Comparison of yeast cell protein solubilization procedures for two- dimensional electrophoresis

Electrophoresis

Volumn 20, Issue 4-5, 1999, Pages 826-829

  Harder, Alois ^a   Wildgruber, Robert ^a   Nawrocki, Arek ^b   Fey, Stephen J ^b   Larsen, Peter Mose ^b   Görg, Angelika ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Immobilized pH gradient; Saccharomyces cerevisiae; Two dimensional polyacrylamide gel electrophoresis; Yeast protein solubilization

Indexed keywords

ELECTROPHORESIS; METABOLISM; PROTEINS; SODIUM DODECYL SULFATE; SOLUBILITY; THIOUREAS; UREA;

CELL PROTEINS; IMMOBILIZED PH GRADIENT; LYSIS BUFFER; PH GRADIENTS; PROTEIN DEGRADATION; SODIUM DODECYL SULPHATE; SOLUBILISATION; TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; YEAST PROTEIN SOLUBILIZATION; YEAST PROTEINS;

YEAST;

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; BUFFER; DITHIOTHREITOL; DODECYL SULFATE SODIUM; FUNGAL PROTEIN; THIOUREA; UREA;

CONFERENCE PAPER; CONTROLLED STUDY; CYTOLYSIS; MOLECULAR WEIGHT; NONHUMAN; PH; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN MODIFICATION; SACCHAROMYCES CEREVISIAE; SOLUBILIZATION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 0032935808     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(19990101)20:4/5<826::AID-ELPS826>3.0.CO;2-A     Document Type: Conference Paper

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