Integrating macrophages into organotypic co-cultures: A 3D in vitro model to study tumor-associated macrophages

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Linde, Nina ^a   Gutschalk, Claudia M ^a   Hoffmann, Claudia ^b   Yilmaz, Dilan ^a   Mueller, Margareta M ^a,b  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b FURTWANGEN UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN TYPE 1; GELATINASE A; GELATINASE B; INTERLEUKIN 4; LIPOPOLYSACCHARIDE; RECOMBINANT GAMMA INTERFERON;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BASEMENT MEMBRANE; CANCER CELL CULTURE; CANCER GROWTH; CANCER INVASION; CANCER MODEL; CELL INTERACTION; CELL POLARITY; COCULTURE; COLLAGEN DEGRADATION; CONTROLLED STUDY; CULTURE MEDIUM; HUMAN; HUMAN CELL; IN VITRO STUDY; MACROPHAGE ACTIVATION; MACROPHAGE POLARIZATION; MOUSE; NONHUMAN; ORGANOTYPIC TUMOR COCULTURE; PROTEIN DEGRADATION; SKIN CARCINOMA; SKIN FIBROBLAST; THREE DIMENSIONAL IMAGING; TUMOR ASSOCIATED LEUKOCYTE; TUMOR MICROENVIRONMENT;

ANIMALS; CARCINOMA, SQUAMOUS CELL; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; COCULTURE TECHNIQUES; HUMANS; INTERLEUKIN-4; MACROPHAGES; MICE; NEOPLASM INVASIVENESS; TISSUE CULTURE TECHNIQUES; TUMOR CELLS, CULTURED;

MURINAE;

EID: 84863692895     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0040058     Document Type: Article

References (42)

