Development of an optimized tetracycline-inducible expression system to increase the accumulation of interleukin-10 in tobacco BY-2 suspension cells

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Bortesi, Luisa ^a,c   Rademacher, Thomas ^b   Schiermeyer, Andreas ^b   Schuster, Flora ^c   Pezzotti, Mario ^a   Schillberg, Stefan ^b  

^a UNIVERSITY OF VERONA   (Italy)

^b FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BASIC RESEARCH; BINARY VECTORS; BY-2 CELL; CULTURE MEDIUM; EXPRESSION SYSTEM; GROWTH PHASE; INDUCIBLE PROMOTER; INDUSTRIAL PROTEINS; INTERLEUKIN-10; MARKER GENES; MOLECULAR FARMING; PLANT CELL SUSPENSION CULTURES; PLANT CELLS; PLANT GROWTH; PLATFORM TECHNOLOGY; PROTEOLYTIC ACTIVITIES; STABLE TRANSFORMATION; SUSPENSION CELL; TEMPORAL RESTRICTIONS; TOBACCO BY-2; TRANSGENE EXPRESSION;

CELLS; CYTOLOGY; GENES; OPTIMIZATION; PLANT CELL CULTURE; PLANT LIFE EXTENSION; RECOMBINANT PROTEINS; TOBACCO;

SUSPENSIONS (COMPONENTS);

INTERLEUKIN 10; TETRACYCLINE; RECOMBINANT PROTEIN; TRANSACTIVATOR PROTEIN;

5' UNTRANSLATED REGION; ARTICLE; BRIGHT YELLOW 2 CELL; CELL GROWTH; CELL SUSPENSION; CELL TYPE; CELL VIABILITY; GENETIC MARKER; PROTEIN DEGRADATION; PROTEIN EXPRESSION; TOBACCO; BIOSYNTHESIS; CAULIFLOWER MOSAIC VIRUS; COCULTURE; CYTOLOGY; DRUG EFFECT; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PLANT CELL; PROMOTER REGION; RHIZOBIUM RADIOBACTER; TRANSGENIC PLANT;

NICOTIANA TABACUM;

5' UNTRANSLATED REGIONS; AGROBACTERIUM TUMEFACIENS; CAULIMOVIRUS; COCULTURE TECHNIQUES; GENETIC VECTORS; INTERLEUKIN-10; PLANT CELLS; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; TETRACYCLINE; TOBACCO; TRANS-ACTIVATORS;

EID: 84863600664     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-40     Document Type: Article

References (42)

