RNA silencing of waxy gene results in low levels of amylose in the seeds of transgenic wheat (Triticum aestivum L.)

Acta Genetica Sinica

Volumn 32, Issue 8, 2005, Pages 846-854

  Li, Jia Rui ^a   Zhao, Wei ^a,b   Li, Quan Zi ^a   Ye, Xing Guo ^b   An, Bao Yan ^a   Li, Xiang ^a   Zhang, Xian Sheng ^a  

^a SHANDONG AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Agrobacterium mediated transformation; Amylose; RNA interference; Waxy; Wheat

Indexed keywords

AMYLOSE; COMPLEMENTARY DNA; MESSENGER RNA; STARCH SYNTHASE; UNCLASSIFIED DRUG; WAXY PROTEIN;

ARTICLE; GENE; GENE SILENCING; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PLANT SEED; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SOUTHERN BLOTTING; TRANSGENIC PLANT; WAXY GENE; WHEAT;

AMYLOSE; BLOTTING, NORTHERN; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHIZOBIUM; RNA INTERFERENCE; RNA, MESSENGER; SEEDS; STARCH SYNTHASE; TRANSFORMATION, GENETIC; TRITICUM;

AGROBACTERIUM; TRITICUM AESTIVUM; ZEA MAYS;

EID: 23944491365     PISSN: 03794172     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. Smith A M, Martin C. Starch biosynthesis and the potential for its manipulation. In: Grierson D, eds. Plant Biotechnology (Vol 3). Glasgow, Blackie Academic & Professional, 1993, 1-54.
2. Ball S, Guan H P, James M, Myers A, Keeling P, Mouille G, Buléon A, Colonna P, Preiss J. From glycogen to amylopectin: a model for the biogenesis of the plant starch granule. Cell, 1996, 86: 349-352.
3. Preiss J. Biology and molecular biology of starch synthesis and its regulation. Plant Mol Cell Biol, 1990, 7: 59-114.
4. Nelson O E, Rines H W. The enzymatic deficiency in the waxy mutant of maize. Biochem Biophys Res Commun, 1962, 9: 297-300.
5. Klǒsgen R B, Gierl A, Schwarz-Sommer Z, Saedler H. Molecular analysis of the Waxy locus of Zea mays. Mol Gen Genet, 1986, 203: 237-244.
6. Ronde W, Becker D, Salamini F. Structural analysis of the Waxy locus from Hordeum vulgare. Nucleic Acids Res, 1988, 16: 7185-7186.
7. Wang Z, Wu Z, Xing Y, Zheng F, Guo X, Zhang W, Hong M. Nucleotide sequence of rice Waxy gene. Nucleic Acids Res, 1990, 18: 5898.
8. Van der Leij F R, Visser R G F, Ponstein A S, Jacobsen E, Feenstra W J. Sequence of the structural gene for granule-bound starch synthase of potato (Solanum tuberosum L.) and evidence for a single point deletion in the amf allele. Mol Gen Genet, 1991, 228: 240-248.
9. Dry I, Smith A M, Edwards A, Bhattacharyya M, Dunn P, Martin C. Characterization of cDNAs encoding two isoforms of granule-bound starch synthase which show differential expression in developing storage organs of pea and potato. Plant J, 1992, 2: 193-202.
10. Ainsworth C, Clark J, Balsdon J. Expression, organization and structure of the genes encoding the waxy protein (granule-bound starch synthase) in wheat. Plant Mol Biol, 1993, 22: 67-82.
11. Chao S, Sharp P J, Worland A J, Warham E J, Koebner R M D, Gale M D. RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor Appl Genet, 1989, 78: 495-504.
12. Miura H, Sugawara A. Dosage effects of the three Wx genes on amylose synthesis in wheat endosperm. Theor Appl Genet, 1996, 93: 1066-1070.
13. Fujita N, Taira T. A 56-kDa protein is a novel granule-bound starch synthase existing in the pericarps, aleurone layers, and embryos of immature seed in diploid wheat (Triticum monococcum L.). Planta, 1998, 207: 125-132.
14. Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T. Production of waxy (amylose-free) wheats. Mol Gen Genet, 1995, 248: 253-259.
15. Van der Krol A R, Mol J N, Stuitje A R. Modulation of eukaryotic gene expression by complementary RNA or DNA sequences. Biotechniques, 1988, 10: 958-976.
