A simple and efficient method for processing of cell lysates for two-dimensional gel electrophoresis

Electrophoresis

Volumn 31, Issue 14, 2010, Pages 2429-2435

  Xavier, Tessy ^a   Sreepriya, ^a   Ganesan, Trivadi S ^a   Menon, Krishnakumar N ^a  

^a AMRITA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

2 DE; Cell lysate; Centri con; Sample preparation

Indexed keywords

ACETONE; CELLS; CYTOLOGY; ELECTROPHORESIS; METABOLISM; NUCLEIC ACIDS; QUALITY CONTROL; SOLUBILITY; THIOUREAS; UREA;

2-DE; CELL LYSATES; CELLULAR PROTEINS; CENTRI-CON; SAMPLE PREPARATION; SIMPLE++; SOLUBILISATION; THIOUREA SOLUTIONS; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

PROTEINS;

AMMONIUM ACETATE; CELL PROTEIN; CHLOROFORM; ISOPENTYL ALCOHOL; METHANOL; PHENOL; PROTEINASE; THIOUREA; UREA; ACETONE; ALCOHOL; PROTEIN;

ARTICLE; CELL LYSATE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DENATURATION; HUMAN; HUMAN TISSUE; PRECIPITATION; PROTEIN ANALYSIS; PROTEOMICS; QUALITATIVE ANALYSIS; SOLUBILIZATION; TWO DIMENSIONAL GEL ELECTROPHORESIS; ULTRASOUND; CELL LINE; CHEMISTRY; ECONOMICS; EVALUATION STUDY; ISOLATION AND PURIFICATION; PROCEDURES;

ACETONE; CELL LINE; CHEMICAL PRECIPITATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ETHANOL; HUMANS; PROTEINS; PROTEOMICS; THIOUREA; UREA;

EID: 77954691758     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200900644     Document Type: Article

References (28)

1. Smithies, O., Poulik, M. D., Nature 1956, 177, 1033.
2. O'Farrell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
3. Klose, J., Humangenetik 1975, 26, 231-243.
4. Görg, A., Obermaier, C., Boguth, G., Harder, A., Scheibe, B., Wildgruber, R., Weiss, W., Electrophoresis 2000, 21, 1037-1053.
5. Klose, J., Kobalz, U., Electrophoresis 1995, 16, 1034-1059.
6. Righetti, P. G., Gianazza, E., Bjellgvist, B., J. Biochem. Biophys. Methods 1983, 8, 89-108.
7. Ornstein, D. K., Tyson,D.R., Urol. Oncol. 2006, 24, 231-236.
8. Matt, P., Fu, Z., Fu, Q., Van Eyk, J. E., Physiol. Genomics 2008, 14, 12-17.
9. Gallego-Delgado, J., Lazaro, A., Osende, J. I., Barderas, M. G., Blanco-Colio, L. M., Duran, M. C., Martin-Ventura, J. L., Vivanco, F., Egido, J., Kidney Int. Suppl. 2005, 99, S103-S107.
10. Hu, S., Xie, Y., Ramachandran, P., Ogorzalek Loo, R. R., Li, Y., Loo, J. A., Wong, D. T., Proteomics 2005, 5, 1714-1728.
11. Wu, Y., Zhou, J., Zhang, X., Zheng, X., Jiang, X., Shi, L., Yin, W., Wang, J., Proteome Sci. 2009, 7, 38.
12. Rabilloud, T., Electrophoresis 1996, 17, 813-829.
13. Stagsted, J., Bendixen, E., Andersen, H. J., J. Agric. Food Chem. 2004, 52, 3967-3974.
14. Herbert, B. R., Molloy, M. P., Gooley, A .A., Walsh, B. J., Bryson, W. G., Williams, K. L., Electrophoresis 1998, 19, 845-851.
15. Rabilloud, T., Methods Mol. Biol. 2009, 528, 259-267.
16. Rabilloud, T., Adessi, C., Giraudel, A., Lunardi, J., Electrophoresis 1997, 18, 307-316.
17. Herbert, B., Electrophoresis 1999, 20, 660-663.
18. Molloy, M. P., Anal. Biochem. 2000, 280, 1-10.
19. Antonioli, P., Bachi, A., Fascoli, E., Righetti, P. G., J. Chromatogr. A 2009, 1216, 3606-3612.
20. Berkelman, T., Methods Mol. Biol. 2008, 424, 51-62.
21. Mastro, R., Hall, M., Anal. Biochem. 1999, 10, 313-315.
22. Centlow, M., Hansson, S. R., Welinder, C., J. Biomed. Biotechnol. 2010, doi. 10.1155/2010/458748
23. Askonas, B. A., Biochem. J. 1951, 48, 42-48.
24. Vogelstein, B., Gillespie, D., Proc. Natl. Acad. Sci. USA 1979, 76, 615-619.
25. Osborne, M. R., Phillips, D. H., Chem. Res. Toxicol. 2000, 13, 257-261.
26. Wang, H., Hanash, S., Mass Spectrom. Rev. 2005, 24, 413-426.
27. Kirkland, P. A., Busby, J., Stevens, S., Jr., Maupin-Furlow, J. A., Anal. Biochem. 2006, 351, 254-259.
28. Shaw, M. M., Riederer, B. M., Proteomics 2003, 3, 1408-1417.