1. Mueller MM, Fusenig NE, (2004) Friends or foes- bipolar effects of the tumour stroma in cancer. Nat Rev Cancer 4: 839-849.
2. Smola H, Stark HJ, Thiekotter G, Mirancea N, Krieg T, et al. (1998) Dynamics of basement membrane formation by keratinocyte-fibroblast interactions in organotypic skin culture. Exp Cell Res 239: 399-410.
3. Hirschhaeuser F, Menne H, Dittfeld C, West J, Mueller-Klieser W, et al. (2010) Multicellular tumor spheroids: an underestimated tool is catching up again. J Biotechnol 148: 3-15.
4. Vaira V, Fedele G, Pyne S, Fasoli E, Zadra G, et al. (2010) Preclinical model of organotypic culture for pharmacodynamic profiling of human tumors. Proc Natl Acad Sci U S A 107: 8352-8356.
5. Lin EY, Li JF, Bricard G, Wang W, Deng Y, et al. (2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages. Mol Oncol 1: 288-302.
6. Stark HJ, Szabowski A, Fusenig NE, Maas-Szabowski N, (2004) Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system. Biol Proced Online 6: 55-60.
7. Schoop VM, Mirancea N, Fusenig NE, (1999) Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts. J Invest Dermatol 112: 343-353.
8. Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, et al. (2000) c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cell 103: 745-755.
9. Stark HJ, Boehnke K, Mirancea N, Willhauck MJ, Pavesio A, et al. (2006) Epidermal homeostasis in long-term scaffold-enforced skin equivalents. J Investig Dermatol Symp Proc 11: 93-105.
10. Obermueller E, Vosseler S, Fusenig NE, Mueller MM, (2004) Cooperative autocrine and paracrine functions of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in the progression of skin carcinoma cells. Cancer Res 64: 7801-7812.
11. Gutschalk CM, Herold-Mende CC, Fusenig NE, Mueller MM, (2006) Granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor promote malignant growth of cells from head and neck squamous cell carcinomas in vivo. Cancer Res 66: 8026-8036.
12. Kalluri R, Zeisberg M, (2006) Fibroblasts in cancer. Nat Rev Cancer 6: 392-401.
13. Lederle W, Depner S, Schnur S, Obermueller E, Catone N, et al. (2011) IL-6 promotes malignant growth of skin SCCs by regulating a network of autocrine and paracrine cytokines. Int J Cancer 128: 2803-2814.
14. Mantovani A, Bottazzi B, Colotta F, Sozzani S, Ruco L, (1992) The origin and function of tumor-associated macrophages. Immunology Today 13: 265-270.
15. Pollard JW, (2009) Trophic macrophages in development and disease. Nature Reviews Immunology 9: 259-270.
16. Allavena P, Sica A, Garlanda C, Mantovani A, (2008) The Yin-Yang of tumor-associated macrophages in neoplastic progression and immune surveillance. Immunol Rev 222: 155-161.
17. Sica A, Schioppa T, Mantovani A, Allavena P, (2006) Tumour-associated macrophages are a distinct M2 polarised population promoting tumour progression: potential targets of anti-cancer therapy. Eur J Cancer 42: 717-727.
18. Fusenig NE, Breitkreutz D, Dzarlieva RT, Boukamp P, Bohnert A, et al. (1983) Growth and differentiation characteristics of transformed keratinocytes from mouse and human skin in vitro and in vivo. J Invest Dermatol 81: 168s-175s.
19. Fusenig NE, Boukamp P, (1998) Multiple stages and genetic alterations in immortalization, malignant transformation, and tumor progression of human skin keratinocytes. Mol Carcinog 23: 144-158.
20. Stacey A, Doyle A, (1997) Routine testing of cell cultures and their products for mycoplasma contamination. Methods Mol Biol 75: 305-311.
21. Lederle W, Linde N, Heusel J, Bzyl J, Woenne EC, et al. (2010) Platelet-derived growth factor-B normalizes micromorphology and vessel function in vascular endothelial growth factor-A-induced squamous cell carcinomas. Am J Pathol 176: 981-994.
22. Mantovani A, Sozzani S, Locati M, Allavena P, Sica A, (2002) Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends Immunol 23: 549-555.
23. Solinas G, Schiarea S, Liguori M, Fabbri M, Pesce S, et al. (2010) Tumor-conditioned macrophages secrete migration-stimulating factor: a new marker for M2-polarization, influencing tumor cell motility. J Immunol 185: 642-652.
24. Schledzewski K, Falkowski M, Moldenhauer G, Metharom P, Kzhyshkowska J, et al. (2006) Lymphatic endothelium-specific hyaluronan receptor LYVE-1 is expressed by stabilin-1+, F4/80+, CD11b+ macrophages in malignant tumours and wound healing tissue in vivo and in bone marrow cultures in vitro: implications for the assessment of lymphangiogenesis. J Pathol 209: 67-77.
25. Kuebler JP, Oberley TD, Meisner LF, Sidky YA, Reznikoff CA, et al. (1987) Effect of interferon alpha, interferon beta, and interferon gamma on the in vitro growth of human renal adenocarcinoma cells. Invest New Drugs 5: 21-29.
26. Yip I, Pang XP, Berg L, Hershman JM, (1995) Antitumor actions of interferon-gamma and interleukin-1 beta on human papillary thyroid carcinoma cell lines. J Clin Endocrinol Metab 80: 1664-1669.
27. Pettersen JS, Fuentes-Duculan J, Suarez-Farinas M, Pierson KC, Pitts-Kiefer A, et al. (2011) Tumor-associated macrophages in the cutaneous SCC microenvironment are heterogeneously activated. J Invest Dermatol 131: 1322-1330.
28. Egeblad M, Rasch MG, Weaver VM, (2010) Dynamic interplay between the collagen scaffold and tumor evolution. Curr Opin Cell Biol 22: 697-706.
29. Bechetoille N, Vachon H, Gaydon A, Boher A, Fontaine T, et al. (2011) A new organotypic model containing dermal-type macrophages. Exp Dermatol 20: 1035-1037.
30. Ogawa K, Funaba M, Chen Y, Tsujimoto M, (2006) Activin A functions as a Th2 cytokine in the promotion of the alternative activation of macrophages. J Immunol 177: 6787-6794.
31. Pesce J, Kaviratne M, Ramalingam TR, Thompson RW, Urban JF Jr, et al. (2006) The IL-21 receptor augments Th2 effector function and alternative macrophage activation. J Clin Invest 116: 2044-2055.
32. Roca H, Varsos ZS, Sud S, Craig MJ, Ying C, et al. (2009) CCL2 and interleukin-6 promote survival of human CD11b+ peripheral blood mononuclear cells and induce M2-type macrophage polarization. J Biol Chem 284: 34342-34354.
33. Pollard JW, (2008) Macrophages define the invasive microenvironment in breast cancer. J Leukoc Biol 84: 623-630.
34. Hornung J, Bohnert A, Phan-Than L, Krieg T, Fusenig NE, (1987) Basement membrane formation by malignant mouse keratinocyte cell lines in organotypic culture and transplants: correlation with degree of morphologic differentiation. J Cancer Res Clin Oncol 113: 325-341.
35. Levental KR, Yu H, Kass L, Lakins JN, Egeblad M, et al. (2009) Matrix crosslinking forces tumor progression by enhancing integrin signaling. Cell 139: 891-906.
36. Butcher DT, Alliston T, Weaver VM, (2009) A tense situation: forcing tumour progression. Nat Rev Cancer 9: 108-122.
37. Barron L, Wynn TA, (2011) Fibrosis is regulated by Th2 and Th17 responses and by dynamic interactions between fibroblasts and macrophages. Am J Physiol Gastrointest Liver Physiol 300: G723-728.
38. Friedl P, Gilmour D, (2009) Collective cell migration in morphogenesis, regeneration and cancer. Nat Rev Mol Cell Biol 10: 445-457.
39. Mueller MM, Fusenig NE, (1999) Constitutive expression of G-CSF and GM-CSF in human skin carcinoma cells with functional consequence for tumor progression. Int J Cancer 83: 780-789.
40. Livera G, Delbes G, Pairault C, Rouiller-Fabre V, Habert R, (2006) Organotypic culture, a powerful model for studying rat and mouse fetal testis development. Cell Tissue Res 324: 507-521.
41. Fernandez-Bueno I, Pastor JC, Gayoso MJ, Alcalde I, Garcia MT, (2008) Muller and macrophage-like cell interactions in an organotypic culture of porcine neuroretina. Mol Vis 14: 2148-2156.
42. Brana C, Biggs TE, Mann DA, Sundstrom LE, (1999) A macrophage hippocampal slice co-culture system: application to the study of HIV-induced brain damage. J Neurosci Methods 90: 7-11.