1. Schiermeyer A, Schillberg S. Pharmaceuticals. Genetic modification of plants: agriculture, horticulture and forestry 2010, 221-235. Springer, Berlin Heidelberg, Kempken F, Jung C.
2. Faye L, Gomord V. Success stories in molecular farming-a brief overview. Plant Biotechnol J 2010, 8(5):525-528. 10.1111/j.1467-7652.2010.00521.x, 20500680.
3. Bock R, Warzecha H. Solar-powered factories for new vaccines and antibiotics. Trends Biotechnol 2010, 28(5):246-252. 10.1016/j.tibtech.2010.01.006, 20207435.
4. Peters J, Stoger E. Transgenic crops for the production of recombinant vaccines and anti-microbial antibodies. Hum Vaccin 2011, 7:3.
5. Tremblay R, Wang D, Jevnikar AM, Ma S. Tobacco, a highly efficient green bioreactor for production of therapeutic proteins. Biotechnol Adv 2010, 28(2):214-221. 10.1016/j.biotechadv.2009.11.008, 19961918.
6. Obembe OO, Popoola JO, Leelavathi S, Reddy SV. Advances in plant molecular farming. Biotechnol Adv 2011, 29(2):210-222. 10.1016/j.biotechadv.2010.11.004, 21115109.
7. Zhong J. Plant cells (Advances in biochemical engineering-biotechnology) 2001, Springer, Berlin Heidelberg.
8. Schiermeyer A, Schinkel H, Apel S, Fischer R, Schillberg S. Production of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAalpha1) in tobacco is hampered by proteolysis. Biotechnol Bioeng 2005, 89(7):848-858. 10.1002/bit.20410, 15685597.
9. Huang TK, Plesha MA, McDonald KA. Semicontinuous bioreactor production of a recombinant human therapeutic protein using a chemically inducible viral amplicon expression system in transgenic plant cell suspension cultures. Biotechnol Bioeng 2010, 106(3):408-421.
10. Sorrentino A, Schillberg S, Fischer R, Porta R, Mariniello L. Molecular farming of human tissue transglutaminase in tobacco plants. Amino Acids 2009, 36(4):765-772. 10.1007/s00726-008-0132-8, 18594943.
11. Avesani L, Vitale A, Pedrazzini E, Devirgilio M, Pompa A, Barbante A, Gecchele E, Dominici P, Morandini F, Brozzetti A, Falorni A, Pezzotti M. Recombinant human GAD65 accumulates to high levels in transgenic tobacco plants when expressed as an enzymatically inactive mutant. Plant Biotechnol J 2010, 8(8):862-872. 10.1111/j.1467-7652.2010.00514.x, 20374524.
12. Zhou F, Badillo-Corona JA, Karcher D, Gonzalez-Rabade N, Piepenburg K, Borchers AM, Maloney AP, Kavanagh TA, Gray JC, Bock R. High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes. Plant Biotechnol J 2008, 6(9):897-913. 10.1111/j.1467-7652.2008.00356.x, 19548344.
13. Ehsani P, Meunier A, Nato F, Jafari A, Nato A, Lafaye P. Expression of anti human IL-4 and IL-6 scFvs in transgenic tobacco plants. Plant Mol Biol 2003, 52(1):17-29. 10.1023/A:1023902407855, 12825686.
14. Bortesi L, Rossato M, Schuster F, Raven N, Stadlmann J, Avesani L, Falorni A, Bazzoni F, Bock R, Schillberg S, Pezzotti M. Viral and murine interleukin-10 are correctly processed and retain their biological activity when produced in tobacco. BMC Biotechnol 2009, 9:22. 10.1186/1472-6750-9-22, 2667500, 19298643.
15. Corrado G, Karali M. Inducible gene expression systems and plant biotechnology. Biotechnol Adv 2009, 27(6):733-743. 10.1016/j.biotechadv.2009.05.006, 19460424.
16. Padidam M. Chemically regulated gene expression in plants. Curr Opin Plant Biol 2003, 6(2):169-177. 10.1016/S1369-5266(03)00005-0, 12667875.
17. David KM, Perrot-Rechenmann C. Characterization of a tobacco Bright Yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression. Plant Physiol 2001, 125(4):1548-1553. 10.1104/pp.125.4.1548, 1539379, 11299335.
18. Gatz C, Lenk I. Promoters that respond to chemical inducers. Trends Plant Sci 1998, 3(9):352-358.
19. David KM, Couch D, Braun N, Brown S, Grosclaude J, Perrot-Rechenmann C. The auxin-binding protein 1 is essential for the control of cell cycle. Plant J 2007, 50(2):197-206. 10.1111/j.1365-313X.2007.03038.x, 17376160.
20. Jach G, Binot E, Frings S, Luxa K, Schell J. Use of red fluorescent protein from Discosoma sp. (dsRED) as a reporter for plant gene expression. Plant J 2001, 28(4):483-491.
21. Rademacher T, Hausler RE, Hirsch HJ, Zhang L, Lipka V, Weier D, Kreuzaler F, Peterhansel C. An engineered phosphoenolpyruvate carboxylase redirects carbon and nitrogen flow in transgenic potato plants. Plant J 2002, 32(1):25-39. 10.1046/j.1365-313X.2002.01397.x, 12366798.
22. Allen GC, Hall G, Michalowski S, Newman W, Spiker S, Weissinger AK, Thompson WF. High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco. Plant Cell 1996, 8(5):899-913. 161147, 8672887.
