Performance comparison of protein A affinity resins for the purification of monoclonal antibodies

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 848, Issue 1, 2007, Pages 97-107

  Swinnen, K ^a   Krul, A ^a   Van Goidsenhoven, I ^a   Van Tichelt, N ^a   Roosen, A ^a   Van Houdt, K ^a  

^a Biosciences and Technology Department   (Belgium)

Author keywords

Affinity chromatography; Breakthrough curve; Design of experiments; DOE; Dynamic binding capacity; Monoclonal antibody; Packed bed absorption; PBA; Process robustness; Production rate; Protein A; Purification

Indexed keywords

BREAKTHROUGH CURVES; DYNAMIC BINDING CAPACITY; PACKED BED ABSORPTION; PROCESS ROBUSTNESS; PRODUCTION RATE;

AFFINITY CHROMATOGRAPHY; BINDING ENERGY; MATHEMATICAL MODELS; MONOCLONAL ANTIBODIES; PURIFICATION; RESINS;

PROTEINS;

IMMUNOGLOBULIN G; IMMUNOGLOBULIN G4 MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY; PROTEIN A; RESIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); COST; DRUG PURIFICATION; FLOW RATE; LEACHING; MATHEMATICAL MODEL; MOUSE; NONHUMAN; PRIORITY JOURNAL; VOLUMETRY;

ALGORITHMS; ANIMALS; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN G; MICE; REPRODUCIBILITY OF RESULTS; STAPHYLOCOCCAL PROTEIN A;

EID: 33847780753     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2006.04.050     Document Type: Article

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