A high-throughput assay for HIV-1 integrase 3'-processing activity using time-resolved fluorescence

Journal of Virological Methods

Volumn 184, Issue 1-2, 2012, Pages 34-40

  Han, Ying Shan ^a   Quashie, Peter ^a,b   Mesplede, Thibault ^a   Xu, Hongtao ^a   Mekhssian, Kevork ^c   Fenwick, Craig ^c   Wainberg, Mark A ^a,b  

^a MCGILL UNIVERSITY   (Canada)

^b MCGILL UNIVERSITY   (Canada)

^c BOEHRINGER INGELHEIM CANADA LTD   (Canada)

Author keywords

3' Processing; HIV 1 integrase; Integrase inhibitors; Time resolved fluorescence

Indexed keywords

INTEGRASE; INTEGRASE INHIBITOR; METAL ION; OLIGONUCLEOTIDE; VIRUS DNA;

ARTICLE; CATALYSIS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA PURIFICATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; HOST CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; REPRODUCIBILITY; TIME RESOLVED FLUORESCENCE; VIRUS CELL INTERACTION; VIRUS GENOME;

FLUORESCENCE; HIGH-THROUGHPUT SCREENING ASSAYS; HIV INTEGRASE; HIV INTEGRASE INHIBITORS; HIV-1; HUMANS; TIME FACTORS;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84863832436     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.05.003     Document Type: Article

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