23. Carrington JC, Freed DD. Cap-inependent enhancement of translation by a plant potyvirus 5′ nontranslated region. J Virol 1990, 64(4):1590-1597. 249294, 2319646.
24. Bowman SM, Drzewiecki KE, Mojica ER, Zielinski AM, Siegel A, Aga DS, Berry JO. Toxicity and reductions in intracellular calcium levels following uptake of a tetracycline antibiotic in Arabidopsis. Environ Sci Technol 2011, 45(20):8958-8964. 10.1021/es200863j, 21882870.
25. Plasson C, Michel R, Lienard D, Saint-Jore-Dupas C, Sourrouille C, de March G, Gomord V. Production of recombinant proteins in suspension-cultured plant cells. Methods Mol Biol 2009, 483:145-161. 10.1007/978-1-59745-407-0_9, 19183898.
26. Xu J, Ge X, Dolan MC. Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures. Biotechnol Adv 2011, 29(3):278-299. 10.1016/j.biotechadv.2011.01.002, 21236330.
27. Weathers PJ, Towler MJ, Xu J. Bench to batch: advances in plant cell culture for producing useful products. Appl Microbiol Biotechnol 2010, 85(5):1339-1351. 10.1007/s00253-009-2354-4, 19956945.
28. Hellwig S, Drossard J, Twyman RM, Fischer R. Plant cell cultures for the production of recombinant proteins. Nat Biotechnol 2004, 22(11):1415-1422. 10.1038/nbt1027, 15529167.
29. Sack M, Paetz A, Kunert R, Bomble M, Hesse F, Stiegler G, Fischer R, Katinger H, Stoeger E, Rademacher T. Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2 F5 produced in transgenic BY-2 suspension cultures. FASEB J 2007, 21(8):1655-1664. 10.1096/fj.06-5863com, 17327362.
30. Gatz C, Quail PH. Tn10-encoded tet repressor can regulate an operator-containing plant promoter. Proc Natl Acad Sci USA 1988, 85(5):1394-1397. 10.1073/pnas.85.5.1394, 279777, 2830617.
31. Iglesias VA, Moscone EA, Papp I, Neuhuber F, Michalowski S, Phelan T, Spiker S, Matzke M, Matzke AJ. Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco. Plant Cell 1997, 9(8):1251-1264. 156995, 9286104.
32. Halweg C, Thompson WF, Spiker S. The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: a flow cytometric study. Plant Cell 2005, 17(2):418-429. 10.1105/tpc.104.028100, 548816, 15659622.
33. Stoger E, Vaquero C, Torres E, Sack M, Nicholson L, Drossard J, Williams S, Keen D, Perrin Y, Christou P, Fischer R. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol Biol 2000, 42(4):583-590. 10.1023/A:1006301519427, 10809004.
34. Torres E, Vaquero C, Nicholson L, Sack M, Stoger E, Drossard J, Christou P, Fischer R, Perrin Y. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies. Transgenic Res 1999, 8(6):441-449. 10.1023/A:1008969031219, 10767987.
35. Vaquero C, Sack M, Schuster F, Finnern R, Drossard J, Schumann D, Reimann A, Fischer R. A carcinoembryonic antigen-specific diabody produced in tobacco. FASEB J 2002, 16(3):408-410.
36. Kay R, Chan A, Daly M, McPherson J. Duplication of CaMV 35 S Promoter Sequences Creates a Strong Enhancer for Plant Genes. Science 1987, 236(4806):1299-1302. 10.1126/science.236.4806.1299, 17770331.
37. Huang J, Wu L, Yalda D, Adkins Y, Kelleher SL, Crane M, Lonnerdal B, Rodriguez RL, Huang N. Expression of functional recombinant human lysozyme in transgenic rice cell culture. Transgenic Res 2002, 11(3):229-239. 10.1023/A:1015663706259, 12113455.
38. Holland T, Sack M, Rademacher T, Schmale K, Altmann F, Stadlmann J, Fischer R, Hellwig S. Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture. Biotechnol Bioeng 2010, 107(2):278-289. 10.1002/bit.22800, 20506104.
39. Rademacher T, Sack M, Arcalis E, Stadlmann J, Balzer S, Altmann F, Quendler H, Stiegler G, Kunert R, Fischer R, Stoger E. Recombinant antibody 2 G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans. Plant Biotechnol J 2008, 6(2):189-201. 10.1111/j.1467-7652.2007.00306.x, 17979949.
40. Janus D, Hoff B, Hofmann E, Kuck U. An efficient fungal RNA-silencing system using the DsRed reporter gene. Appl Environ Microbiol 2007, 73(3):962-970. 10.1128/AEM.02127-06, 1800780, 17142377.
41. Wittamer V, Bertrand JY, Gutschow PW, Traver D. Characterization of the mononuclear phagocyte system in zebrafish. Blood 2011, 117(26):7126-7135. 10.1182/blood-2010-11-321448, 21406720.
42. Schiermeyer A, Hartenstein H, Mandal MK, Otte B, Wahner V, Schillberg S. A membrane-bound matrix-metalloproteinase from Nicotiana-tabacum cv. BY-2 is induced by bacterial pathogens. BMC Plant Biol 2009, 9:83. 10.1186/1471-2229-9-83, 2715019, 19563670.