A highly basic sequence at the N-terminal region is essential for targeting the DNA replication protein ORC1 to the nucleus in Leishmania donovani

Microbiology (United Kingdom)

Volumn 158, Issue 7, 2012, Pages 1775-1782

  Kumar, Devanand ^a   Kumar, Diwakar ^a,b   Saha, Swati ^a  

^a UNIVERSITY OF DELHI   (India)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; DNA TOPOISOMERASE (ATP HYDROLYSING); ORC1 PROTEIN; PHOSPHODIESTERASE I; PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; LEISHMANIA DONOVANI; LEISHMANIA MAJOR; MUTATION; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; VISCERAL LEISHMANIASIS;

CELL NUCLEUS; DNA, PROTOZOAN; LEISHMANIA DONOVANI; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; NUCLEAR LOCALIZATION SIGNALS; ORIGIN RECOGNITION COMPLEX; PROTEIN TRANSPORT; SEQUENCE ANALYSIS, DNA;

EUKARYOTA; LEISHMANIA DONOVANI; LEISHMANIA MAJOR; TETRA;

EID: 84863643195     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.055640-0     Document Type: Article

References (19)

1. Banerjee, B., Roy, A., Sen, N. & Majumder, H. K. (2010). A tyrosyl DNA phosphodiesterase 1 from kinetoplastid parasite Leishmania donovani (LdTdp1) capable of removing topo I-DNA covalent complexes. Mol Microbiol 78, 119-137.
2. Dubessay, P., Blaineau, C., Bastien, P., Tasse, L., Van Dijk, J., Crobu, L. & Pagès, M. (2006). Cell cycle-dependent expression regulation by the proteasome pathway and characterization of the nuclear targeting signal of a Leishmania major Kin-13 kinesin. Mol Microbiol 59, 1162-1174.
3. Godoy, P. D., Nogueira-Junior, L. A., Paes, L. S., Cornejo, A., Martins, R. M., Silber, A. M., Schenkman, S. & Elias, M. C. (2009). Trypanosome prereplication machinery contains a single functional orc1/cdc6 protein, which is typical of archaea. Eukaryot Cell 8, 1592-1603.
4. Görlich, D. (1997). Nuclear protein import. Curr Opin Cell Biol 9, 412-419.
5. Görlich, D., Panté, N., Kutay, U., Aebi, U. & Bischoff, F. R. (1996). Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J 15, 5584-5594.
6. Ha, D. S., Schwarz, J. K., Turco, S. J. & Beverley, S. M. (1996). Use of the green fluorescent protein as a marker in transfected Leishmania. Mol Biochem Parasitol 77, 57-64.
7. Hoek, M., Engstler, M. & Cross, G. A. (2000). Expression-siteassociated gene 8 (ESAG8) of Trypanosoma brucei is apparently essential and accumulates in the nucleolus. J Cell Sci 113, 3959-3968.
8. Ivens, A. C., Peacock, C. S., Worthey, E. A., Murphy, L., Aggarwal, G., Berriman, M., Sisk, E., Rajandream, M. A., Adlem, E. & other authors (2005). The genome of the kinetoplastid parasite, Leishmania major. Science 309, 436-442.
9. Kumar, D., Mukherji, A. & Saha, S. (2008). Expression and subcellular localization of ORC1 in Leishmania major. Biochem Biophys Res Commun 375, 74-79.
10. Kumar, D., Minocha, N., Rajanala, K. & Saha, S. (2009). The distribution pattern of proliferating cell nuclear antigen in the nuclei of Leishmania donovani. Microbiology 155, 3748-3757.
11. Lufei, C. & Cao, X. (2009). Nuclear import of Pin1 is mediated by a novel sequence in the PPIase domain. FEBS Lett 583, 271-276.
12. Marchetti, M. A., Tschudi, C., Kwon, H., Wolin, S. L. & Ullu, E. (2000). Import of proteins into the trypanosome nucleus and their distribution at karyokinesis. J Cell Sci 113, 899-906.
13. Nigg, E. A. (1997). Nucleocytoplasmic transport: signals, mechanisms and regulation. Nature 386, 779-787.
14. Peacock, C. S., Seeger, K., Harris, D., Murphy, L., Ruiz, J. C., Quail, M. A., Peters, N., Adlem, E., Tivey, A. & other authors (2007). Comparative genomic analysis of three Leishmania species that cause diverse human disease. Nat Genet 39, 839-847.
15. Powers, M. A. & Forbes, D. J. (1994). Cytosolic factors in nuclear transport: what's importin? Cell 79, 931-934.
16. Robinson, K. A. & Beverley, S. M. (2003). Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite Leishmania. Mol Biochem Parasitol 128, 217-228.
17. Sengupta, T., Mukherjee, M., Mandal, C., Das, A. & Majumder, H. K. (2003). Functional dissection of the C-terminal domain of type II DNA topoisomerase from the kinetoplastid hemoflagellate Leishmania donovani. Nucleic Acids Res 31, 5305-5316.
18. Waterhouse, A. M., Procter, J. B., Martin, D. M., Clamp, M. & Barton, G. J. (2009). Jalview Version 2-a multiple sequence alignment editor and analysis workbench. Bioinformatics 25, 1189-1191.
19. Westergaard, G. G., Bercovich, N., Reinert, M. D. & Vazquez, M. P. (2010). Analysis of a nuclear localization signal in the p14 splicing factor in Trypanosoma cruzi. Int J Parasitol 40, 1029-1035.