16. Muller-Rober B, Sonnewald U, Willmitzer L. Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes. EMBO J, 1992, 4: 1229-1238.
17. Anja G J K, Evert J, Richard G F V. Formation and deposition of amylose in the patato tuber starch granule are affected by the reduction of granule-bound starch synthase gene expression. Plant cell, 1994, 6: 43-52.
18. Rie T, Midori N, Masayuki I, Ron J O, Susan R W, Ko S. Antisense waxy genes with highly active promoters effectively suppress waxy gene expression in transgenic rice. Plant Cell Physiol, 2000, 41(7): 881-888.
19. Liu Q Q, Wang Z Y, Chen X H, Cai X L, Tang S Z, Yu HX, Zhang J L, Hong M M, Gu M H. Stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. Transgenic Research, 2003, 12: 71-82.
20. Fire A, Xu S, Montgomery M K, Kostas S A, Driver S E, Mello C C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 1998, 391: 806-811.
21. Bernstein E, Caudy A A, Hammond S M, Hannon G J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature, 2001, 409: 363-366.
22. Liu Q H, Rand T A, Kalidas S, Du F, Kim H E, Smith D P, Wang X D. R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway. Science, 2003, 26: 1921-1925.
23. Matzke M A, Mette M F, Matzke A J. Transgenic silencing by the host genome defense: implications for the evolution of epigenetic control mechanisms in plants and vertebrates. Plant Mol Biol, 2000, 43: 401-415.
24. Lawrence R J, Pikaard C S. Transgene-induced RNAi: a strategy for overcoming gene redundancy in polyploids to generate loss-of-function mutations. Plant J, 2003, 36: 114 -121.
25. Delloporta S L, Wood J, Hicks J B. A plant DNA minipreparation: version II. Plant Mol Biol Rep, 1983, 4: 19.
26. Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1989.
27. Cheng M, Fry J E, Pang S, Zhou H, Hironaka C, Duncan D R, Conner T W, Wan Y. Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiology, 1997, 115(3): 971-980.
28. HE Zhao-Fan. Grain Seed Quality and It's Analysis Technology. Beijing: Chinese Agricultural Publisher, 1985, 274-294.
29. Murai J, Taira T, Ohta D. Isolation and characterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat. Gene, 1999, 1: 71-79.
30. Van L L, Mrinal B, Robert F. The genes encoding granule-bound starch synthese at the waxy loci of the A, B,and D progenitors of common wheat. Genome, 1999, 43: 264-272.
31. Mooney P A, Goodwin P B, Dennis E S, Llewellyn D J. Agrobacterium tumefaciens-gene transfer into wheat tis- sues. Plant Cell Tissue Organ Cult, 1991, 25: 209-218.
32. Hiei Y, Ohta S, Komari T, Kumashiro T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium tumeficience and sequence analysis of the boundaries of the T-DNA. Plant J, 1994, 6: 271-282.
33. Gould J, Devery M, Hasegawa O, Ulian E C, Peterson G, Smith R H. Transformation of Zea mays L. using Agrobacterium tumeficience and the shoot apex. Plant Physiol, 1991, 95(2): 426-434.
34. Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumeficience. Nat Biotechnol, 1996, 14: 745-750.
35. Tingay S, McElroy D, Kalla R, Fieg S A, Wang M, Thornton S, Brettell R. Agrobacterium tumefaciens-mediated barley transformation. Plant J, 1997, 11: 1369-1376.
36. Hu T, Metz S, Chay C, Zhou H P, Biest N, Chen G, Cheng M, Feng X, Radionenko M, Lu F, Fry J. Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection. Plant Cell Rep, 2003, 21: 1010-1019.
37. Anand A, Zhou T, Trick H N, Gill B S, Bockus W W, Muthukrishnan S. Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum. J Exp Bot, 2003, 54: 1101-1111.
38. Altpeter F, Vasil V, Srivastava V, Stöger E, Vasil I K. Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Reports, 1996, 16: 12-17.
39. Miki D, Shimamoto K. Simple RNAi vectors for stable and transient suppression of gene function in rice. Plant Cell Physiol, 2004, 45(4): 490-495.