Analysis of a nuclear localization signal in the p14 splicing factor in Trypanosoma cruzi

International Journal for Parasitology

Volumn 40, Issue 9, 2010, Pages 1029-1035

  Westergaard, Gaston G ^b   Bercovich, Natalia ^a,c   Reinert, Marina D ^b   Vazquez, Martin P ^a,b  

^a INSTITUTO DE INVESTIGACIONES EN INGENIERÍA GENÉTICA Y BIOLOGÍA MOLECULAR   (Argentina)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^c Max Planck Institute for Intelligent Systems   (Germany)

Author keywords

Gateway vector; NLS; P14; PTREX; Splicing factor; Trypanosoma

Indexed keywords

CELL PROTEIN; GREEN FLUORESCENT PROTEIN; PROTEIN P14; PROTEIN SF3B155; UNCLASSIFIED DRUG; NUCLEAR LOCALIZATION SIGNAL; NUCLEAR PROTEIN; PROTOZOAL PROTEIN; RECOMBINANT PROTEIN; RNA BINDING PROTEIN;

AMINO ACID; MAPPING; PROTEIN; RNA;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL NUCLEUS; COMPLEX FORMATION; CONTROLLED STUDY; GENETIC TRANSFECTION; MOLECULAR RECOGNITION; MOLECULAR SIZE; NONHUMAN; NUCLEAR IMPORT; NUCLEAR LOCALIZATION SIGNAL; PEPTIDE MAPPING; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; PROTEIN MOTIF; RNA SPLICING; TRYPANOSOMA CRUZI; YEAST; ENZYMOLOGY; GENETICS; METABOLISM; PROTEIN TRANSPORT;

AMINO ACID MOTIFS; GREEN FLUORESCENT PROTEINS; NUCLEAR LOCALIZATION SIGNALS; NUCLEAR PROTEINS; PROTEIN TRANSPORT; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; RNA-BINDING PROTEINS; TRYPANOSOMA CRUZI;

TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

EID: 77953916108     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2010.02.011     Document Type: Article

References (24)

1. Avila M.L., Bercovich N., Westergaard G., Levin M.J., Vázquez M.P. Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi. Biochem. Biophys. Res. Commun. 2007, 364:26-32.
2. Ben-Dov C.P., Levin M.J., Vázquez M.P. Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi. Mol. Biochem. Parasitol. 2005, 140:97-105.
3. Bercovich N., Levin M., Clayton C., Vazquez M.P. Identification of core components of the exon junction complex in trypanosomes. Mol. Biochem. Parasitol. 2009, 166:190-193.
4. Bercovich N., Levin M.J., Vazquez M.P. The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30. Biochem. Biophys. Res. Commun. 2009, 380:850-855.
5. Cassola, A., Frasch, A., 2009. An RNA recognition motif mediates the nucleocytoplasmic transport of a trypanosome RNA-binding protein. J. Biol. Chem. 1-20.
6. Clayton C.E. Life without transcriptional control? From fly to man and back again. EMBO J. 2002, 21:1881-1888.
7. Figueiredo L., Janzen C., Cross G., Gull K. A histone methyltransferase modulates antigenic variation in African Trypanosomes. Plos Biol. 2008, 6:e161.
8. Golas, M.M., Sander, B., Will, C.L., Lührmann, R., Stark, H., 2003. Molecular architecture of the multiprotein splicing factor SF3b. Science 300, 980-984.
9. Görlich D. Nuclear protein import. Curr. Opin. Cell Biol. 1997, 9:412-419.
10. Hoek M., Engstler M., Cross G.A. Expression-site-associated gene 8 (ESAG8) of Trypanosoma brucei is apparently essential and accumulates in the nucleolus. J. Cell Sci. 2000, 113(Pt 22):3959-3968.
11. Kuwasako K., Dohmae N., Inoue M., Shirouzu M., Taguchi S., Güntert P., Séraphin B., Muto Y., Yokoyama S. Complex assembly mechanism and an RNA-binding mode of the human p14-SF3b155 spliceosomal protein complex identified by NMR solution structure and functional analyses. Proteins 2001, 71:1617-1636.
12. Liang X.H., Haritan A., Uliel S., Michaeli S. Trans and cis splicing in trypanosomatids: mechanism factors and regulation. Euk. Cell 2003, 2:830-840.
13. Lufei C., Cao X. Nuclear import of Pin1 is mediated by a novel sequence in the PPIase domain. FEBS Lett. 2009, 583:271-276.
14. Marchetti M.A., Tschudi C., Kwon H., Wolin S.L., Ullu E. Import of proteins into the trypanosome nucleus and their distribution at karyokinesis. J. Cell Sci. 2000, 113(Pt 5):899-906.
15. Muñoz-Fontela C., Rodríguez E., Nombela C., Arroyo J., Rivas C. Characterization of the bipartite nuclear localization signal of protein LANA2 from Kaposi's sarcoma-associated herpesvirus. Biochem. J. 2003, 374:545-550.
16. Nwaka S., Hudson A. Innovative lead discovery strategies for tropical diseases. Nat. Rev. Drug Discov. 2006, 5:941-955.
17. Rosenblum J., Pemberton L., Bonifaci N., Blobel G. Nuclear import and the evolution of a multifunctional RNA-binding protein. J. Cell. Biol. 1998, 143:887.
18. Schellenberg M.J., Edwards R.A., Ritchie D.B., Kent O.A., Golas M.M., Stark H., Lührmann R., Glover J.N., MacMillan A.M. Crystal structure of a core spliceosomal protein interface. Proc. Natl. Acad. Sci. USA 2006, 103:1266-1271.
19. Spadaccini R., Reidt U., Dybkov O., Will C., Frank R., Stier G., Corsini L., Wahl M.C., Lührmann R., Sattler M. Biochemical and NMR analyses of an SF3b155-p14-U2AF-RNA interaction network involved in branch point definition during pre-mRNA splicing. RNA 2006, 12:410-425.
20. Thickman K.R., Swenson M.C., Kabogo J.M., Gryczynski Z., Kielkopf C.L. Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors. J. Mol. Biol. 2006, 356:664-683.
21. Vazquez M., Atorrasagasti C., Bercovich N., Volcovich R., Levin M.J. Unique features of the Trypanosoma cruzi U2AF35 splicing factor. Mol. Biochem. Parasitol. 2003, 128:77-81.
22. Vazquez M., Mualem D., Bercovich N., Stern M., Nyambega B., Barda O., Masiga D., Gupta S., Michaeli S., Levin M. Functional characterization and protein-protein interactions of trypanosome splicing factors U2AF35, U2AF65 and SF1. Mol. Biochem. Parasitol. 2009, 164:137-146.
23. Vazquez M.P., Levin M.J. Functional analysis of the intergenic regions of TcP2β gene loci allowed the construction of an improved Trypanosoma cruzi expression vector. Gene 1999, 239:217-225.
24. Villanova G., Nardelli S., Cribb P., Magdaleno A., Silber A., Motta M.C.M., Schenkman S., Serra E. Trypanosoma cruzi bromodomain factor 2 (BDF2) binds to acetylated histones and is accumulated after UV irradiation. Int. J. Parasitol. 2009, 39:665-673.