Antibody purification by affinity chromatography based on small molecule affinity ligands identified by SPR-based screening of chemical microarrays

Journal of Chromatography A

Volumn 1218, Issue 29, 2011, Pages 4649-4659

  Arnold, Marc ^a   Bittermann, Holger ^a   Kalbfuss Zimmermann, Bernd ^a   Neumann, Thomas ^a   Schmidt, Kristina ^a   Sekul, Renate ^a   Hilbrig, Frank ^b   Ludolph, Heiko ^b   Freitag, Ruth ^b  

^a Graffinity Pharmaceutical Design GmbH   (Germany)

^b UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Affinity chromatography; Antibody purification; Chemical microarrays; Small molecule affinity ligands

Indexed keywords

AFFINITY CONSTANTS; AFFINITY LIGANDS; ANTIBODY CAPTURE; ANTIBODY PURIFICATION; BINDING CAPACITIES; BINDING CONSTANT; BINDING KINETICS; BIOTECH PRODUCTS; CHEMICAL MICROARRAYS; CULTURE SUPERNATANT; EQUILIBRIUM BINDING; HUMAN ANTIBODIES; LIGHT CHAIN; MURINE MONOCLONAL ANTIBODY; PHYSIOLOGICAL PH; PROTEIN A; RECOVERY YIELD; SMALL MOLECULES; STANDARD PROTEINS; STANDARD PROTOCOLS; SUPERNATANTS; THERAPEUTIC ANTIBODIES;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANIMAL CELL CULTURE; BINDERS; EQUILIBRIUM CONSTANTS; GEL PERMEATION CHROMATOGRAPHY; MOLECULES; MONOCLONAL ANTIBODIES; ORGANIC SOLVENTS; PH; PROTEINS; PURIFICATION;

LIGANDS;

2A10 ANTIBODY; BEVACIZUMAB; GLYCINE; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN KAPPA CHAIN; LIGAND; MDJ8 ANTIBODY; MONOCLONAL ANTIBODY; PALIVIZUMAB; PROTEIN A; RECOMBINANT ANTIBODY; SMALL MOLECULE AFFINITY LIGAND; TOCILIZUMAB; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTIBODY DETECTION; ARTICLE; BINDING AFFINITY; BINDING KINETICS; CONCENTRATION PROCESS; CONTROLLED STUDY; DRUG PURIFICATION; DRUG SELECTIVITY; DRUG STRUCTURE; ELUTION; INTERMETHOD COMPARISON; LIGAND BINDING; MICROARRAY ANALYSIS; MOUSE; NONHUMAN; PH; PRIORITY JOURNAL; SUPERNATANT;

ANIMALS; ANTIBODIES; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROGEN-ION CONCENTRATION; MICROARRAY ANALYSIS; OSMOLAR CONCENTRATION; PROTEIN BINDING; PROTEIN STABILITY; RABBITS; RECOMBINANT PROTEINS; SEPHAROSE; SMALL MOLECULE LIBRARIES;

MURINAE;

EID: 79959568074     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.05.040     Document Type: Article

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