R, Langenberg WG, Mitra A. 2001. Accumulation of barley stripe mosaic virus is significantly reduced in transgenic wheat plants expressing a bacterial ribonuclease. Transgenic Research 10, 13-19.
43. Zhou H, Arrowsmith JW, Fromm ME, Hironaka CM, Taylor ML, Rodriguez D, Pajeau ME, Brown SM, Santino CG, Fry JE. 1995. Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Reports 15, 159-163.
44. Zhu Q, Maher EA, Maced M, Dixon RA, Lamb C. 1994. Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase genes in transgenic tobacco. Bio/Technology 12, 807-812.